scholarly journals Perturbation of Short Hydrogen Bonds in Photoactive Yellow Protein via Noncanonical Amino Acid Incorporation

2019 ◽  
Vol 123 (23) ◽  
pp. 4844-4849 ◽  
Author(s):  
Benjamin Thomson ◽  
Johan Both ◽  
Yufan Wu ◽  
Robert M. Parrish ◽  
Todd J. Martínez ◽  
...  
Keyword(s):  
Amino Acid ◽  
Hydrogen Bonds ◽  
Photoactive Yellow Protein ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Noncanonical Amino Acid ◽  
Short Hydrogen Bonds

scholarly journals Reporter system architecture affects measurements of noncanonical amino acid incorporation efficiency and fidelity

2020 ◽  
Vol 5 (2) ◽  
pp. 573-588
Author(s):  
Potts K. A. ◽  
Stieglitz J. T. ◽  
Lei M. ◽  
Van Deventer J. A.
Keyword(s):  
Amino Acid ◽  
System Architecture ◽  
Reporter System ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Chemical Functionality ◽  
Reporter Systems ◽  
Noncanonical Amino Acid ◽  
Incorporation Efficiency

Quantitative reporter systems are critical tools for engineering cells to synthesize proteins containing diverse chemical functionality.

Performance of optimized noncanonical amino acid mutagenesis systems in the absence of release factor 1

2016 ◽  
Vol 12 (6) ◽  
pp. 1746-1749 ◽  
Author(s):  
Yunan Zheng ◽  
Marc J. Lajoie ◽  
James S. Italia ◽  
Melissa A. Chin ◽  
George M. Church ◽  
...  
Keyword(s):  
Amino Acid ◽  
Release Factor ◽  
Amino Acid Incorporation ◽  
E Coli ◽  
Acid Incorporation ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

Unconditional deletion of RF1 in a genomically recoded E. coli enables multisite noncanonical amino acid incorporation by UAG suppression.

scholarly journals A Robust and Quantitative Reporter System To Evaluate Noncanonical Amino Acid Incorporation in Yeast

2018 ◽  
Vol 7 (9) ◽  
pp. 2256-2269 ◽  
Author(s):  
Jessica T. Stieglitz ◽  
Haixing P. Kehoe ◽  
Ming Lei ◽  
James A. Van Deventer
Keyword(s):  
Amino Acid ◽  
Reporter System ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Noncanonical Amino Acid
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