Phase Separation Dynamics in Oil–Polyethylene Glycol–Sulfate–Water Based Three-Liquid-Phase Systems

2015 ◽  
Vol 54 (15) ◽  
pp. 3952-3960 ◽  
Author(s):  
Jieyuan Lin ◽  
Kun Huang ◽  
Zhicheng Suo ◽  
Xiaopei Li ◽  
Chuanxu Xiao ◽  
...  
Keyword(s):  
Phase Separation ◽  
Polyethylene Glycol ◽  
Liquid Phase ◽  
Water Based ◽  
Phase Systems

scholarly journals Taurine suppresses liquid-liquid phase separation of lysozyme protein

2021 ◽  
Author(s):  
Kanae Tsubotani ◽  
Sayuri Maeyama ◽  
Shigeru Murakami ◽  
Stephen W Schaffer ◽  
Takashi Ito
Keyword(s):  
Phase Separation ◽  
Polyethylene Glycol ◽  
Critical Temperature ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
Compatible Osmolyte ◽  
Hen Egg ◽  
Liquid Liquid Phase Separation ◽  
In Cells

AbstractTaurine is a compatible osmolyte that infers stability to proteins. Recent studies have revealed that liquid-liquid phase separation (LLPS) of proteins underlie the formation of membraneless organelles in cells. In the present study, we evaluated the role of taurine on LLPS of hen egg lysozyme. We demonstrated that taurine decreases the turbidity of the polyethylene glycol-induced crowding solution of lysozyme. We also demonstrated that taurine attenuates LLPS-dependent cloudiness of lysozyme solution with 0.5 or 1M NaCl at a critical temperature. Moreover, we observed that taurine inhibits LLPS formation of a heteroprotein mix solution of lysozyme and ovalbumin. These data indicate that taurine can modulate the formation of LLPS of proteins.

scholarly journals IMINATION OF CHITOSAN NANOFIBERS IN A HETEROGENEOUS SYSTEM. SYNTHESIS OPTIMIZATION AND IMPACT ON FIBER MORPHOLOGY

2021 ◽  
Vol 55 (7-8) ◽  
pp. 785-793
Author(s):  
ALEXANDRU ANISIEI ◽  
ANDRA-CRISTINA BOSTANARU ◽  
MIHAI MARES ◽  
LUMINITA MARIN
Keyword(s):  
Polyethylene Glycol ◽  
Liquid Phase ◽  
Polar Solvent ◽  
Solid Phase ◽  
Fiber Morphology ◽  
Glycol Chitosan ◽  
Fibrous Morphology ◽  
Phase Systems ◽  
The Impact ◽  
Heterogenous System

The paper aimed to prepare imino-chitosan fibers by the imination reaction in a heterogenous system, targeting the improvement of anti-pathogenic activity. To this end, porous neat chitosan fibers were prepared by electrospinning of the polyethylene glycol/chitosan blend, followed by polyethylene glycol removal. Imination of the neat chitosan fibers was carried out in three liquid phase systems using solvents of different polarity and, consequently, different ability to swell the solid phase chitosan fibers. The successful imination was qualitatively and quantitatively assessed by FTIR and 1H-NMR spectroscopy, and the impact of the liquid phase on the fibers’ morphology was evaluated by SEM, POM and AFM microscopy. Further, the antimicrobial activity of the imino-chitosan fibers was investigated on relevant bacterial and fungal strains. It was concluded that the prior swelling in water of the fibers improved the imination degree, while the use of a less polar solvent, such as toluene, favored the preservation of the fibrous morphology. The imination with an antimicrobial aldehyde endowed the chitosan fibers with the ability to create a physical barrier against pathogens.

Kinetics of phase separation for polyethylene glycol-phosphate two-phase systems

1995 ◽  
Vol 48 (3) ◽  
pp. 246-256 ◽  
Author(s):  
A. Kaul ◽  
R. A. M. Pereira ◽  
J. A. Asenjo ◽  
J. C. Merchuk
Keyword(s):  
Phase Separation ◽  
Polyethylene Glycol ◽  
Two Phase ◽  
Phase Systems ◽  
Kinetics Of

Liquid–liquid phase separation rescues the conformational stability of a DNA hairpin from pressure–stress

2019 ◽  
Vol 55 (72) ◽  
pp. 10673-10676 ◽  
Author(s):  
Loana Arns ◽  
Roland Winter
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Conformational Stability ◽  
Dna Hairpin ◽  
Two Phase ◽  
Dna Hairpins ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Pressure Stress ◽  
Liquid Liquid Phase Separation

Aqueous two-phase systems are able to rescue the conformational stability of DNA hairpins under harsh environmental conditions.

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