CHROMATOGRAPHIC CHARACTERISTICS OF SOME AROMATIC COMPOUNDS OF BIOLOGICAL INTEREST

1961 ◽  
Vol 39 (3) ◽  
pp. 605-627 ◽  
Author(s):  
E. G. McGeer ◽  
M. C. Robertson ◽  
P. L. McGeer
Keyword(s):  
Phenolic Acids ◽  
Aromatic Compounds ◽  
Chromatographic Data ◽  
Biological Interest ◽  
Solvent Systems ◽  
Color Reactions ◽  
Spray Reagents

Paper chromatographic data are reported on 220 aromatic compounds of biological interest, including phenolic acids, indoles, imidazoles, purines, pyrimidines, and some miscellaneous substances. Rf data in four solvent systems and color reactions with 12 spray reagents are tabulated. Some correlations between structure of these compounds, relative Rf, and color with various spray reagents are noted.

A GENERAL CHROMATOGRAPHIC SURVEY OF AROMATIC COMPOUNDS OBTAINED FROM URINE

1959 ◽  
Vol 37 (12) ◽  
pp. 1493-1515 ◽  
Author(s):  
D. M. Smith ◽  
R. M. Paul ◽  
E. G. McGeer ◽  
P. L. McGeer
Keyword(s):  
Paper Chromatography ◽  
Aromatic Compounds ◽  
Two Dimensional ◽  
Spray Reagents

Aromatic compounds contained in both normal and schizophrenic urines have been extracted by several methods and the resultant extracts examined by two-dimensional paper chromatography with a variety of spray reagents. A comprehensive map plus tables summarize the data obtained on over 200 "aromatic" spots found in various urine extracts. Data are given on fractionation of the extracts. To allow comparison of one extract with another, an attempt was made to maintain quantitative control of the extraction, fractionation, and chromatographic procedures.

Analysis of Phenolic Acids by High Performance Liquid Chromatography Using a Step-Wise Gradient

1981 ◽  
Vol 36 (5-6) ◽  
pp. 357-360 ◽  
Author(s):  
Peter Proksch ◽  
Charles Wisdom ◽  
Eloy Rodriguez
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Intramolecular Hydrogen Bonding ◽  
High Performance Liquid Chromatographic ◽  
Cinnamic Acid Derivatives ◽  
Liquid Chromatographic Method ◽  
Percent Composition ◽  
Solvent Systems ◽  
Intramolecular Hydrogen ◽  
Liquid Chromatographic

Abstract A high performance liquid chromatographic method for analyzing phenolic acids on reverse phase C-18 is described. Twelve substituted benzoic and cinnamic acid derivatives as well as the parent acids were resolved in less than 25 min. Separation was achieved by using a step-wise gradient of three different solvent systems and by adding ammonium acetate for preventing intramolecular hydrogen bonding of the acids. The applicability of the described m ethod to crude plant extracts is demonstrated with Adenostoma fasciculatum, Adenostoma sparsifolium and Phyllospadix scouleri. The percent composition of the phenolic acids within the three extracts is given.

A GENERAL CHROMATOGRAPHIC SURVEY OF AROMATIC COMPOUNDS OBTAINED FROM URINE

1959 ◽  
Vol 37 (1) ◽  
pp. 1493-1515 ◽  
Author(s):  
D. M. Smith ◽  
R. M. Paul ◽  
E. G. McGeer ◽  
P. L. McGeer
Keyword(s):  
Paper Chromatography ◽  
Aromatic Compounds ◽  
Two Dimensional ◽  
Spray Reagents

Aromatic compounds contained in both normal and schizophrenic urines have been extracted by several methods and the resultant extracts examined by two-dimensional paper chromatography with a variety of spray reagents. A comprehensive map plus tables summarize the data obtained on over 200 "aromatic" spots found in various urine extracts. Data are given on fractionation of the extracts. To allow comparison of one extract with another, an attempt was made to maintain quantitative control of the extraction, fractionation, and chromatographic procedures.

