scholarly journals Inhibition of the GDP-d-Mannose Dehydrogenase from Pseudomonas aeruginosa Using Targeted Sugar Nucleotide Probes

2020 ◽  
Vol 15 (12) ◽  
pp. 3086-3092
Author(s):  
Laura Beswick ◽  
Eleni Dimitriou ◽  
Sanaz Ahmadipour ◽  
Ayesha Zafar ◽  
Martin Rejzek ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sugar Nucleotide

scholarly journals Targeted Chemoenzymatic Synthesis of Sugar Nucleotide Probes Reveal an Inhibitor of the GDP-D-Mannose Dehydrogenase from Pseudomonas Aeruginosa

2020 ◽  
Author(s):  
Laura Beswick ◽  
Eleni Dimitriou ◽  
Sanaz Ahmadipour ◽  
Ayesha Zafar ◽  
Martin Rejzek ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Autosomal Recessive ◽  
Genetic Disorder ◽  
Loss Of Function ◽  
Bacterial Strains ◽  
Sugar Nucleotide ◽  
Guanosine Diphosphate ◽  
Mannuronic Acid ◽  
Mucoid Conversion ◽  
Lung Infections

<p>Sufferers of the autosomal recessive genetic disorder cystic fibrosis are at extremely high risk for contracting chronic lung infections. Over their lifetime one bacterial strain in particular, <i>Pseudomonas aeruginosa</i>, becomes the dominant pathogen. Bacterial strains incur loss-of-function mutations in the mucA gene that lead to a phenomenon known as mucoid conversion, resulting in copious secretion of alginate, a carbohydrate exopolysaccharide. Strategies that can stop the production of alginate in mucoid <i>Pseudomonas aeruginosa </i>infections are therefore of paramount importance. To aid in this we developed a series of sugar nucleotide chemical tools to probe an enzyme critical to alginate biosynthesis, guanosine diphosphate mannose dehydrogenase (GMD). This enzyme catalyses the irreversible formation of the alginate sugar nucleotide building block, guanosine diphosphate mannuronic acid. Using a chemoenzymatic strategy we accessed a series of modified sugar nucleotides, identifying a C6-amide derivative of the native substrate as a micromolar inhibitor of GMD.<b> </b>This discovery will provide a framework for wider inhibition strategies against GMD to be developed.<b></b></p>

scholarly journals Targeted Chemoenzymatic Synthesis of Sugar Nucleotide Probes Reveal an Inhibitor of the GDP-D-Mannose Dehydrogenase from Pseudomonas Aeruginosa

2020 ◽  
Author(s):  
Laura Beswick ◽  
Eleni Dimitriou ◽  
Sanaz Ahmadipour ◽  
Ayesha Zafar ◽  
Martin Rejzek ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Autosomal Recessive ◽  
Genetic Disorder ◽  
Loss Of Function ◽  
Bacterial Strains ◽  
Sugar Nucleotide ◽  
Guanosine Diphosphate ◽  
Mannuronic Acid ◽  
Mucoid Conversion ◽  
Lung Infections

<p>Sufferers of the autosomal recessive genetic disorder cystic fibrosis are at extremely high risk for contracting chronic lung infections. Over their lifetime one bacterial strain in particular, <i>Pseudomonas aeruginosa</i>, becomes the dominant pathogen. Bacterial strains incur loss-of-function mutations in the mucA gene that lead to a phenomenon known as mucoid conversion, resulting in copious secretion of alginate, a carbohydrate exopolysaccharide. Strategies that can stop the production of alginate in mucoid <i>Pseudomonas aeruginosa </i>infections are therefore of paramount importance. To aid in this we developed a series of sugar nucleotide chemical tools to probe an enzyme critical to alginate biosynthesis, guanosine diphosphate mannose dehydrogenase (GMD). This enzyme catalyses the irreversible formation of the alginate sugar nucleotide building block, guanosine diphosphate mannuronic acid. Using a chemoenzymatic strategy we accessed a series of modified sugar nucleotides, identifying a C6-amide derivative of the native substrate as a micromolar inhibitor of GMD.<b> </b>This discovery will provide a framework for wider inhibition strategies against GMD to be developed.<b></b></p>

Multiple erythematous nodules as a manifestation of Pseudomonas aeruginosa septicemia

1980 ◽  
Vol 116 (4) ◽  
pp. 446-447 ◽  
Author(s):  
D. Schlossberg
Keyword(s):  
Pseudomonas Aeruginosa

306: Relationships between Biofilm-Forming Capacities of Pseudomonas Aeruginosa Isolates and Clinical Background / Antimicrobial Resistence in Urinary Tract Infections

2007 ◽  
Vol 177 (4S) ◽  
pp. 102-103
Author(s):  
Shinya Uehara ◽  
Koichi Monden ◽  
Koichiro Wada ◽  
Ayano Ishii ◽  
Reiko Kariyama ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Clinical Background ◽  
Tract Infections

Green striped nails: chromonychia due to Pseudomonas aeruginosa

1968 ◽  
Vol 97 (2) ◽  
pp. 149-153 ◽  
Author(s):  
W. V. Shellow
Keyword(s):  
Pseudomonas Aeruginosa

Effekt von Acetylcystein auf die Pseudomonas aeruginosa-induzierte Mukusexpression

Pneumologie ◽  
2010 ◽  
Vol 64 (01) ◽  
Author(s):  
L Sprenger ◽  
T Goldmann ◽  
E Vollmer ◽  
B Wollenberg ◽  
P Zabel ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa

Effekt von Dexamethason auf die Pseudomonas aeruginosa-induzierte Mukusexpression in humaner Atemwegsmukosa

Pneumologie ◽  
2010 ◽  
Vol 64 (S 03) ◽  
Author(s):  
L Spenger ◽  
T Goldmann ◽  
E Vollmer ◽  
B Wollenberg ◽  
HP Hauber ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa

Pseudomonas aeruginosa acquisition in cystic fibrosis: a role for surfactant protein D and mannose-binding lectin?

2010 ◽  
Vol 222 (S 01) ◽  
Author(s):  
E Tutdibi ◽  
A Schwarz ◽  
D Monz ◽  
G Dockter ◽  
L Gortner
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Surfactant Protein ◽  
Mannose Binding Lectin ◽  
Surfactant Protein D ◽  
Mannose Binding ◽  
Binding Lectin
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