scholarly journals The Short ITS2 Sequence Serves as an Efficient Taxonomic Sequence Tag in Comparison with the Full-Length ITS

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jianping Han ◽  
Yingjie Zhu ◽  
Xiaochen Chen ◽  
Baoshen Liao ◽  
Hui Yao ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Nuclear Dna ◽  
Its2 Sequence ◽  
Degraded Dna ◽  
Success Rates ◽  
Rank Tests ◽  
Signed Rank ◽  
Wide Range ◽  
Voucher Specimens ◽  
Herbarium Voucher

An ideal DNA barcoding region should be short enough to be amplified from degraded DNA. In this paper, we discuss the possibility of using a short nuclear DNA sequence as a barcode to identify a wide range of medicinal plant species. First, the PCR and sequencing success rates of ITS and ITS2 were evaluated based entirely on materials from dry medicinal product and herbarium voucher specimens, including some samples collected back to 90 years ago. The results showed that ITS2 could recover 91% while ITS could recover only 23% efficiency of PCR and sequencing by using one pair of primer. Second, 12861 ITS and ITS2 plant sequences were used to compare the identification efficiency of the two regions. Four identification criteria (BLAST, inter- and intradivergence Wilcoxon signed rank tests, and TaxonDNA) were evaluated. Our results supported the hypothesis that ITS2 can be used as a minibarcode to effectively identify species in a wide variety of specimens and medicinal materials.

scholarly journals Seed germination requirements of Hypericum scruglii, an endangered medicinal plant species of Sardinia (Italy)

Botany ◽  
2020 ◽  
Vol 98 (10) ◽  
pp. 615-621 ◽  
Author(s):  
Marco Porceddu ◽  
Martina Sanna ◽  
Sara Serra ◽  
Maria Manconi ◽  
Gianluigi Bacchetta
Keyword(s):  
Medicinal Plant ◽  
Seed Bank ◽  
Seed Viability ◽  
Base Temperature ◽  
Endemic Plant ◽  
Endangered Medicinal Plant ◽  
High Viability ◽  
Wide Range ◽  
Baseline Information ◽  
Germination Requirements

Hypericum scruglii Bacch., Brullo & Salmeri is an endangered endemic plant of Sardinia, and the phloroglucinol compounds identified in this species have been reported to inhibit the activity of the Human Immunodeficiency Virus. Seed banks are a genetic repository that effectively preserve taxa of conservation interest, and they hold knowledge about the biology and germination ecophysiology of the taxa they preserve. The main goals of this study were to investigate the germination requirements for H. scruglii, to evaluate the seed viability after eight years of long-term conservation and to suggest an efficient protocol for germination. Seeds stored at –25 °C in the seed bank were tested at temperatures from 5 to 30 °C and 25/10 °C. Base temperature (Tb) and thermal time (θ50) for germination were estimated. Seeds of H. scruglii germinated over a wide range of temperatures, responding positively to high temperatures and 25/10 °C. Tb was estimated at 4.92 °C, and θ50 was estimated at 198.27 degree days. The high viability detected in seeds of H. scruglii allows us to suggest the use of seeds stored in a seed bank when fresh material for plant propagation is scarce. Our results provide new and useful baseline information for implementing conservation and multiplication strategies for this endangered medicinal plant.

scholarly journals Endangered Uyghur Medicinal PlantFerulaIdentification through the Second Internal Transcribed Spacer

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Congzhao Fan ◽  
Xiaojin Li ◽  
Jun Zhu ◽  
Jingyuan Song ◽  
Hui Yao
Keyword(s):  
Medicinal Plant ◽  
Related Species ◽  
Internal Transcribed Spacer ◽  
Morphological Characteristics ◽  
Its2 Sequence ◽  
Sequence Length ◽  
Local Alignment ◽  
Accurate Identification ◽  
Distance Method ◽  
Closely Related Species

