Development of a Singlet Oxygen Absorption Capacity (SOAC) Assay Method. Measurements of the SOAC Values for Carotenoids and α-Tocopherol in an Aqueous Triton X-100 Micellar Solution

2017 ◽  
Vol 65 (4) ◽  
pp. 784-792 ◽  
Author(s):  
Kazuo Mukai ◽  
Aya Ouchi ◽  
Nagao Azuma ◽  
Shingo Takahashi ◽  
Koichi Aizawa ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Micellar Solution ◽  
Absorption Capacity ◽  
Assay Method ◽  
Oxygen Absorption ◽  
Triton X

Development of Singlet Oxygen Absorption Capacity (SOAC) Assay Method. 2. Measurements of the SOAC Values for Carotenoids and Food Extracts

2011 ◽  
Vol 59 (8) ◽  
pp. 3717-3729 ◽  
Author(s):  
Koichi Aizawa ◽  
Yuko Iwasaki ◽  
Aya Ouchi ◽  
Takahiro Inakuma ◽  
Shin-ichi Nagaoka ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Absorption Capacity ◽  
Assay Method ◽  
Oxygen Absorption

Development of Singlet Oxygen Absorption Capacity (SOAC) Assay Method Using a Microplate Reader

2016 ◽  
Vol 99 (1) ◽  
pp. 193-197 ◽  
Author(s):  
Shingo Takahashi ◽  
Yuko Iwasaki-Kino ◽  
Koichi Aizawa ◽  
Junji Terao ◽  
Kazuo Mukai
Keyword(s):  
Singlet Oxygen ◽  
Temperature Control ◽  
Control Unit ◽  
Natural Antioxidants ◽  
Absorption Capacity ◽  
Assay Method ◽  
Oxygen Absorption ◽  
Temperature Control Unit ◽  
Microplate Reader ◽  
Good Agreement

Abstract Recently, a new assay method that can quantify the singlet oxygen absorption capacity (SOAC) of natural antioxidants and food extracts was developed. The SOAC values were measured in ethanol–chloroform–D2O (50 + 50 + 1, v/v/v) solution at 35°C using a UV-Vis spectrophotometer equipped with a six-channel cell positioner and an electron-temperature control unit. In the present study, measurement of the SOAC values was performed for eight representative carotenoids and three vegetable extracts (tomato, carrot, and red paprika) using a versatile instrument, the microplate reader. A 24-well glass microplate was used for measurements because a plastic microplate, commonly used in the laboratory, dissolves in the ethanol–chloroform–D2O solution. The SOAC values of eight carotenoids and three vegetable extracts measured using a microplate reader were in good agreement with the corresponding values measured using a UV-Vis spectrophotometer, suggesting that the microplate reader is an applicable instrument for the measurement of reliable SOAC values for general antioxidants and food extracts in solution.

Development of Singlet Oxygen Absorption Capacity (SOAC) Assay Method. 3. Measurements of the SOAC Values for Phenolic Antioxidants

2012 ◽  
Vol 60 (32) ◽  
pp. 7905-7916 ◽  
Author(s):  
Kazuo Mukai ◽  
Aya Ouchi ◽  
Shingo Takahashi ◽  
Koichi Aizawa ◽  
Takahiro Inakuma ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Absorption Capacity ◽  
Assay Method ◽  
Oxygen Absorption ◽  
Phenolic Antioxidants

scholarly journals Proposal of a Micro Analysis for Singlet Oxygen Absorption Capacity using a Disposable 96-Well Microplate

2018 ◽  
Vol 14 (4) ◽  
pp. 126-130
Author(s):  
Yu Wang ◽  
Tomomi Kimura ◽  
Tomoko Nohara ◽  
Jufang Shen ◽  
Hajime Hatta
Keyword(s):  
Singlet Oxygen ◽  
Absorption Capacity ◽  
Oxygen Absorption ◽  
Micro Analysis
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