Site-Specific Turn-On Fluorescent Labeling of DNA-Interacting Protein Using Oligodeoxynucleotides That Modify Lysines To Produce 5,6-Dimethoxy 3-Methyleneisoindolin-1-one

2016 ◽  
Vol 11 (8) ◽  
pp. 2216-2221 ◽  
Author(s):  
Chiemi Gatanaga ◽  
Bo Yang ◽  
Yuka Inadomi ◽  
Kazuteru Usui ◽  
Chiyoe Ota ◽  
...  
Keyword(s):  
Fluorescent Labeling ◽  
Interacting Protein ◽  
Site Specific ◽  
Turn On

Site-Specific Fluorescent Labeling of Argonaute for FRET-Based Bio-Assays

2016 ◽  
pp. 291-304 ◽  
Author(s):  
Sarah Willkomm ◽  
Adrian Zander ◽  
Dina Grohmann
Keyword(s):  
Fluorescent Labeling ◽  
Site Specific

scholarly journals Stable, Site-Specific Fluorescent Tagging Constructs Optimized for Burkholderia Species

2010 ◽  
Vol 76 (22) ◽  
pp. 7635-7640 ◽  
Author(s):  
Michael H. Norris ◽  
Yun Kang ◽  
Bruce Wilcox ◽  
Tung T. Hoang
Keyword(s):  
Burkholderia Pseudomallei ◽  
Fluorescent Proteins ◽  
Fluorescent Labeling ◽  
Site Specific ◽  
Burkholderia Thailandensis ◽  
Burkholderia Species ◽  
Fluorescent Tagging

ABSTRACT Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and Burkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized for Burkholderia spp., enabling bacterial tracking during cellular infection.

DNA-binding regulates site-specific ubiquitination of IRF-1

2013 ◽  
Vol 449 (3) ◽  
pp. 707-717 ◽  
Author(s):  
Vivien Landré ◽  
Emmanuelle Pion ◽  
Vikram Narayan ◽  
Dimitris P. Xirodimas ◽  
Kathryn L. Ball
Keyword(s):  
Dna Binding ◽  
E3 Ligase ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Docking Site ◽  
Interacting Protein ◽  
Site Specific ◽  
E3 Ligases ◽  
Intrinsically Disordered ◽  
C Terminus

Understanding the determinants for site-specific ubiquitination by E3 ligase components of the ubiquitin machinery is proving to be a challenge. In the present study we investigate the role of an E3 ligase docking site (Mf2 domain) in an intrinsically disordered domain of IRF-1 [IFN (interferon) regulatory factor-1], a short-lived IFNγ-regulated transcription factor, in ubiquitination of the protein. Ubiquitin modification of full-length IRF-1 by E3 ligases such as CHIP [C-terminus of the Hsc (heat-shock cognate) 70-interacting protein] and MDM2 (murine double minute 2), which dock to the Mf2 domain, was specific for lysine residues found predominantly in loop structures that extend from the DNA-binding domain, whereas no modification was detected in the more conformationally flexible C-terminal half of the protein. The E3 docking site was not available when IRF-1 was in its DNA-bound conformation and cognate DNA-binding sequences strongly suppressed ubiquitination, highlighting a strict relationship between ligase binding and site-specific modification at residues in the DNA-binding domain. Hyperubiquitination of a non-DNA-binding mutant supports a mechanism where an active DNA-bound pool of IRF-1 is protected from polyubiquitination and degradation.

Site-Specific Fluorescent Labeling Approaches for Naringenin, an Essential Flavonone in Plant Nitrogen-Fixation Signaling Pathways

2008 ◽  
Vol 73 (21) ◽  
pp. 8279-8285 ◽  
Author(s):  
Lei Chen ◽  
Feng-Qing Li ◽  
Bi-He Hou ◽  
Guo-Fan Hong ◽  
Zhu-Jun Yao
Keyword(s):  
Nitrogen Fixation ◽  
Signaling Pathways ◽  
Fluorescent Labeling ◽  
Site Specific ◽  
Plant Nitrogen

scholarly journals Fluorescent turn-on probes for wash-free mRNA imaging via covalent site-specific enzymatic labeling

2017 ◽  
Vol 8 (10) ◽  
pp. 7169-7173 ◽  
Author(s):  
Cun Yu Zhou ◽  
Seth C. Alexander ◽  
Neal K. Devaraj
Keyword(s):  
Cellular Regulation ◽  
Site Specific ◽  
Turn On ◽  
The Many ◽  
And Function

Investigating the many roles RNA plays in cellular regulation and function has increased demand for tools to explore RNA tracking and localization within cells.

scholarly journals Site-Specific Fluorescent Labeling of Nascent Proteins on the Translating Ribosome

2011 ◽  
Vol 133 (38) ◽  
pp. 14936-14939 ◽  
Author(s):  
Ishu Saraogi ◽  
Dawei Zhang ◽  
Sandhya Chandrasekaran ◽  
Shu-ou Shan
Keyword(s):  
Fluorescent Labeling ◽  
Site Specific

scholarly journals Site-Specific Fluorescent Labeling and Oriented Immobilization of a Triple Mutant of CYP3A4 via C64

2012 ◽  
Vol 23 (4) ◽  
pp. 826-836 ◽  
Author(s):  
Amélie Ménard ◽  
Yue Huang ◽  
Pierre Karam ◽  
Gonzalo Cosa ◽  
Karine Auclair
Keyword(s):  
Fluorescent Labeling ◽  
Triple Mutant ◽  
Site Specific ◽  
Oriented Immobilization

scholarly journals Site-Specific Fluorescent Labeling of Purified G-Protein-Coupled Receptors Using Genetically-Encoded Unnatural Amino Acids

2010 ◽  
Vol 98 (3) ◽  
pp. 291a-292a
Author(s):  
Shixin Ye ◽  
Manija A. Kazmi ◽  
Terence Duarte ◽  
Saranga Naganathan ◽  
Thomas P. Sakmar ◽  
...  
Keyword(s):  
Amino Acids ◽  
G Protein ◽  
Unnatural Amino Acids ◽  
Fluorescent Labeling ◽  
G Protein Coupled Receptors ◽  
Site Specific ◽  
G Protein Coupled
Sign in / Sign up

Export Citation Format

Share Document