The medicinal plantFerulahas been widely used in Asian medicine, especially in Uyghur medicine in Xinjiang, China. Given that various substitutes and closely related species have similar morphological characteristics,Ferulais difficult to distinguish based on morphology alone, thereby causing confusion and threatening the safe use ofFerula. In this study, internal transcribed spacer 2 (ITS2) sequences were analyzed and assessed for the accurate identification of two salableFerulaspecies (Ferula sinkiangensisandFerula fukangensis) and eight substitutes or closely related species. Results showed that the sequence length of ITS2 ranged from 451 bp to 45 bp, whereas guanine and cytosine contents (GC) were from 53.6% to 56.2%. A total of 77 variation sites were detected, including 63 base mutations and 14 insertion/deletion mutations. The ITS2 sequence correctly identified 100% of the samples at the species level using the basic local alignment search tool 1 and nearest-distance method. Furthermore, neighbor-joining tree successfully identified the genuine plantsF. sinkiangensisandF. fukangensisfrom their succedaneum and closely related species. These results indicated that ITS2 sequence could be used as a valuable barcode to distinguish Uyghur medicineFerulafrom counterfeits and closely related species. This study may broaden DNA barcoding application in the Uyghur medicinal plant field.

Review on pharmacological and phytochemical prospects of traditional medicinal plant: Persicaria hydropiper (Smartweed)

2021 ◽  
Vol 21 ◽  
Author(s):  
Abdul Nasir ◽  
Atif Ali Khan Khalil ◽  
Muhammad Zeeshan Bhatti ◽  
Ashfaq Ur Rehman ◽  
Jiayi Li ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Traditional Medicine ◽  
Northern Hemisphere ◽  
Biological Activities ◽  
Folk Medicine ◽  
Present Review ◽  
Polygonum Hydropiper ◽  
Wide Range ◽  
Pharmacological Data ◽  
Different Parts

: Persicaria hydropiper (L.) Delarbre (family Polygonacea), commonly known as Polygonum hydropiper, is a popular medicinal plant used in traditional medicine. The plant is indigenous to the tropical northern hemisphere and temperate zone including China, Bangladesh, India, and Japan. The plant is used in folk medicine for numerous ailments such as hemorrhoids, antifertility, diarrhea, and dyspepsia. Its medicinal usage in Unani, Ayurveda, Siddha, and other traditional medicine is well-recognized. So far, a wide range of active phytochemicals of this plant has been identified, such as flavonoids, sulphated flavonoids, terpenoids, anthraquinones, steroids, coumarin, simple phenolics, and others. Pharmacological data reported in the literature suggest that various parts of P. hydropiper exhibit antimicrobial, antioxidant, hypoglycemic, antidepressant, cardioprotective, hepatoprotective, anticancer, and antifertility effects. The present review aim is to compile the coherently document research on the phytochemical, pharmacological, and biological activities of P. hydropiper from different parts of the globe.

scholarly journals PENGARUH LAMAZE EXERCISE TERHADAP SKALA NYERI MENSTRUASI (DYSMENORRHOEA) PADA REMAJA PUTRI DI SMA BUDI MULIA JAKARTA

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Veronica Yeni Rahmawati
Keyword(s):  
Rating Scale ◽  
Numeric Rating Scale ◽  
Rank Test ◽  
Menstrual Pain ◽  
Significance Level ◽  
Signed Rank ◽  
Wide Range ◽  
Post Test ◽  
Signed Rank Test ◽  
Pharmacological Method

