Amplification of Antibody Responses to Antigen Using Small Multilamellar Vesicles for Delivery System

The function of belayed vesicles as productive transporters for drugs, immunizations, indicative specialists, and other bioactive operators has prompted a fast headway in the liposomal drug conveyance system. The pharmacy- elements and pharmacokinetics properties are altered for the liposomal delivery system,which on thewholeleads to an increased the rapeutic index with decreased toxicity. The liposome can be named multilamellar vesicles or unilamellar vesicles, which can be additionally named large unilamellar vesicles (LUV) or small unilamellar vesicles (SUV). The part of liposome as a medication conveyance framework is to convey drug in a controlled way, diminishing unfortunate reactions improving it is in vitro and in vivo action, just as lessening the harmfulness of the medication and upgrading the viability of the exemplified drug. This article gives a review of techniques to the planning of liposome, just as diagnostic strategies for control physical, concoction, and organic boundaries for various kinds of medications.

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The immunostimulating complex (ISCOM) is an efficient mucosal delivery system for respiratory syncytial virus (RSV) envelope antigens inducing high local and systemic antibody responses

Clinical & Experimental Immunology ◽

10.1046/j.1365-2249.1998.00650.x ◽

1998 ◽

Vol 113 (2) ◽

pp. 235-243 ◽

Cited By ~ 60

Author(s):

HU ◽

Elvander ◽

Merza ◽

Åkerblom ◽

Brandenburg ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Delivery System ◽

Antibody Responses ◽

Mucosal Delivery ◽

Syncytial Virus

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Iscom is an efficient mucosal delivery system for subsp. (MmmSC) antigens inducing high mucosal and systemic antibody responses

FEMS Immunology & Medical Microbiology ◽

10.1016/s0928-8244(98)00111-4 ◽

1999 ◽

Vol 23 (1) ◽

pp. 5-12 ◽

Cited By ~ 1

Author(s):

I ABUSUGRA ◽

B MOREIN

Keyword(s):

Delivery System ◽

Antibody Responses ◽

Mucosal Delivery

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Iscom is an efficient mucosal delivery system forMycoplasma mycoidessubsp.mycoides(MmmSC) antigens inducing high mucosal and systemic antibody responses

FEMS Immunology & Medical Microbiology ◽

10.1111/j.1574-695x.1999.tb01710.x ◽

1999 ◽

Vol 23 (1) ◽

pp. 5-12 ◽

Cited By ~ 1

Author(s):

Izzeldin Abusugra ◽

Bror Morein

Keyword(s):

Delivery System ◽

Antibody Responses ◽

Mucosal Delivery

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Oral Immunization with Attenuated Salmonella enterica Serovar Typhimurium Encoding Cryptosporidium parvum Cp23 and Cp40 Antigens Induces a Specific Immune Response in Mice

Clinical and Vaccine Immunology ◽

10.1128/cvi.00089-09 ◽

2009 ◽

Vol 16 (9) ◽

pp. 1272-1278 ◽

Cited By ~ 32

Author(s):

Alvaro J. Benitez ◽

Nina McNair ◽

Jan R. Mead

Keyword(s):

Vaccine Strain ◽

Delivery System ◽

Cryptosporidium Parvum ◽

Salmonella Enterica ◽

Plasmid Stability ◽

Oral Immunization ◽

Antibody Responses ◽

Vector System ◽

Enzyme Linked Immunosorbent Assay ◽

Serovar Typhimurium

ABSTRACT Attenuated Salmonella enterica serovar Typhimurium vaccine strain SL3261 was used as an antigen delivery system for the oral immunization of mice against two Cryptosporidium parvum antigens, Cp23 and Cp40. Each antigen was subcloned into the pTECH1 vector system, which allows them to be expressed as fusion proteins with highly immunogenic fragment C of tetanus toxin under the control of the anaerobically inducible nirB promoter. The recombinant vector was introduced into Salmonella Typhimurium vaccine strain SL3261, and the stable soluble expression of the chimeric protein was evaluated and confirmed by Western blotting with polyclonal C. parvum antisera. Mice were inoculated orally with a single dose of SL3261/pTECH-Cp23 or Cp40, respectively, and plasmid stability was demonstrated both in vitro and in vivo. Specific serum immunoglobulin G (IgG) antibodies against the Cp23 or Cp40 antigen were detected by enzyme-linked immunosorbent assay 35 days after immunization. Also, serum IgA and mucosal (feces) IgA antibodies were detected in 30% of the mice immunized with Cp23. In addition, prime-boosting with Cp23 and Cp40 DNA vaccine vectors followed by Salmonella immunization significantly increased antibody responses to both antigens. Our data show that a single oral inoculation with recombinant S. Typhimurium SL3261 can induce specific antibody responses to the Cp23 or Cp40 antigen from C. parvum in mice, suggesting that recombinant Salmonella is a feasible delivery system for a vaccine against C. parvum infection.

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