scholarly journals Reversible activation of pH-sensitive cell penetrating peptides attached to gold surfaces

2015 ◽  
Vol 51 (2) ◽  
pp. 273-275 ◽  
Author(s):  
Joe E. Baio ◽  
Denise Schach ◽  
Adrian V. Fuchs ◽  
Lars Schmüser ◽  
Nils Billecke ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Cell Penetrating Peptides ◽  
Ph Sensitive ◽  
Sensitive Cell ◽  
Gold Surfaces ◽  
Transition Mechanism ◽  
Coil Transition ◽  
Cell Penetrating ◽  
Covalently Bound

Here, we demonstrate that a fusion protein mimic, designed to achieve a reversible, pH-driven helix-coil transition mechanism, retain its functionality when covalently bound to a surface.

scholarly journals Cell Penetrating Peptides Mediated DNA-Free Transfection in Arabidopsis and Chinese Cabbage

2021 ◽  
Author(s):  
Han Wu ◽  
Zhipeng Zhang ◽  
Kai Zhu ◽  
Fulai Ke ◽  
Fei Zhang ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Genome Editing ◽  
Chinese Cabbage ◽  
Dna Sequences ◽  
Room Temperature ◽  
Genetically Modified Organisms ◽  
Transfection Efficiency ◽  
Cell Penetrating Peptides ◽  
Root Tips ◽  
Cell Penetrating

Abstract Background: The third-generation genome editing system CRISPR/Cas had shown strong application prospects in crop genetic improvement. However, this technology largely depends on genetic transformation. Public concerns on GMO (genetically modified organisms) safety, as well as related regulations, have restricted the application. Therefore, establishing DNA-free transfection system is important to promote CRISPR/Cas genome editing in agriculture.Results：In this paper, cell penetrating peptides fusion protein (CPP-mCherry) was found to be effective on DNA-free transfection. DNA sequences of nine tandem arginine (R9), one cysteine (cys), reporter mCherry and histidine label were sequentially constructed into pET 45B+ expression vector and transformed into Escherichia coli BL21(DE3) strain. CPP-mCherry fusion protein can be induced by 1mM IPTG for at least 1 hour in 28 °C. CPP-mCherry fusion protein can be obtained by 200W ultrasonication, then purified by Ni column and MWCO dialysis. The Arabidopsis thaliana root tips and leaves, as well as Chinese cabbage microspores and 3-week-old microspore embryo can be used as transfected recipient. Concentration can be selected between 10-100μg/ml and incubated overnight at room temperature. R9-cys-mCherry protein can be translocated into the nucleus of microspore. The transfection efficiency of root tips reached 100% and of microspore and MDE was 8.13% and 94.79%, respectively. Conclusions: Here, a CPP mediated DNA-free transfection system was built in dicots. These results lay a technical foundation of DNA-free genome editing.

scholarly journals Monitoring the cytosolic entry of cell-penetrating peptides using a pH-sensitive fluorophore

2015 ◽  
Vol 51 (11) ◽  
pp. 2162-2165 ◽  
Author(s):  
Ziqing Qian ◽  
Patrick G. Dougherty ◽  
Dehua Pei
Keyword(s):  
Cell Penetrating Peptides ◽  
Ph Sensitive ◽  
Cytosolic Delivery ◽  
Cell Penetrating

A simple and effective method for monitoring the cytosolic delivery of cell-penetrating peptides.

scholarly journals Polymeric micelles functionalized with cell penetrating peptides as potential pH-sensitive platforms in drug delivery for cancer therapy: A review

2021 ◽  
pp. 103264
Author(s):  
Suliman Khan ◽  
Yasaman Vahdani ◽  
Arif Hussain ◽  
Setareh Haghighat ◽  
Fatemeh Heidari ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cancer Therapy ◽  
Polymeric Micelles ◽  
Cell Penetrating Peptides ◽  
Ph Sensitive ◽  
Cell Penetrating
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