Menstrual pain present as cramps (ranging from mild to severe), which may disturb dailyactivities. Lamaze exercise is non pharmacological method which use breathingrelaxation. This research was to analyze Lamaze exercise to reduction of menstrual pain.This study used pra experimental pre post test design. The population was the studentswith menstrual pain at Budi Mulia Senior High School Jakarta. The sampling techniquewas purposive sampling. Thirty one students participated in this study. The independentvariable was Lamaze exercise and the dependent variable was menstrual pain.Instrument which used to measure pain was Visual Analog Numeric Rating Scale. Datawere analyzed with Wilcoxon Signed Rank Test with significance α ≤ 0.005. The resultshowed that Lamaze exercise decreasd menstrual pain. The result of statistical testshowed significance level p = 0.000 and the deviation between pre and post mean is2.32, which means that there was decreasing menstrual pain in student in pre test andpost test. It can be concluded that Lamaze exercise can influence menstrual pain indecrease respondent of the research. Lamaze exercise can provide a relaxing effect.Further experiment with different respondent characteristic to obtain more wide range ofresult.Keywords: menstrual pain, Lamaze, relaxing effect, non pharmacological method

scholarly journals An open label single arm prospective clinical study on Vatagajankusharasa with Pippali Churna and Manjishta Kwatha as Anupana in Viswachi (cervical spondylosis)

2020 ◽  
Vol 5 (01) ◽  
pp. 73-80
Author(s):  
Moh Gulfam ◽  
Totad Muttappa ◽  
Neelam Bisht ◽  
Vishnu M L ◽  
Yadu Gopan
Keyword(s):  
Clinical Study ◽  
Ordinal Data ◽  
Cervical Spondylosis ◽  
Signs And Symptoms ◽  
Upper Extremities ◽  
Secondary Outcome ◽  
Prospective Clinical Study ◽  
Rank Tests ◽  
Open Label ◽  
Signed Rank

Background: Viswachi is one among the 80 Nanatmaja Vata Vyadhi. This disease affects the neck and upper extremities with the signs and symptoms like Ruk, Stambha, Toda, Bahu Karmakshaya. Vatagajankusha Rasa is a combination of Vyosha, Bhasmas, Vatsanabha, Karkatasringi, Haritaki etc. It has Vatakaphahara, Vikasi, Vyavayi, Rasayana etc. properties. It is said to be effective in treating Visawachi in 7 days if given along with Pippali Churna and Manjishta Kwatha. Aims and Objectives: To evaluate the efficacy of Vatagajankusha Rasa with Pippali Churna and Manjishta Kwatha as Anupana in the management of Viswachi (Cervical spondylosis). Methodology: Among 35 registered patients, 30 completed the course of treatment. They were administered with Vatagajankusha Rasa 1 tablet (125 mg) after food with Anupana 3gm Pippali Churna and 15 ml Manjishta Kwatha twice daily (morning and evening) for a period of 7 days. Nominal and ordinal data were analysed using non parametric tests like McNemar and Wilcoxon’s signed rank tests respectively. Result: Assessment parameters like Ruk, Toda, Sthambha and Bahukarma Kshya. There was statistically significant improvement in the primary and secondary outcome measures (p less than 0.05 was observed). Conclusion: Vatagajankusha Rasa with Pippali Churna and Manjishta Kwatha as Anupana is effective in the management of Viswachi (Cervical spondylosis).

scholarly journals Лексико-семантический анализ лексемы балтырған (с использованием материалов корпуса башкирского языка)

2020 ◽  
Vol 13 (5) ◽  
pp. 1406-1414
Author(s):  
Anita Sh. Ishmukhametova ◽  
Keyword(s):  
Comparative Analysis ◽  
Medicinal Plant ◽  
Cultural Heritage ◽  
Plant Species ◽  
Comparative Investigation ◽  
Medicinal Herb ◽  
Wide Range ◽  
Plant Names ◽  
The World

Identification of names of plant curatives and substances in folk and fiction texts shows close interactions between man and the world, attitudes of people towards nature. Research in phytonyms and medicinal plant names proper is most essential for the understanding of a nation’s cultural heritage. The paper examines the lexeme балтырған in Bashkir discourse. Materials. The analyzed materials include linguistic dictionaries, folklore and fiction texts of the Machine Fund of the Bashkir Language, and etymological dictionaries of Altaic languages. Goals. The study aims at a comparative investigation of the lexeme балтырған ‘hogweed’. Results. The term proves a widespread phytonym in Bashkir discourse, which is attested by that it denotes a wide range of plant species in Bashkir and has parallels in other Turkic and Mongolic languages. The lexeme is included in academic, explanatory, dialectal, phrasal, and mythological dictionaries of the Bashkir language. The comparative analysis shows that baltyrγan ‘hogweed’ usually denotes a plant of the order Apiales, a medicinal herb. Baltyrγan~ baltirγana contains the initial bal / baltïr / baldïr with the meaning ‘green, young, fresh’.

