Multiphoton Fabrication of Fibronectin-Functionalized Protein Micropatterns: Stiffness-Induced Maturation of Cell–Matrix Adhesions in Human Mesenchymal Stem Cells

2017 ◽  
Vol 9 (35) ◽  
pp. 29469-29480 ◽  
Author(s):  
Jiaoni Ma ◽  
Chuenwai Li ◽  
Nan Huang ◽  
Xinna Wang ◽  
Minghui Tong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Cell Matrix ◽  
Multiphoton Fabrication

scholarly journals IL-1β mediated nanoscale surface clustering of integrin α5β1 regulates the adhesion of mesenchymal stem cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Stephanie A. Maynard ◽  
Ekaterina Pchelintseva ◽  
Limor Zwi-Dantsis ◽  
Anika Nagelkerke ◽  
Sahana Gopal ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Spatial Organization ◽  
Human Mesenchymal Stem Cells ◽  
Super Resolution ◽  
Integrin Α5β1 ◽  
Cell Matrix ◽  
Cluster Area ◽  
Rapid Clearance ◽  
Resolution Imaging

AbstractClinical use of human mesenchymal stem cells (hMSCs) is limited due to their rapid clearance, reducing their therapeutic efficacy. The inflammatory cytokine IL-1β activates hMSCs and is known to enhance their engraftment. Consequently, understanding the molecular mechanism of this inflammation-triggered adhesion is of great clinical interest to improving hMSC retention at sites of tissue damage. Integrins are cell–matrix adhesion receptors, and clustering of integrins at the nanoscale underlies cell adhesion. Here, we found that IL-1β enhances adhesion of hMSCs via increased focal adhesion contacts in an α5β1 integrin-specific manner. Further, through quantitative super-resolution imaging we elucidated that IL-1β specifically increases nanoscale integrin α5β1 availability and clustering at the plasma membrane, whilst conserving cluster area. Taken together, these results demonstrate that hMSC adhesion via IL-1β stimulation is partly regulated through integrin α5β1 spatial organization at the cell surface. These results provide new insight into integrin clustering in inflammation and provide a rational basis for design of therapies directed at improving hMSC engraftment.

Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm

2010 ◽  
Vol 30 (6) ◽  
pp. 455-455 ◽  
Author(s):  
Dongyan Shi ◽  
Dan Ma ◽  
Feiqing Dong ◽  
Chen Zong ◽  
Liyue Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

The Effect of Link N on the Differentiation of Human Mesenchymal Stem Cells

2012 ◽  
Vol 2 (1_suppl) ◽  
pp. s-0032-1320001-s-0032-1320001
Author(s):  
F. Mwale ◽  
H. T. Wang ◽  
L. Haglund ◽  
P. J. Roughley ◽  
J. Antoniou
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

Generation of human mesenchymal stem cells lacking diabetoporosity-associated miRNAs using CRISPR/Cas9

2020 ◽  
Author(s):  
I Foessl ◽  
A Groselj-Strele ◽  
JC Piswanger-Sölkner ◽  
H Dobnig ◽  
A Fahrleitner-Pammer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells
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