Site-Specific Incorporation of Fluorescent Probes into Protein:  Hexahistidine-Tag-Mediated Fluorescent Labeling with (Ni2+:Nitrilotriacetic Acid)n−Fluorochrome Conjugates

2001 ◽  
Vol 123 (48) ◽  
pp. 12123-12125 ◽  
Author(s):  
Achillefs N. Kapanidis ◽  
Yon W. Ebright ◽  
Richard H. Ebright
Keyword(s):  
Fluorescent Probes ◽  
Nitrilotriacetic Acid ◽  
Fluorescent Labeling ◽  
Site Specific ◽  
Hexahistidine Tag

Site-Specific Fluorescent Labeling of Argonaute for FRET-Based Bio-Assays

2016 ◽  
pp. 291-304 ◽  
Author(s):  
Sarah Willkomm ◽  
Adrian Zander ◽  
Dina Grohmann
Keyword(s):  
Fluorescent Labeling ◽  
Site Specific

scholarly journals The Development of Methods for the Site-Specific Fluorescent Labeling of Spliceosomal Proteins for use in Single-Molecule Studies

2012 ◽  
Vol 102 (3) ◽  
pp. 484a
Author(s):  
Matthew L. Kahlscheuer ◽  
Nils G. Walter
Keyword(s):  
Single Molecule ◽  
Fluorescent Labeling ◽  
Site Specific ◽  
Single Molecule Studies

scholarly journals Stable, Site-Specific Fluorescent Tagging Constructs Optimized for Burkholderia Species

2010 ◽  
Vol 76 (22) ◽  
pp. 7635-7640 ◽  
Author(s):  
Michael H. Norris ◽  
Yun Kang ◽  
Bruce Wilcox ◽  
Tung T. Hoang
Keyword(s):  
Burkholderia Pseudomallei ◽  
Fluorescent Proteins ◽  
Fluorescent Labeling ◽  
Site Specific ◽  
Burkholderia Thailandensis ◽  
Burkholderia Species ◽  
Fluorescent Tagging

ABSTRACT Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and Burkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized for Burkholderia spp., enabling bacterial tracking during cellular infection.

Site-Specific Fluorescent Labeling Approaches for Naringenin, an Essential Flavonone in Plant Nitrogen-Fixation Signaling Pathways

2008 ◽  
Vol 73 (21) ◽  
pp. 8279-8285 ◽  
Author(s):  
Lei Chen ◽  
Feng-Qing Li ◽  
Bi-He Hou ◽  
Guo-Fan Hong ◽  
Zhu-Jun Yao
Keyword(s):  
Nitrogen Fixation ◽  
Signaling Pathways ◽  
Fluorescent Labeling ◽  
Site Specific ◽  
Plant Nitrogen

scholarly journals Site-Specific Fluorescent Labeling of Nascent Proteins on the Translating Ribosome

2011 ◽  
Vol 133 (38) ◽  
pp. 14936-14939 ◽  
Author(s):  
Ishu Saraogi ◽  
Dawei Zhang ◽  
Sandhya Chandrasekaran ◽  
Shu-ou Shan
Keyword(s):  
Fluorescent Labeling ◽  
Site Specific

scholarly journals Multicolor protein FRET with tryptophan, selective coumarin-cysteine labeling, and genetic acridonylalanine encoding

2017 ◽  
Vol 53 (80) ◽  
pp. 11072-11075 ◽  
Author(s):  
John J. Ferrie ◽  
Naoya Ieda ◽  
Conor M. Haney ◽  
Christopher R. Walters ◽  
Itthipol Sungwienwong ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescent Probes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Site Specific ◽  
Cysteine Labeling ◽  
Förster Resonance

A combination of labeling techniques enables site-specific installation of fluorescent probes for measuring distances within proteins by Förster resonance energy transfer (FRET).

Site-Specific Turn-On Fluorescent Labeling of DNA-Interacting Protein Using Oligodeoxynucleotides That Modify Lysines To Produce 5,6-Dimethoxy 3-Methyleneisoindolin-1-one

2016 ◽  
Vol 11 (8) ◽  
pp. 2216-2221 ◽  
Author(s):  
Chiemi Gatanaga ◽  
Bo Yang ◽  
Yuka Inadomi ◽  
Kazuteru Usui ◽  
Chiyoe Ota ◽  
...  
Keyword(s):  
Fluorescent Labeling ◽  
Interacting Protein ◽  
Site Specific ◽  
Turn On

scholarly journals Site-Specific Fluorescent Labeling and Oriented Immobilization of a Triple Mutant of CYP3A4 via C64

2012 ◽  
Vol 23 (4) ◽  
pp. 826-836 ◽  
Author(s):  
Amélie Ménard ◽  
Yue Huang ◽  
Pierre Karam ◽  
Gonzalo Cosa ◽  
Karine Auclair
Keyword(s):  
Fluorescent Labeling ◽  
Triple Mutant ◽  
Site Specific ◽  
Oriented Immobilization

scholarly journals Site-Specific Fluorescent Labeling of Purified G-Protein-Coupled Receptors Using Genetically-Encoded Unnatural Amino Acids

2010 ◽  
Vol 98 (3) ◽  
pp. 291a-292a
Author(s):  
Shixin Ye ◽  
Manija A. Kazmi ◽  
Terence Duarte ◽  
Saranga Naganathan ◽  
Thomas P. Sakmar ◽  
...  
Keyword(s):  
Amino Acids ◽  
G Protein ◽  
Unnatural Amino Acids ◽  
Fluorescent Labeling ◽  
G Protein Coupled Receptors ◽  
Site Specific ◽  
G Protein Coupled
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