Association Equilibrium for Cross-Associating Chains in a Good Solvent: Crowding and Other Nonideality Effects

2016 ◽  
Vol 120 (29) ◽  
pp. 7234-7243 ◽  
Author(s):  
Igor Yu. Gotlib ◽  
Ivan K. Malov ◽  
Alexey I. Victorov ◽  
Mikhail A. Voznesenskiy
Keyword(s):  
Association Equilibrium

scholarly journals The C Terminus of the Ribosomal-Associated Protein LrtA is an Intrinsically Disordered Oligomer

2018 ◽  
Vol 19 (12) ◽  
pp. 3902 ◽  
Author(s):  
José L. Neira ◽  
A. Marcela Giudici ◽  
Felipe Hornos ◽  
Arantxa Arbe ◽  
Bruno Rizzuti
Keyword(s):  
Computational Modelling ◽  
Terminal Region ◽  
Physiological Conditions ◽  
Intrinsically Disordered ◽  
Disordered Protein ◽  
C Terminus ◽  
Conformational Preferences ◽  
Self Association ◽  
Association Equilibrium ◽  
Post Stress

The 191-residue-long LrtA protein of Synechocystis sp. PCC 6803 is involved in post-stress survival and in stabilizing 70S ribosomal particles. It belongs to the hibernating promoting factor (HPF) family, intervening in protein synthesis. The protein consists of two domains: The N-terminal region (N-LrtA, residues 1101), which is common to all the members of the HPF, and seems to be well-folded; and the C-terminal region (C-LrtA, residues 102-191), which is hypothesized to be disordered. In this work, we studied the conformational preferences of isolated C-LrtA in solution. The protein was disordered, as shown by computational modelling, 1D-1H NMR, steady-state far- UV circular dichroism (CD) and chemical and thermal denaturations followed by fluorescence and far-UV CD. Moreover, at physiological conditions, as indicated by several biochemical and hydrodynamic techniques, isolated C-LrtA intervened in a self-association equilibrium, involving several oligomerization reactions. Thus, C-LrtA was an oligomeric disordered protein.

Simulating Calculation of the Dissociation-Association Equilibrium of Dimer and Monomer of Trimethylaluminum in Gas Phase

2006 ◽  
Vol 22 (11) ◽  
pp. 1393-1398
Author(s):  
WU Shu-Ying ◽  
◽  
◽  
YANG Li-Xin ◽  
SONG Chun-Xia ◽  
...  
Keyword(s):  
Gas Phase ◽  
Simulating Calculation ◽  
Association Equilibrium

scholarly journals Solution Properties of Phycocyanin. IV. Studies of the Self-association Equilibrium of Phycocyanin in a pH 6.8 Solution

1977 ◽  
Vol 50 (11) ◽  
pp. 2892-2895 ◽  
Author(s):  
Naomichi Iso ◽  
Haruo Mizuno ◽  
Takahide Saito ◽  
Noriko Nitta ◽  
Katsuaki Yoshizaki
Keyword(s):  
The Self ◽  
Solution Properties ◽  
Self Association ◽  
Association Equilibrium

Infrared Characterization and Determination of Self-Association Equilibrium Constants for Methacrylic Acid Copolymers

2000 ◽  
Vol 39 (2) ◽  
pp. 197-223 ◽  
Author(s):  
CHRISTOPHER S. CLEVELAND ◽  
STEPHEN P. FEARNLEY ◽  
YUHONG HU ◽  
MARK E. WAGMAN ◽  
PAUL C. PAINTER ◽  
...  
Keyword(s):  
Methacrylic Acid ◽  
Equilibrium Constants ◽  
Methacrylic Acid Copolymers ◽  
Self Association ◽  
Association Equilibrium

The formation of (CH3)7Si2+ in (CH3)4Si/CH4 mixtures and CH3− exchange reactions between (CH3)4Si, (CH3)4Ge, and (CH3)4Sn studied by high pressure mass spectrometry

1987 ◽  
Vol 65 (12) ◽  
pp. 2849-2854 ◽  
Author(s):  
Anastasia C. M. Wojtyniak ◽  
Xiaoping Li ◽  
John A. Stone
Keyword(s):  
Mass Spectrometry ◽  
High Pressure ◽  
Equilibrium Constant ◽  
Mass Spectrometer ◽  
Excellent Agreement ◽  
Ion Source ◽  
Methyl Groups ◽  
Exchange Reactions ◽  
Equilibrium Reactions ◽  
Association Equilibrium

The association equilibrium [Formula: see text] has been studied in a high pressure mass spectrometer ion source using tetramethylsilane/methane mixtures. Measurement of the equilibrium constant over a range of temperatures yields ΔH0 = −22.3 ± 0.4 kcal mol−1 and ΔS0 = −35.2 ± 0.9 cal mol−1 K−1. Collision-assisted dissociation experiments suggest that the methyl groups retain their integrity in (CH3)7Si2+. Mixed ions such as (CH3)7SiGe+ and (CH3)7GeSn+ were not observed in mixtures of (CH3)4X and (CH3)4Y(X ≠ Y = Si, Ge, Sn). Instead CH3− transfer equilibrium reactions were observed viz. [Formula: see text] (ΔH0 = −10.2 ± 1.2 kcal mol−1, ΔS0 = −3.7 ± 2.4 cal K−1 mol−1) and [Formula: see text], ΔS0 = −0.9 ± 1.6 cal K−1 mol−1. These are in excellent agreement with some published differences in appearance potentials for (CH3)3X+ from (CH3)4X (X = Si, Ge, Sn).

Ion-ion-solvent interactions in solution. I. Solutions of LiClO4 in acetone

1982 ◽  
Vol 35 (9) ◽  
pp. 1775 ◽  
Author(s):  
DW James ◽  
RE Mayes
Keyword(s):  
Raman Band ◽  
Ion Pairs ◽  
Ion Association ◽  
Solvent Interactions ◽  
Solvation Numbers ◽  
Solvated Ions ◽  
Component Band ◽  
Li Ion ◽  
Contact Ion Pairs ◽  
Association Equilibrium

Vibrational spectra and 7Li, 13C and 35Cl n.m.r. spectra have been obtained for solutions of LiClO4 in acetone for salt concentrations from 0.05 to 6 M. Infrared spectra give qualitative indications of ion association. Analysis of the Raman band due to C-C stretching in acetone yields solvation numbers for the Li+ ion of the order of 3. Component band analysis of the ClO4- symmetric stretching vibrational band and the various n.m.r. spectra lead to the identification of solvent-separated ion pairs, contact ion pairs and ion aggregates, in addition to free solvated ions. The dependence on salt concentration of all four species has been determined. The association quotient for the association equilibrium (Li+)s(ClO4)- ↔ [Li+(acetone)ClO4-)s was determined to be 1.4 � 0.3 dm3 mol-1.

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