Growth of Myelin Figures from Parent Multilamellar Vesicles

Abstract A child with congenital neutropenia was studied using bone marrow culture and ultrastructural and cytochemical techniques. The patient's marrow cells formed a large number of granulocytic colonies of normal size in culture, and her peripheral blood leukocytes produced adequate colony-stimulating factor. No serum inhibitors were identified. The patient's promyelocytes from direct marrow and culture appeared normal in ultrastructure, and primary granules, contained peroxidase and acid phosphatase activity. Myelocytes and rare segmented neutrophils from direct marrow specimens demonstrated atypical notched nuclei, myelin figures in Golgi lamellae and primary (azurophilic) granules, and no identifiable secondary (specific) granules. These data indicate an intrinsic neutrophil defect which allows normal proliferation of precursor cells, but results in abnormal granulogenesis and apparent inability to form secondary granules.

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Cross-sectional view of myelin figures

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/0005-2736(89)90110-7 ◽

1989 ◽

Vol 985 (1) ◽

pp. 101-105 ◽

Cited By ~ 32

Author(s):

I. Sakurai ◽

T. Suzuki ◽

S. Sakurai

Keyword(s):

Cross Sectional ◽

Myelin Figures

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Confocal mapping of myelin figures with micro-Raman spectroscopy

Applied Physics A ◽

10.1007/s00339-017-1450-z ◽

2017 ◽

Vol 124 (1) ◽

Cited By ~ 1

Author(s):

Jung-Ren Huang ◽

Yu-Che Cheng ◽

Hung Ji Huang ◽

Hai-Pang Chiang

Keyword(s):

Raman Spectroscopy ◽

Micro Raman Spectroscopy ◽

Myelin Figures

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CELLULAR INJURY IN VITRO: PHASE CONTRAST STUDIES ON INJURED CYTOPLASM

The Journal of Cell Biology ◽

10.1083/jcb.14.3.401 ◽

1962 ◽

Vol 14 (3) ◽

pp. 401-420 ◽

Cited By ~ 11

Author(s):

I. K. Buckley

Keyword(s):

Endoplasmic Reticulum ◽

Enzyme Inhibitors ◽

Acinar Cells ◽

Carcinoma Cells ◽

Secretion Granules ◽

Fluid Collections ◽

Walker Carcinoma ◽

Myelin Figure ◽

Myelin Figures

Unfixed, compressed acinar cells of rat pancreas, isolated by mechanical and enzymatic means, were examined by phase microscopy and photomicrographed using 35 mm film and electronic flash illumination. Similarly, observations were made on Walker carcinoma cells; in addition, these cells were treated with solutions containing either phosphatidase A or enzyme inhibitors. Acinar cells contained, besides nuclei, perinuclear droplets and secretion granules, various membranous and vacuolar structures. The basal cytoplasm showed parallel dark lines interpreted as endoplasmic reticulum. In some cells, fragmentation of the reticulum was followed by the direct incorporation of fragments into simple myelin figures. In other cells it appeared that phase-lucent linear structures and vacuoles were derived by dilatation of cisternae of the endoplasmic reticulum. Perinuclear fluid collections arose either by dilation of the perinuclear cisternae of the endoplasmic reticulum or by fluid dilatation of the nuclear envelope. Phosphatidase A disrupted early vacuoles of Walker carcinoma cells. From this and the direct involvement of elements of the endoplasmic reticulum in myelin figures, it was concluded that the membranes limiting the endoplasmic reticulum incorporate phosphatides in continuous layers. While many severely injured cells formed large vacuoles, others developed concentrically laminated myelin figures; it was concluded that both types of structure derived from phosphatides liberated intracellularly, the vacuoles by vesicular myelin figure formation.

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Liposome percutaneous penetration in vivo

Toxicology Research and Application ◽

10.1177/2397847317723196 ◽

2017 ◽

Vol 1 ◽

pp. 239784731772319 ◽

Cited By ~ 4

Author(s):

A Lymberopoulos ◽

C Demopoulou ◽

M Kyriazi ◽

MS Katsarou ◽

N Demertzis ◽

...

Keyword(s):

Stratum Corneum ◽

Rhodamine B ◽

Transdermal Delivery ◽

Penetration Enhancers ◽

Percutaneous Penetration ◽

Hairless Mice ◽

Liposomal Form ◽

Multilamellar Vesicles ◽

Small Unilamellar Vesicles

Objectives: Liposomes are reported as penetration enhancers for dermal and transdermal delivery. However, little is known about their percutaneous penetration and as to at which level they deliver encapsulated drugs. The penetration of multilamellar vesicles (MLVs) and small unilamellar vesicles (SUVs), in comparison to one of their lipid components, was investigated. Methods: Using the fluorescent lipid, Lissamine Rhodamine B-PE (R), as a constituent, MLV and SUV liposomes were prepared, tested, and R, MLV, or SUV were applied in vivo on the back of hairless mice. Absorption of each was evaluated at the levels of stratum corneum, living skin, and blood by fluorometry. Results: Penetration of the lipid R in stratum corneum in the nonliposomal form exceeded that in the liposomal form and only R penetrates the living skin in a statistically significant manner. No statistical significant absorption into blood was observed with either form. Conclusions: Liposomes size did not play an important role in penetration to stratum corneum. The lipid constituent in the nonliposomal form penetrated at higher rates into stratum corneum and living skin. Even though these liposomes entered stratum corneum, they were not significantly absorbed into viable skin or blood.

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