Abstract
H5N1 is a highly pathogenic influenza virus that causes severe respiratory disease in birds and humans. The development of a highly sensitive detection method for H5H1 is vital to control spread and even terminate the outbreak of pandemic H5N1. Herein, we develop a highly sensitive local surface plasmon resonance (LSPR) sensor based on a gold-dot pattern chip for H5N1 detection. The LSPR sensor chip is fabricated by nano-imprint lithography, and it can detect and discriminate hemagglutinin H5 protein at femtogram-level sensitivity. Based on the results of the finite-difference time-domain simulation, the LSPR spectrum in the near-infrared region is suitable and appropriate to obtain the desired sensor sensitivity. The detection sensitivity of the sensor is 109 times higher than the immunochromatography method and is comparable to RT-PCR. Therefore, our sensor chip is very useful to provide valuable information for protection from pandemics.
Ultra-highly sensitive detection of influenza virus by Localized surface-plasmon resonance sensor
