Site-Specific Fluorescent Labeling Approaches for Naringenin, an Essential Flavonone in Plant Nitrogen-Fixation Signaling Pathways

2008 ◽  
Vol 73 (21) ◽  
pp. 8279-8285 ◽  
Author(s):  
Lei Chen ◽  
Feng-Qing Li ◽  
Bi-He Hou ◽  
Guo-Fan Hong ◽  
Zhu-Jun Yao
Keyword(s):  
Nitrogen Fixation ◽  
Signaling Pathways ◽  
Fluorescent Labeling ◽  
Site Specific ◽  
Plant Nitrogen

Systemic control of nodule formation by the plant N demand requires autoregulation dependent and independent mechanisms

2021 ◽  
Author(s):  
Marjorie Pervent ◽  
Ilana Lambert ◽  
Marc Tauzin ◽  
Alicia Karouani ◽  
Martha Nigg ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Nodule Formation ◽  
Atmospheric Nitrogen ◽  
Plant Nitrogen ◽  
Systemic Signaling ◽  
Systemic Control ◽  
Whole Plant ◽  
Strong Control ◽  
Mutant Genes ◽  
N Demand

Abstract In legumes interacting with rhizobia the formation of symbiotic organs involved in the acquisition of atmospheric nitrogen is depending of the plant nitrogen (N) demand. We used Medicago truncatula plants cultivated in split-root systems to discriminate between responses to local and systemic N signalings. We evidenced a strong control of nodule formation by systemic N-signaling but obtained no clear evidence of a local control by mineral nitrogen. Systemic signaling of the plant N demand controls numerous transcripts involved in the root transcriptome reprogramming associated to early rhizobia interaction and nodule formation. SUNN has an important role in this control but major systemic N signaling responses remained active in the sunn mutant. Genes involved in the activation of nitrogen fixation are regulated by systemic N signaling in the mutant, explaining why the hypernodulation phenotype is not associated to a higher nitrogen fixation of the whole plant. The control of the transcriptome reprogramming of nodule formation by systemic N signaling requires other pathway(s) that parallel the SUNN/CLE pathway.

Site-Specific Fluorescent Labeling of Argonaute for FRET-Based Bio-Assays

2016 ◽  
pp. 291-304 ◽  
Author(s):  
Sarah Willkomm ◽  
Adrian Zander ◽  
Dina Grohmann
Keyword(s):  
Fluorescent Labeling ◽  
Site Specific

scholarly journals Aphid infestation differently affects the defences of nitrate-fed and nitrogen-fixing Medicago truncatula and alters symbiotic nitrogen fixation

2020 ◽  
Vol 287 (1934) ◽  
pp. 20201493
Author(s):  
Gaurav Pandharikar ◽  
Jean-Luc Gatti ◽  
Jean-Christophe Simon ◽  
Pierre Frendo ◽  
Marylène Poirié
Keyword(s):  
Nitrogen Fixation ◽  
Medicago Truncatula ◽  
Sinorhizobium Meliloti ◽  
Acyrthosiphon Pisum ◽  
Symbiotic Nitrogen Fixation ◽  
Pea Aphid ◽  
Systemic Resistance ◽  
Aphid Infestation ◽  
Plant Nitrogen ◽  
Pathogenesis Related Protein

Legumes can meet their nitrogen requirements through root nodule symbiosis, which could also trigger plant systemic resistance against pests. The pea aphid Acyrthosiphon pisum , a legume pest, can harbour different facultative symbionts (FS) influencing various traits of their hosts. It is therefore worth determining if and how the symbionts of the plant and the aphid modulate their interaction. We used different pea aphid lines without FS or with a single one ( Hamiltonella defensa , Regiella insecticola, Serratia symbiotica ) to infest Medicago truncatula plants inoculated with Sinorhizobium meliloti (symbiotic nitrogen fixation, SNF) or supplemented with nitrate (non-inoculated, NI). The growth of SNF and NI plants was reduced by aphid infestation, while aphid weight (but not survival) was lowered on SNF compared to NI plants. Aphids strongly affected the plant nitrogen fixation depending on their symbiotic status, suggesting indirect relationships between aphid- and plant-associated microbes. Finally, all aphid lines triggered expression of Pathogenesis-Related Protein 1 ( PR1 ) and Proteinase Inhibitor (PI) , respective markers for salicylic and jasmonic pathways, in SNF plants, compared to only PR1 in NI plants. We demonstrate that the plant symbiotic status influences plant–aphid interactions while that of the aphid can modulate the amplitude of the plant's defence response.

