DNA Polymerase II Supports the Replicative Bypass of N2-Alkyl-2′-deoxyguanosine Lesions in Escherichia coli Cells

2021 ◽  
Author(s):  
Yinan Wang ◽  
Jun Wu ◽  
Jiabin Wu ◽  
Yinsheng Wang
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Escherichia Coli Cells ◽  
Dna Polymerase Ii

Escherichia coli DNA polymerase II is homologous to α-like DNA polymerases

1991 ◽  
Vol 226-226 (1-2) ◽  
pp. 24-33 ◽  
Author(s):  
Hiroshi Iwasaki ◽  
Yoshizumi Ishino ◽  
Hiroyuki Toh ◽  
Atsuo Nakata ◽  
Hideo Shinagawa
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Dna Polymerase Ii

scholarly journals A Novel DNA Polymerase Family Found inArchaea

1998 ◽  
Vol 180 (8) ◽  
pp. 2232-2236 ◽  
Author(s):  
Yoshizumi Ishino ◽  
Kayoko Komori ◽  
Isaac K. O. Cann ◽  
Yosuke Koga
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Pyrococcus Furiosus ◽  
Dna Polymerases ◽  
Open Reading Frames ◽  
Gene Products ◽  
Polymerase Gene ◽  
Hyperthermophilic Archaeon ◽  
Dna Polymerase Ii ◽  
Reading Frames

ABSTRACT One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase-like open reading frames (ORFs) were found besides one ORF having the typical α-like DNA polymerase (family B). Recently, we identified the genes of DNA polymerase II (the second DNA polymerase) from the hyperthermophilic archaeonPyrococcus furiosus, which has also at least one α-like DNA polymerase (T. Uemori, Y. Sato, I. Kato, H. Doi, and Y. Ishino, Genes Cells 2:499–512, 1997). The genes in M. jannaschiiencoding the proteins that are homologous to the DNA polymerase II ofP. furiosus have been located and cloned. The gene products of M. jannaschii expressed in Escherichia colihad both DNA polymerizing and 3′→5′ exonuclease activities. We propose here a novel DNA polymerase family which is entirely different from other hitherto-described DNA polymerases.

scholarly journals DNA polymerase II as a fidelity factor in chromosomal DNA synthesis in Escherichia coli

2005 ◽  
Vol 58 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Magdalena Banach-Orlowska ◽  
Iwona J. Fijalkowska ◽  
Roel M. Schaaper ◽  
Piotr Jonczyk
Keyword(s):  
Escherichia Coli ◽  
Dna Synthesis ◽  
Dna Polymerase ◽  
Chromosomal Dna ◽  
Dna Polymerase Ii

scholarly journals The Escherichia coli polB gene, which encodes DNA polymerase II, is regulated by the SOS system.

1990 ◽  
Vol 172 (11) ◽  
pp. 6268-6273 ◽  
Author(s):  
H Iwasaki ◽  
A Nakata ◽  
G C Walker ◽  
H Shinagawa
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Sos System ◽  
Dna Polymerase Ii

Crystallization of DNA Polymerase II from Escherichia coli

1994 ◽  
Vol 238 (1) ◽  
pp. 120-122 ◽  
Author(s):  
Wayne F. Anderson ◽  
D.Bryan Prince ◽  
Hong Yu ◽  
Kevin McEntee ◽  
Myron F. Goodman
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Dna Polymerase Ii

scholarly journals Escherichia coli Cells with Increased Levels of DnaA and Deficient in Recombinational Repair Have Decreased Viability

2003 ◽  
Vol 185 (2) ◽  
pp. 630-644 ◽  
Author(s):  
Aline V. Grigorian ◽  
Rachel B. Lustig ◽  
Elena C. Guzmán ◽  
Joseph M. Mahaffy ◽  
Judith W. Zyskind
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Replication Fork ◽  
Replication Initiation ◽  
Recombinational Repair ◽  
Replication Forks ◽  
Dna Replication Initiation ◽  
Escherichia Coli Cells ◽  
Repair Pathways ◽  
Stalled Replication Forks

ABSTRACT The dnaA operon of Escherichia coli contains the genes dnaA, dnaN, and recF encoding DnaA, β clamp of DNA polymerase III holoenzyme, and RecF. When the DnaA concentration is raised, an increase in the number of DNA replication initiation events but a reduction in replication fork velocity occurs. Because DnaA is autoregulated, these results might be due to the inhibition of dnaN and recF expression. To test this, we examined the effects of increasing the intracellular concentrations of DnaA, β clamp, and RecF, together and separately, on initiation, the rate of fork movement, and cell viability. The increased expression of one or more of the dnaA operon proteins had detrimental effects on the cell, except in the case of RecF expression. A shorter C period was not observed with increased expression of the β clamp; in fact, many chromosomes did not complete replication in runout experiments. Increased expression of DnaA alone resulted in stalled replication forks, filamentation, and a decrease in viability. When the three proteins of the dnaA operon were simultaneously overexpressed, highly filamentous cells were observed (>50 μm) with extremely low viability and, in runout experiments, most chromosomes had not completed replication. The possibility that recombinational repair was responsible for the survival of cells overexpressing DnaA was tested by using mutants in different recombinational repair pathways. The absence of RecA, RecB, RecC, or the proteins in the RuvABC complex caused an additional ∼100-fold drop in viability in cells with increased levels of DnaA, indicating a requirement for recombinational repair in these cells.

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