Performance of optimized noncanonical amino acid mutagenesis systems in the absence of release factor 1

2016 ◽  
Vol 12 (6) ◽  
pp. 1746-1749 ◽  
Author(s):  
Yunan Zheng ◽  
Marc J. Lajoie ◽  
James S. Italia ◽  
Melissa A. Chin ◽  
George M. Church ◽  
...  
Keyword(s):  
Amino Acid ◽  
Release Factor ◽  
Amino Acid Incorporation ◽  
E Coli ◽  
Acid Incorporation ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

Unconditional deletion of RF1 in a genomically recoded E. coli enables multisite noncanonical amino acid incorporation by UAG suppression.

scholarly journals Release Factor Inhibiting Antimicrobial Peptides Improve Nonstandard Amino Acid Incorporation in Wild-type Bacterial Cells

2020 ◽  
Vol 15 (7) ◽  
pp. 1852-1861 ◽  
Author(s):  
Erkin Kuru ◽  
Rosa-Maria Määttälä ◽  
Karen Noguera ◽  
Devon A. Stork ◽  
Kamesh Narasimhan ◽  
...  
Keyword(s):  
Amino Acid ◽  
Antimicrobial Peptides ◽  
Bacterial Cells ◽  
Release Factor ◽  
Wild Type ◽  
Amino Acid Incorporation ◽  
Acid Incorporation

scholarly journals Perturbation of Short Hydrogen Bonds in Photoactive Yellow Protein via Noncanonical Amino Acid Incorporation

2019 ◽  
Vol 123 (23) ◽  
pp. 4844-4849 ◽  
Author(s):  
Benjamin Thomson ◽  
Johan Both ◽  
Yufan Wu ◽  
Robert M. Parrish ◽  
Todd J. Martínez ◽  
...  
Keyword(s):  
Amino Acid ◽  
Hydrogen Bonds ◽  
Photoactive Yellow Protein ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Noncanonical Amino Acid ◽  
Short Hydrogen Bonds

scholarly journals Reporter system architecture affects measurements of noncanonical amino acid incorporation efficiency and fidelity

2020 ◽  
Vol 5 (2) ◽  
pp. 573-588
Author(s):  
Potts K. A. ◽  
Stieglitz J. T. ◽  
Lei M. ◽  
Van Deventer J. A.
Keyword(s):  
Amino Acid ◽  
System Architecture ◽  
Reporter System ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Chemical Functionality ◽  
Reporter Systems ◽  
Noncanonical Amino Acid ◽  
Incorporation Efficiency

Quantitative reporter systems are critical tools for engineering cells to synthesize proteins containing diverse chemical functionality.

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