The phenolic constituents from leaves of Manihot esculenta

1974 ◽  
Vol 52 (11) ◽  
pp. 2381-2386 ◽  
Author(s):  
M. L. Thakur ◽  
B. H. Somaroo ◽  
W. F. Grant
Keyword(s):  
Manihot Esculenta ◽  
Uv Light ◽  
Manihot Esculenta Crantz ◽  
Benzoic Acid Derivatives ◽  
Phenolic Constituents ◽  
Ferulic Acids ◽  
Color Reactions ◽  
Hydrolysis Of ◽  
Spray Reagents ◽  
Acid Esters

A total of 55 phenolic constituents was extracted with methanol – 1 % HCl at room temperature from dried leaves of cassava (Manihot esculenta Crantz). By means of thin-layer chromatographic and spectrophotometric techniques the 20 main phenolic constituents were identified as quercetin and luteolin glycosides, chlorogenic acid, esters of p-coumaric, caffeic, ferulic, and sinapic acids, and the glycosides of caffeic and ferulic acids. Benzoic acid derivatives were also confirmed. Acid and alkaline hydrolysis of the major compounds was carried out to determine their aglycones. Ultraviolet (UV) spectral data, Rf values, fluorescence in UV light, and color reactions with chromogenic spray reagents of the phenolic compounds and the aglycones are presented. The sugar residues of the major flavonoid compounds were identified as glucose.

scholarly journals Decomposition of Flavonols in the Presence of Saliva

2020 ◽  
Vol 10 (21) ◽  
pp. 7511
Author(s):  
Malgorzata Rogozinska ◽  
Magdalena Biesaga
Keyword(s):  
Hydrogen Peroxide ◽  
Phenolic Acids ◽  
Aromatic Compounds ◽  
Protocatechuic Acid ◽  
Benzoic Acid Derivatives ◽  
Dead End ◽  
End Products ◽  
Quercetin Glycosides ◽  
Human Digestive Tract ◽  
The Stability

In this study, the LC-MS/MS was applied to explore the stability of four common dietary flavonols, kaempferol, quercetin, isorhamnetin, and myricetin, in the presence of hydrogen peroxide and saliva. In addition, the influence of saliva on the representative quercetin glycosides, rutin, quercitrin, hyperoside, and spiraeoside was examined. Our study showed that, regardless of the oxidative agent used, flavonols stability decreases with increasing B-ring substitution. The decomposition of analyzed compounds was based on their splitting by the opening the heterocyclic C-ring and realizing more simple aromatic compounds. The dead-end products corresponded to different benzoic acid derivatives derived from B-ring. Kaempferol, quercetin, isorhamnetin, and myricetin were transformed into 4-hydroxybeznoic acid, protocatechuic acid, vanillic acid, and gallic acid, respectively. Additionally, for quercetin and myricetin, two intermediate depsides and 2,4,6-trihydroxybenzoic acid derived from A-ring were detected. All analyzed glycosides were resistant to hydrolysis in the presence of saliva. Based on our data, saliva was proven to be a next oxidative agent which leads to the formation of corresponding phenolic acids. Hence, studies on flavonols’ metabolism should take into consideration that the flavonols decomposition starts in the oral cavity; hence, in subsequent parts of the human digestive tract, they could be present not in their parent form but as phenolic acids. Further analyses of the influence of saliva on flavonols glycosides need to be performed due to the possible interindividual fluctuations.

Two-Dimensional Thin Layer Chromatographic Procedure for the Identification of Dye Intermediates in Arylamine Oxidation Hair Dyes

1966 ◽  
Vol 49 (5) ◽  
pp. 954-959 ◽  
Author(s):  
Christine M Kottemann
Keyword(s):  
Thin Layer ◽  
Acidic Solution ◽  
Chromatographic Study ◽  
Basic Solution ◽  
Two Dimensional ◽  
Hair Dyes ◽  
Initial Development ◽  
Chromatographic Procedure ◽  
Color Reactions ◽  
Spray Reagents

Abstract Dye intermediates in arylamine oxidation hair dyes are identified by a two-dimensional thin layer chromatographic method. An initial development in basic solution is followed by development in an acidic solution. The dye intermediates are identified by their color reactions with spray reagents and by their spatial relationships to known intermediates developed on the same plate. The chromatographic study included 30 dye intermediates proposed for use in commercial oxidation-type hair dyes.

scholarly journals Identification and Quantification of Phenolic Compounds in White Wines From the Apold Vineyard

2014 ◽  
Vol 6 (2) ◽  
pp. 65-67 ◽  
Author(s):  
Ecaterina Lengyel ◽  
Letiția Oprean
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Aromatic Compounds ◽  
White Wines ◽  
Chromatographic Methods ◽  
Wide Range ◽  
Identification And Quantification

Abstract Wines contain a wide range of aromatic compounds, each having its share in the characterization of the end product. This paper aims at highlighting the phenolic compounds that are present in three types of wine from the Apold Vineyard, compounds that have been determined by modern chromatographic methods. Anthocyanins, flavans, flavones and flavonoids, phenolic acids and microphenolic compounds have been determined in three wines: Pinot gris, Neuburger and Furmint. The results have led to the characterization of these wines and their classification according to the parameters acknowledged by the international authorities.

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