scholarly journals Improved Sensitivity of Ultralow Flow LC–MS-Based Proteomic Profiling of Limited Samples Using Monolithic Capillary Columns and FAIMS Technology

2020 ◽  
Vol 92 (21) ◽  
pp. 14702-14712
Author(s):  
Michal Greguš ◽  
James C. Kostas ◽  
Somak Ray ◽  
Susan E. Abbatiello ◽  
Alexander R. Ivanov
Keyword(s):  
Capillary Columns ◽  
Proteomic Profiling ◽  
Monolithic Capillary Columns

Silica-based monolithic capillary columns—Effect of preparation temperature on separation efficiency

2010 ◽  
Vol 1217 (36) ◽  
pp. 5737-5740 ◽  
Author(s):  
Josef Planeta ◽  
Dana Moravcová ◽  
Michal Roth ◽  
Pavel Karásek ◽  
Vladislav Kahle
Keyword(s):  
Separation Efficiency ◽  
Capillary Columns ◽  
Preparation Temperature ◽  
Monolithic Capillary Columns

scholarly journals Monolithic Silica Capillary Columns with Improved Retention and Selectivity for Amino Acids

Separations ◽  
2018 ◽  
Vol 5 (4) ◽  
pp. 48 ◽  
Author(s):  
Dana Moravcová ◽  
Josef Planeta
Keyword(s):  
Amino Acids ◽  
Ammonium Acetate ◽  
High Selectivity ◽  
Monolithic Column ◽  
Ammonium Hydroxide ◽  
Capillary Columns ◽  
Silica Monolith ◽  
Monolithic Capillary Columns ◽  
Modification Procedure ◽  
Silica Capillary

A strategy for the preparation of silica-based monolithic capillary columns (150 × 0.1 mm) with high selectivity to amino acids is presented. The zwitterionic columns were prepared by coating the silica monolith with [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide via 3-(trimethoxysilyl)propyl methacrylate. The columns were evaluated under isocratic conditions in hydrophilic interaction liquid chromatography. The best separation of amino acids was obtained on the monolithic column prepared by a stepwise modification procedure where the modification step was repeated four times. The mixture of fifteen amino acids was separated within 13 min using the mobile phase consisting of 75% acetonitrile and 25% 5 mmol/L ammonium acetate at pH 4.5.

Accurate molecular weight analysis of histones using FFE and RP-HPLC on monolithic capillary columns

2009 ◽  
Vol 32 (15-16) ◽  
pp. 2691-2698 ◽  
Author(s):  
Evert-Jan Sneekes ◽  
Jun Han ◽  
Monica Elliot ◽  
Juan Ausio ◽  
Remco Swart ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Capillary Columns ◽  
Rp Hplc ◽  
Monolithic Capillary Columns ◽  
Weight Analysis
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