scholarly journals Selective Intracellular Delivery of Recombinant Arginine Deiminase (ADI) Using pH-Sensitive Cell Penetrating Peptides To Overcome ADI Resistance in Hypoxic Breast Cancer Cells

2015 ◽  
Vol 13 (1) ◽  
pp. 262-271 ◽  
Author(s):  
Tzyy-Harn Yeh ◽  
Yun-Ru Chen ◽  
Szu-Ying Chen ◽  
Wei-Chiang Shen ◽  
David K. Ann ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Intracellular Delivery ◽  
Cell Penetrating Peptides ◽  
Ph Sensitive ◽  
Arginine Deiminase ◽  
Sensitive Cell ◽  
Cell Penetrating

Targeted Intracellular Delivery of Trastuzumab Using Designer Phage Lambda Nanoparticles Alters Cellular Programs in Human Breast Cancer Cells

ACS Nano ◽  
2021 ◽  
Author(s):  
Alexis Catala ◽  
Monika Dzieciatkowska ◽  
Guankui Wang ◽  
Arthur Gutierrez-Hartmann ◽  
Dmitri Simberg ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Intracellular Delivery ◽  
Human Breast Cancer Cells ◽  
Phage Lambda ◽  
Human Breast

Engineered Histidine-Enriched Facial Lipopeptides for Enhanced Intracellular Delivery of Functional siRNA to Triple Negative Breast Cancer Cells

2019 ◽  
Vol 11 (5) ◽  
pp. 4719-4736 ◽  
Author(s):  
Abhijit Biswas ◽  
Kasturee Chakraborty ◽  
Chiranjit Dutta ◽  
Sanchita Mukherjee ◽  
Paramita Gayen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Intracellular Delivery

scholarly journals Reversible activation of pH-sensitive cell penetrating peptides attached to gold surfaces

2015 ◽  
Vol 51 (2) ◽  
pp. 273-275 ◽  
Author(s):  
Joe E. Baio ◽  
Denise Schach ◽  
Adrian V. Fuchs ◽  
Lars Schmüser ◽  
Nils Billecke ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Cell Penetrating Peptides ◽  
Ph Sensitive ◽  
Sensitive Cell ◽  
Gold Surfaces ◽  
Transition Mechanism ◽  
Coil Transition ◽  
Cell Penetrating ◽  
Covalently Bound

Here, we demonstrate that a fusion protein mimic, designed to achieve a reversible, pH-driven helix-coil transition mechanism, retain its functionality when covalently bound to a surface.

Construction and evaluation of pH-sensitive immunoliposomes for enhanced delivery of anticancer drug to ErbB2 over-expressing breast cancer cells

2017 ◽  
Vol 13 (3) ◽  
pp. 1219-1227 ◽  
Author(s):  
Tianshu Li ◽  
Takuya Amari ◽  
Kentaro Semba ◽  
Tadashi Yamamoto ◽  
Shinji Takeoka
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Anticancer Drug ◽  
Breast Cancer Cells ◽  
Ph Sensitive

scholarly journals Cell-penetrating doxorubicin released from Elastin-like polypeptide kills doxorubicin-resistant cancer cells in vitro study

2020 ◽  
Author(s):  
Jung Su Ryu ◽  
Felix Kratz ◽  
Drazen Raucher
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
In Vitro Study ◽  
Fold Increase ◽  
Membrane Penetration ◽  
Cancer Cell Death ◽  
Cell Penetrating ◽  
Resistant Cells

Abstract Background: Elastin-like polypeptide (ELP) undergoes its characteristic of phase transitioning in response to ambient temperature. ELP therefore has been be used as a thermosensitive vector for the delivery of chemotherapy agents since it can be targeted to hyperthermic tumors. This novel strategy introduces unprecedented options for treating cancer, with fewer concerns about side effects. In this study, the ELP system was further modified with an enzyme-cleavable linker in order to release drugs within tumors. This system consists of ELP, a matrix metalloproteinase (MMP) substrate, a cell penetrating peptide (CPP), and 6-maleimidocaproyl amide derivative of doxorubicin (Dox). This construct may be initially targeted to the tumor by application of mild heat after administration. Within the hyperthermic tumor, then this construct is cleaved by MMP, releasing CPP-Dox, which can infiltrate tumor tissues and penetrate cell membranes.Methods: We produced the construct in E.coli and examined its cleavage by MMP enzymes in vitro. Flow cytometry and confocal analysis were used to verify the facilitated uptake of the digested cell-penetrating Dox by breast cancer cells and Dox-resistant cells. Cytotoxicity tests further demonstrated improvements in bioavailability of cell-penetrating Dox following the enhanced cellular uptake of the cancer cells. Comparisons with the non-cleavable ELP counterpart were paralleled.Results: This strategy shows up to a 4-fold increase in cell penetration and results in more death in breast cancer cells than the ELP-Dox. Even in doxorubicin-resistant cells (NCI/ADR and MES/ADR), ELP-released, cell-penetrating doxorubicin demonstrated better membrane penetration, leading to at least twice the killing of resistant cells than ELP-Dox and free Dox. Conclusion: MMP-digested CPP-Dox shows better membrane penetration and induces more cancer cell death in vitro. This CPP-complexed Dox released from ELP kills even dox-resistant cells more efficiently than both free doxorubicin and non-cleaved ELP-CPP-Dox.

Targeted phase-change contrast agents for intracellular delivery to breast cancer tumors: An In vitro study in MDA-MB-231 breast cancer cells

2016 ◽  
Vol 140 (4) ◽  
pp. 3371-3371
Author(s):  
Kyle P. Hadinger ◽  
Joseph P. Marshalek ◽  
Paul S. Sheeran ◽  
Pier Ingram ◽  
Russell S. Witte ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Phase Change ◽  
Contrast Agents ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
In Vitro Study ◽  
Intracellular Delivery ◽  
Vitro Study
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