scholarly journals Mediatieve behandeling bij mensen met zowel neuro­cognitieve stoornissen als persoonlijkheids­problematiek

2020 ◽  
Keyword(s):  
Behavioral Interventions ◽  
Cognitive Model ◽  
Rank Tests ◽  
Signed Rank

Uitgangspunt van deze studie is dat comorbide maladaptieve persoonlijkheidstrekken (CMPT) van invloed zijn op gedragsmatige en psychologische symptomen van dementie (BPSD). Het doel van dit onderzoek is het opzetten en exploreren van de haalbaarheid van Cognitive Model for Behavioral Interventions (CoMBI). Veertig patiënten met BPSD en CMPT van twee ouderenpsychiatrische afdelingen werden met CoMBI behandeld. De haalbaarheid werd beoordeeld middels de patiëntenstroom, en naleving en aanvaardbaarheid van de behandeling door familieleden en psychiatrisch verpleegkundigen. CMPT werd in kaart gebracht met informantenvragenlijsten. Verandering in BPSD werd gemeten via voor- en nametingen. Om de verschillen tussen de meetmomenten te bepalen, werden Wilcoxon signed rank-tests uitgevoerd en effectgroottes berekend. Van de 312 opgenomen patiënten kwamen 138 patiënten in aanmerking voor inclusie. 64 (46,4%) patiënten gingen voor of kort na de voormeting met ontslag. Bij 28 (20,3%) patiënten kon CoMBI niet toegepast worden. Uiteindelijk werden veertig (29,0%) patiënten geïncludeerd voor de analyse. Wilcoxon signed rank-tests toonden een significante afname van BPSD met middelgrote (r=0,45) tot grote (r=0,56) effectgroottes. CoMBI blijkt een toepasbaar en werkzaam behandelmodel voor probleemgedrag bij patiënten met BPSD en CMPT. Het behandelmodel is geassocieerd met een significante afname van probleemgedrag ongeacht de etiologie ervan.

scholarly journals Age and anthropometric variables and success in mixed martial arts

2016 ◽  
Author(s):  
Christopher Kirk
Keyword(s):  
Martial Arts ◽  
Bayes Factor ◽  
Individual Performance ◽  
Chronological Age ◽  
Competitive Sport ◽  
Rank Tests ◽  
Full Cohort ◽  
Paired Samples ◽  
Signed Rank ◽  
The Relationship

Anthropometry and chronological age has been demonstrated to have an effect on individual performance in competitive sport, with the relationship between stature and wingspan being found to be selective criteria in many sports, although evidence for this in MMA is negligible. In this study, n = 278 professional MMA bouts were analysed with the winners and losers being compared in terms of chronological age, stature, wingspan, stature-to-wingspan ratio (S:W) and method of win/loss using paired samples t tests, Wilcoxon signed-rank tests, one way ANOVAs, Kruskal-Wallis tests (all ≤ .05) and Bayes Factor (BF 10 ). The results showed that for the most part anthropometric differences have no effect on who wins the bout, but taller bout losers are most likely to lose via strikes. Across the full cohort and several competitive divisions, it was found that older participants are significantly more likely to lose, and are also significantly more likely to lose via strikes. Participants who won via decision were found to be significantly older than those who won via strikes or submission.

A class of sequential grouped signed rank tests

1985 ◽  
Vol 4 (3) ◽  
pp. 153-177
Author(s):  
Marion R. Reynolds ◽  
Saad T. Bakir
Keyword(s):  
Rank Tests ◽  
Signed Rank
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