scholarly journals Matrix-Matched Calibration Curves for Assessing Analytical Figures of Merit in Quantitative Proteomics

2020 ◽  
Vol 19 (3) ◽  
pp. 1147-1153 ◽  
Author(s):  
Lindsay K. Pino ◽  
Brian C. Searle ◽  
Han-Yin Yang ◽  
Andrew N. Hoofnagle ◽  
William S. Noble ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Figures Of Merit ◽  
Calibration Curves ◽  
Analytical Figures Of Merit

scholarly journals Matrix-matched calibration curves for assessing analytical figures of merit in quantitative proteomics

2019 ◽  
Author(s):  
Lindsay K Pino ◽  
Han-Yin Yang ◽  
William Stafford Noble ◽  
Brian C Searle ◽  
Andrew N Hoofnagle ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Quantitative Proteomics ◽  
Protein Abundance ◽  
Complex Samples ◽  
Figures Of Merit ◽  
Data Independent Acquisition ◽  
Proteomics Experiment ◽  
Series Of Experiments ◽  
Analytical Figures Of Merit

AbstractMass spectrometry is a powerful tool for quantifying protein abundance in complex samples. Advances in sample preparation and the development of data independent acquisition (DIA) mass spectrometry approaches have increased the number of peptides and proteins measured per sample. Here we present a series of experiments demonstrating how to assess whether a peptide measurement is quantitative by mass spectrometry. Our results demonstrate that increasing the number of detected peptides in a proteomics experiment does not necessarily result in increased numbers of peptides that can be measured quantitatively.

Room-Temperature Luminescence of a Variety of Compounds on 1% α-Cyclodextrin/NaCl Mixture

1989 ◽  
Vol 43 (5) ◽  
pp. 810-812 ◽  
Author(s):  
Marsha D. Richmond ◽  
Robert J. Hurtubise
Keyword(s):  
Solid Surface ◽  
Room Temperature ◽  
Lower Cost ◽  
Model Compounds ◽  
Figures Of Merit ◽  
Room Temperature Luminescence ◽  
Analytical Figures Of Merit

With the use of model compounds of widely different functionality, it was found that a 1% α-cyclodextrin/NaCl mixture could be used in place of an 80% α-cyclodextrin/NaCl mixture to obtain solid surface room-temperature fluorescence (RTF) and phosphorescence (RTP) data. Analytical figures of merit are reported for four compounds. The use of a smaller percentage of α-cyclodextrin results in lower cost; no loss of analytical integrity, compared with that for an 80% α-cyclodextrin/NaCl mixture; and easier handling of the α-cyclodextrin/NaCl mixtures.

scholarly journals DEVELOPMENT OF FLOW INJECTION METHOD FOR ONLINE DETERMINATION OF THIOCYANATE BASED ON OXIDATION BY PERMANGANATE

2010 ◽  
Vol 10 (2) ◽  
pp. 167-171 ◽  
Author(s):  
Hermin Sulistyarti ◽  
Spas D. Kolev ◽  
Stephanie Lim
Keyword(s):  
Detection Limit ◽  
Thyroid Gland ◽  
Flow Injection ◽  
Hydrogen Cyanide ◽  
Figures Of Merit ◽  
Sample Throughput ◽  
Teflon Membrane ◽  
Developing Method ◽  
Analytical Figures Of Merit

The importance of developing method for thiocyanate becomes obvious, because thiocyanate can inhibit iodine uptake of thyroid gland leading to mumps disease. In this work, thiocyanate is oxidized by permanganate in the acid donor stream to cyanide, which is directly converted to hydrogen cyanide. Then, hydrogen cyanide diffuses through a Teflon membrane into acceptor stream containing nickel(II) in ammoniacal buffer to form tetracyanonickelate(II) which is detected spectrophotometrically at 267 nm. Analytical figures of merit were linear up to 50 mg L-1 for thiocyanate, with RSD of 1.34%, and detection limit of 0.07 mg L-1, respectively. Interfering anions were eliminated under stoichiometric amount of permanganate and sample throughput was 20 h-1. The method was validated for determining thiocyanate samples from synthetic and gold process waters with satisfactory results.   Keywords: Thiocyanate, flow injection, permanganate, spectrophotometry

Analytical Figures of Merit for Multisensor Arrays

ACS Sensors ◽  
2020 ◽  
Vol 5 (2) ◽  
pp. 580-587 ◽  
Author(s):  
Hadi Parastar ◽  
Dmitry Kirsanov
Keyword(s):  
Figures Of Merit ◽  
Analytical Figures Of Merit
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