Quantitative Detection of Cathepsin B Activity in Neutral pH Buffers Using Gold Microelectrode Arrays: Toward Direct Multiplex Analyses of Extracellular Proteases in Human Serum

ACS Sensors ◽  
2021 ◽  
Author(s):  
Yang Song ◽  
Jestin Gage Wright ◽  
Morgan J. Anderson ◽  
Sabari Rajendran ◽  
Zhaoyang Ren ◽  
...  
Keyword(s):  
Human Serum ◽  
Cathepsin B ◽  
Microelectrode Arrays ◽  
Quantitative Detection ◽  
Extracellular Proteases ◽  
Neutral Ph ◽  
Gold Microelectrode

scholarly journals The active-site residue Cys-29 is responsible for the neutral-pH inactivation and the refolding barrier of human cathepsin B

FEBS Letters ◽  
2000 ◽  
Vol 475 (3) ◽  
pp. 157-162 ◽  
Author(s):  
Jianxing Song ◽  
Ping Xu ◽  
Hui Xiang ◽  
Zhengding Su ◽  
Andrew C. Storer ◽  
...  
Keyword(s):  
Active Site ◽  
Cathepsin B ◽  
Active Site Residue ◽  
Neutral Ph ◽  
Ph Inactivation ◽  
Human Cathepsin

scholarly journals Gold Microelectrode Arrays Based Electrode for Determination of Trace Copper in Seawater

2014 ◽  
Vol 26 (9) ◽  
pp. 2741-2744 ◽  
Author(s):  
Shijie Wu ◽  
Dawei Pan ◽  
Zhengyin Yu ◽  
Qi Kang ◽  
Dazhong Shen
Keyword(s):  
Microelectrode Arrays ◽  
Gold Microelectrode

Interference-free SERS tags for ultrasensitive quantitative detection of tyrosinase in human serum based on magnetic bead separation

2020 ◽  
Vol 1138 ◽  
pp. 150-157
Author(s):  
Dechan Lu ◽  
Xueliang Lin ◽  
Cairou Chen ◽  
Yudong Lu ◽  
Shangyuan Feng ◽  
...  
Keyword(s):  
Human Serum ◽  
Magnetic Bead ◽  
Quantitative Detection ◽  
Magnetic Bead Separation ◽  
Sers Tags

Synthesis of novel cationic carbon dots and application to quantitative detection of K+ in human serum samples

2019 ◽  
Vol 43 (46) ◽  
pp. 17937-17940 ◽  
Author(s):  
Zideng Gao ◽  
Shunyi Wang ◽  
Zijun Xu ◽  
Jin Liu ◽  
Yuanfang Huang ◽  
...  
Keyword(s):  
Human Serum ◽  
Carbon Dots ◽  
High Sensitivity ◽  
Quantitative Detection ◽  
Serum Samples ◽  
Human Serum Samples

Novel cationic carbon dots were synthesized in a simple way and applied to detect K+ in human serum samples with ultra-high sensitivity.

Conical recessed gold microelectrode arrays produced during photolithographic methods: Characterisation and causes

2007 ◽  
Vol 9 (5) ◽  
pp. 879-885 ◽  
Author(s):  
F.A. Aguiar ◽  
A.J. Gallant ◽  
M.C. Rosamond ◽  
A. Rhodes ◽  
D. Wood ◽  
...  
Keyword(s):  
Microelectrode Arrays ◽  
Gold Microelectrode

Modified method of erythropoietin (ESF) bioassay in vitro using mouse fetal liver cells. II. measurement of ESF in human serum

Blood ◽  
1978 ◽  
Vol 52 (3) ◽  
pp. 569-577 ◽  
Author(s):  
G de Klerk ◽  
AA Hart ◽  
C Kruiswijk ◽  
R Goudsmit
Keyword(s):  
Human Serum ◽  
Fetal Liver ◽  
Internal Standard ◽  
Immune Serum ◽  
Quantitative Detection ◽  
Modified Method ◽  
The Difference ◽  
Normal Males ◽  
Test Serum

Abstract A modification of the mouse fetal liver cell bioassay for erythropoietin (ESF) that allows quantitative detection of ESF in human serum is described. The modification consists of (1) correction for the effect of serum iron on 59Fe incorporation into heme and (2) the use of an “internal standard,” i.e., a standard ESF preparation dissolved in the assayed test serum. As a result of this modification the statistical method of bioassay analysis had to be changed fundamentally. The mean serum concentration of ESF measured in 20 normal males was 48 microunit/ml, as compared to 29 microunit/ml in 18 females. The difference was significant at p = 0.017. The stimulatory activity of a human serum on 59Fe incorporation into heme could be neutralized by an anti-human ESF immune serum.

scholarly journals Functionalized nanoporous gold as a new biosensor platform for ultra-low quantitative detection of human serum albumin

2019 ◽  
Vol 288 ◽  
pp. 460-468 ◽  
Author(s):  
F. Scaglione ◽  
E. Alladio ◽  
A. Damin ◽  
F. Turci ◽  
C. Baggiani ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Nanoporous Gold ◽  
Quantitative Detection

All-inkjet-printed gold microelectrode arrays for extracellular recording of action potentials

2017 ◽  
Vol 2 (3) ◽  
pp. 035003 ◽  
Author(s):  
Bernd Bachmann ◽  
Nouran Y Adly ◽  
Jan Schnitker ◽  
Alexey Yakushenko ◽  
Philipp Rinklin ◽  
...  
Keyword(s):  
Action Potentials ◽  
Microelectrode Arrays ◽  
Extracellular Recording ◽  
Gold Microelectrode

Desorption of Antigens from Disulphide-linked Immunosorbents: Recovery of Human Serum Albumin at Near Neutral pH

Nature ◽  
1968 ◽  
Vol 218 (5143) ◽  
pp. 773-774 ◽  
Author(s):  
J. W. CHIDLOW ◽  
J. STEPHEN ◽  
H. SMITH
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Neutral Ph
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