scholarly journals Stable, Site-Specific Fluorescent Tagging Constructs Optimized for Burkholderia Species

2010 ◽  
Vol 76 (22) ◽  
pp. 7635-7640 ◽  
Author(s):  
Michael H. Norris ◽  
Yun Kang ◽  
Bruce Wilcox ◽  
Tung T. Hoang
Keyword(s):  
Burkholderia Pseudomallei ◽  
Fluorescent Proteins ◽  
Fluorescent Labeling ◽  
Site Specific ◽  
Burkholderia Thailandensis ◽  
Burkholderia Species ◽  
Fluorescent Tagging

ABSTRACT Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and Burkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized for Burkholderia spp., enabling bacterial tracking during cellular infection.

scholarly journals Site-Specific Fluorescent Labeling of Nascent Proteins on the Translating Ribosome

2011 ◽  
Vol 133 (38) ◽  
pp. 14936-14939 ◽  
Author(s):  
Ishu Saraogi ◽  
Dawei Zhang ◽  
Sandhya Chandrasekaran ◽  
Shu-ou Shan
Keyword(s):  
Fluorescent Labeling ◽  
Site Specific

Site-Specific Turn-On Fluorescent Labeling of DNA-Interacting Protein Using Oligodeoxynucleotides That Modify Lysines To Produce 5,6-Dimethoxy 3-Methyleneisoindolin-1-one

2016 ◽  
Vol 11 (8) ◽  
pp. 2216-2221 ◽  
Author(s):  
Chiemi Gatanaga ◽  
Bo Yang ◽  
Yuka Inadomi ◽  
Kazuteru Usui ◽  
Chiyoe Ota ◽  
...  
Keyword(s):  
Fluorescent Labeling ◽  
Interacting Protein ◽  
Site Specific ◽  
Turn On

scholarly journals Site-Specific Fluorescent Labeling and Oriented Immobilization of a Triple Mutant of CYP3A4 via C64

2012 ◽  
Vol 23 (4) ◽  
pp. 826-836 ◽  
Author(s):  
Amélie Ménard ◽  
Yue Huang ◽  
Pierre Karam ◽  
Gonzalo Cosa ◽  
Karine Auclair
Keyword(s):  
Fluorescent Labeling ◽  
Triple Mutant ◽  
Site Specific ◽  
Oriented Immobilization

Fixation, accumulation, and distribution of nitrogen in a crop of Lupinus angustifolius cv. Unicrop

1977 ◽  
Vol 28 (2) ◽  
pp. 237
Author(s):  
P Farrington ◽  
EAN Greenwood ◽  
ZV Titmanis ◽  
MJ Trinick ◽  
DW Smith
Keyword(s):  
Nitrogen Fixation ◽  
Nitrogen Content ◽  
Acetylene Reduction ◽  
Grain Filling ◽  
Plant Nitrogen ◽  
Balance Sheets ◽  
Reduction Assay ◽  
Beginning Of Flowering ◽  
Proportional Distribution

A lupin crop was sampled each week to measure nitrogen fixation by acetylene reduction assay and for determination of the total nitrogen content on the organs on each axis of the plant. Nitrogen fixation started 5 weeks after sowing, reached its maximum rate per plant at the beginning of flowering on the main axis, and ceased during the period of rapid grain filling, which was 4 weeks before maturity. Plants did not accumulate measurable quantities of nitrogen until 2 weeks after the start of nodular fixation as indicated by acetylene reduction. In the vegetative phase within each order of axes most nitrogen went to the leaves before they senesced. During the first half of the period of rapid grain filling, both the weight and the concentration of nitrogen in the grain increased at the expense of the vegetative components. Balance sheets for nitrogen content and the current proportional distribution of nitrogen are presented for three occasions at weeks 8-9, weeks 15-16 and weeks 18–19. Waterlogging greatly decreased acetylene reduction and plant growth.

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