Use of Herbarium Voucher Specimens To Investigate Phytochemical Composition in Poisonous Plant Research

Author(s):  
Daniel Cook ◽  
Stephen T. Lee ◽  
Dale R. Gardner ◽  
Russell J. Molyneux ◽  
Robert L. Johnson ◽  
...  
Rangelands ◽  
2016 ◽  
Vol 38 (5) ◽  
pp. 241-249 ◽  
Author(s):  
James A. Pfister ◽  
Daniel Cook ◽  
Kip E. Panter ◽  
Kevin D. Welch ◽  
Lynn F. James

Author(s):  
K. E. Panter ◽  
L. F. James ◽  
T. L. Wierenga ◽  
C. C. Gay ◽  
E. S. Motteram ◽  
...  

2012 ◽  
Vol 8 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Kevin D. Welch ◽  
Kip E. Panter ◽  
Dale R. Gardner ◽  
Bryan L. Stegelmeier

2004 ◽  
Vol 52 (11) ◽  
pp. 3211-3230 ◽  
Author(s):  
Lynn F. James ◽  
Kip E. Panter ◽  
William Gaffield ◽  
Russell J. Molyneux

2019 ◽  
Vol 1 ◽  
pp. 58-66
Author(s):  
Harilal Upadhaya ◽  
Narayandatta Bastakoti

The study of Pteridophytic flora of Bhimkalipatan, Pokhara was conducted from May 2018 to October 2018. The Pteridophytic plants were collected and then dried for herbarium specimen preparation. The prepared Herbarium voucher specimens were identified based on standard literatures and finally verified from KATH, Kathmandu. A total of 27 species under 19 genera belonging to 11 families were recorded. The family Polypodiaceae was the largest family with eight species under five genera. The family Pteridaceae stood second with six species under three genera. The family Dryopteridaceae was third largest family represented by three species under three genera. In the same way, the families Lygodiaceae and Thelypteridaceae were represented by two species each under two genera respectively. The remaining six families i.e. Davalliaceae, Equisetaceae, Lycopodiaceae, Nephrolepidaceae, Selaginellaceae and Woodsiaceae were represented by one species each. The largest genera were Pteris and Pyrossia representing three species each followed by Adiantum, Drynaria, Lygodium and Thelypteris with two species each respectively and the remaining 13 genera are monotypic genera. Habitat wise, the collected species were terrestrial represented by nine species followed by seven epiphytes, two climbers and one lithophyte. Likewise, three species were found as terrestrial, epiphytes and lithophytes; three species as epiphytes and lithophytes and two species as terrestrial and lithophytes.


2009 ◽  
pp. 361-376 ◽  
Author(s):  
L. F. James ◽  
K. E. Panter ◽  
W. Gaffield ◽  
R. J. Molyneux ◽  
J. Weinzweig

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jianping Han ◽  
Yingjie Zhu ◽  
Xiaochen Chen ◽  
Baoshen Liao ◽  
Hui Yao ◽  
...  

An ideal DNA barcoding region should be short enough to be amplified from degraded DNA. In this paper, we discuss the possibility of using a short nuclear DNA sequence as a barcode to identify a wide range of medicinal plant species. First, the PCR and sequencing success rates of ITS and ITS2 were evaluated based entirely on materials from dry medicinal product and herbarium voucher specimens, including some samples collected back to 90 years ago. The results showed that ITS2 could recover 91% while ITS could recover only 23% efficiency of PCR and sequencing by using one pair of primer. Second, 12861 ITS and ITS2 plant sequences were used to compare the identification efficiency of the two regions. Four identification criteria (BLAST, inter- and intradivergence Wilcoxon signed rank tests, and TaxonDNA) were evaluated. Our results supported the hypothesis that ITS2 can be used as a minibarcode to effectively identify species in a wide variety of specimens and medicinal materials.


Author(s):  
J. Sepulveda-Saavedra ◽  
I. Vander-Klei ◽  
M. Venhuis ◽  
Y. Piñeyro-Lopez

Karwinskia humboldtiana is a poisonous plant that grows in semi desertic areas in north and central México. It produces several substances with different toxic effects. One of them designated T-514 damages severely the lung, kidney and liver, producing in the hepatoeyte large intracellular fat deposits and necrosis. Preliminary observations demonstrated that three is a decrease in the amount of peroxisomes in the hepatocytes of experimentally intoxicated rats and monkeys. To study the effect exerted by the T-514 on peroxisomes, a yeast model was selected, thus, three species: Saccha romices cerevisiae, Ilansenula polymorpha and Candida boidinii were used, because there is information concerning their peroxisome's morphology, enzyme content, biological behaviour under different culture conditions and biogenesis.


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