Simultaneous Multiselective Spectroelectrochemical Sensing of the Interaction between Protein and Its Ligand Using the Redox Dye Nile Blue as a Label

2008 ◽  
Vol 80 (24) ◽  
pp. 9642-9648 ◽  
Author(s):  
Hideki Kuramitz ◽  
Aigars Piruska ◽  
H. Brian Halsall ◽  
Carl J. Seliskar ◽  
William R. Heineman

Author(s):  
H. Chandrappa ◽  
Rajashekhar F. Bhajantri ◽  
B.K. Mahantesha ◽  
V. Ravindrachary ◽  
Shivaprasad Chalawadi


2020 ◽  
Vol 59 (35) ◽  
pp. 15152-15156 ◽  
Author(s):  
Beatriz Lozano‐Torres ◽  
Juan F. Blandez ◽  
Irene Galiana ◽  
Alba García‐Fernández ◽  
María Alfonso ◽  
...  


2021 ◽  
Author(s):  
Rui P. C. L. Sousa ◽  
João C. C. Ferreira ◽  
Maria João Sousa ◽  
M Sameiro Sameiro T T Gonçalves

The search for benzo[a]phenoxazines, Nile Blue derivatives, with high antifungal activity and cell labelling capacity based on our previously published works in this type of compounds, led us to the...



ACS Omega ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 2613-2625
Author(s):  
Mukti Mohammad ◽  
Harun Al Rasid Gazi ◽  
Kumud Pandav ◽  
Prateek Pandya ◽  
Md. Maidul Islam




2012 ◽  
Vol 66 (5) ◽  
pp. 1000-1006 ◽  
Author(s):  
Elisabeth Linton ◽  
Asif Rahman ◽  
Sridhar Viamajala ◽  
Ronald C. Sims ◽  
Charles D. Miller

In this study, a proton nuclear magnetic resonance (1H NMR) method was developed to quantitatively analyze polyhydroxyalkanoate (PHA) content in Cupriavidus necator H16, Azotobacter vinelandii AvOP, and mixed microbial cultures from the effluent of an agricultural waste treatment anaerobic digester. In contrast to previous methods, a single-step PHA extractive method using deuterated chloroform was established, thereby facilitating direct 1H NMR analysis. The accuracy of the method was verified through comparison with well-established gas chromatography (GC) methanolysis techniques. Nile blue fluorescence staining was also carried out to serve as an independent and qualitative indicator of intracellular PHA content. The results indicate that the 1H NMR method is appropriate for rapid and non-destructive quantification of overall PHA content and determination of PHA copolymer composition in a variety of cultures. Notably, this technique was effective in measuring PHA content in full-strength waste samples where high concentrations of background impurities and organic compounds are present. The straightforward procedures minimize error-introducing steps, require less time and materials, and result in an accurate method suitable for routine analyses.



2020 ◽  
Author(s):  
João Carlos Ferreira ◽  
Maria Sameiro Gonçalves ◽  
Rui Sousa ◽  
Maria Sousa


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