Synthesis and Evaluation of Halogenated 5-(2-Hydroxyphenyl)pyrazoles as Pseudilin Analogues Targeting the Enzyme IspD in the Methylerythritol Phosphate Pathway

2020 ◽  
Vol 68 (10) ◽  
pp. 3071-3078
Author(s):  
Jili Wang ◽  
Yaqing Zhou ◽  
Xiuwen Wang ◽  
Lixia Duan ◽  
Jiang Duan ◽  
...  
Keyword(s):  
Methylerythritol Phosphate Pathway ◽  
Methylerythritol Phosphate

Corrigendum to “Biosynthesis of the diterpenoid steviol, an ent-kaurene derivative from Stevia rebaudiana Bertoni, via the methylerythritol phosphate pathway”

2000 ◽  
Vol 41 (39) ◽  
pp. 7595 ◽  
Author(s):  
Nicole Totté ◽  
Lionel Charon ◽  
Michel Rohmer ◽  
Frans Compernolle ◽  
Ilse Baboeuf ◽  
...  
Keyword(s):  
Stevia Rebaudiana ◽  
Methylerythritol Phosphate Pathway ◽  
Methylerythritol Phosphate ◽  
Stevia Rebaudiana Bertoni

Hyperaccumulation of fucoxanthin by enhancing methylerythritol phosphate pathway in Phaeodactylum tricornutum

2021 ◽  
Author(s):  
Ting-Bin Hao ◽  
Yang Lu ◽  
Zhong-Hong Zhang ◽  
Si-Fen Liu ◽  
Xiang Wang ◽  
...  
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Methylerythritol Phosphate Pathway ◽  
Methylerythritol Phosphate

Elicitor induced activation of the methylerythritol phosphate pathway toward phytoalexins biosynthesis in rice

2007 ◽  
Vol 65 (1-2) ◽  
pp. 177-187 ◽  
Author(s):  
Atsushi Okada ◽  
Takafumi Shimizu ◽  
Kazunori Okada ◽  
Tomohisa Kuzuyama ◽  
Jinichiro Koga ◽  
...  
Keyword(s):  
Methylerythritol Phosphate Pathway ◽  
Methylerythritol Phosphate

scholarly journals The mevalonate pathway but not the methylerythritol phosphate pathway is critical for elaioplast and pollen coat development in Arabidopsis

2018 ◽  
Vol 35 (4) ◽  
pp. 381-385 ◽  
Author(s):  
Keiko Kobayashi ◽  
Masashi Suzuki ◽  
Toshiya Muranaka ◽  
Noriko Nagata
Keyword(s):  
Mevalonate Pathway ◽  
Methylerythritol Phosphate Pathway ◽  
Methylerythritol Phosphate ◽  
Pollen Coat

Methylerythritol Phosphate Pathway

2010 ◽  
pp. 560-590
Author(s):  
Michel Rohmer
Keyword(s):  
Methylerythritol Phosphate Pathway ◽  
Methylerythritol Phosphate

Diversity in isoprene unit biosynthesis: The methylerythritol phosphate pathway in bacteria and plastids

2007 ◽  
Vol 79 (4) ◽  
pp. 739-751 ◽  
Author(s):  
Michel Rohmer
Keyword(s):  
Mep Pathway ◽  
Reduction Step ◽  
Methylerythritol Phosphate Pathway ◽  
Isoprene Unit ◽  
Methylerythritol Phosphate ◽  
Selection Of

The long-overlooked methylerythritol phosphate (MEP) pathway represents an alternative to the mevalonate route for the formation of isoprene units. It is found in most bacteria as well as in the plastids of all phototrophic organisms. A selection of significant steps of its discovery and elucidation are presented in this contribution, as well as a complete hypothetical biogenetic scheme for the last reduction step.

scholarly journals Modulating the Precursor and Terpene Synthase Supply for the Whole-Cell Biocatalytic Production of the Sesquiterpene (+)-Zizaene in a Pathway Engineered E. coli

Genes ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 478 ◽  
Author(s):  
Francisco Aguilar ◽  
Thomas Scheper ◽  
Sascha Beutel
Keyword(s):  
Essential Oil ◽  
Metabolic Flux ◽  
Mevalonate Pathway ◽  
Terpene Synthase ◽  
Methylerythritol Phosphate Pathway ◽  
Main Compound ◽  
E Coli ◽  
Methylerythritol Phosphate ◽  
Cosmetic Industry ◽  
Multiple Copies

The vetiver essential oil from Chrysopogon zizanioides contains fragrant sesquiterpenes used widely in the formulation of nearly 20% of men’s cosmetics. The growing demand and issues in the supply have raised interest in the microbial production of the sesquiterpene khusimol, the main compound of the vetiver essential oil due to its woody smell. In this study, we engineered the biosynthetic pathway for the production of (+)-zizaene, the immediate precursor of khusimol. A systematic approach of metabolic engineering in Escherichia coli was applied to modulate the critical bottlenecks of the metabolic flux towards (+)-zizaene. Initially, production of (+)-zizaene was possible with the endogenous methylerythritol phosphate pathway and the codon-optimized zizaene synthase (ZS). Raising the precursor E,E-farnesyl diphosphate supply through the mevalonate pathway improved the (+)-zizaene titers 2.7-fold, although a limitation of the ZS supply was observed. To increase the ZS supply, distinct promoters were tested for the expression of the ZS gene, which augmented 7.2-fold in the (+)-zizaene titers. Final metabolic enhancement for the ZS supply by using a multi-plasmid strain harboring multiple copies of the ZS gene improved the (+)-zizaene titers 1.3-fold. The optimization of the fermentation conditions increased the (+)-zizaene titers 2.2-fold, achieving the highest (+)-zizaene titer of 25.09 mg L−1. This study provides an alternative strategy to enhance the terpene synthase supply for the engineering of isoprenoids. Moreover, it demonstrates the development of a novel microbial platform for the sustainable production of fragrant molecules for the cosmetic industry.

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