A Lambda Red and FLP/FRT-Mediated Site-Specific Recombination System in Komagataeibacter xylinus and Its Application to Enhance the Productivity of Bacterial Cellulose

2020 ◽  
Vol 9 (11) ◽  
pp. 3171-3180
Author(s):  
Ling-Pu Liu ◽  
Xue Yang ◽  
Xiang-Jun Zhao ◽  
Kai-Yue Zhang ◽  
Wen-Chao Li ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Recombination System ◽  
Komagataeibacter Xylinus ◽  
Site Specific Recombination System

scholarly journals Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system

2012 ◽  
Vol 41 (2) ◽  
pp. e37-e37 ◽  
Author(s):  
Madina Karimova ◽  
Josephine Abi-Ghanem ◽  
Nicolas Berger ◽  
Vineeth Surendranath ◽  
Maria Teresa Pisabarro ◽  
...  
Keyword(s):  
Site Specific ◽  
Site Specific Recombination ◽  
Recombination System ◽  
Site Specific Recombination System

scholarly journals The Site-Specific Recombination System of the Escherichia coli Bacteriophage Φ24B

2020 ◽  
Vol 11 ◽  
Author(s):  
Mohammed Radhi Mohaisen ◽  
Alan John McCarthy ◽  
Evelien M. Adriaenssens ◽  
Heather Elizabeth Allison
Keyword(s):  
Escherichia Coli ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Recombination System ◽  
Site Specific Recombination System

scholarly journals Site-Specific Integrative Elements of Rhizobiophage 16-3 Can Integrate into Proline tRNA (CGG) Genes in Different Bacterial Genera

2002 ◽  
Vol 184 (1) ◽  
pp. 177-182 ◽  
Author(s):  
Szabolcs Semsey ◽  
Béla Blaha ◽  
Krisztián Köles ◽  
László Orosz ◽  
Péter P. Papp
Keyword(s):  
Rhizobium Meliloti ◽  
Trna Genes ◽  
Target Sequence ◽  
Integrase Gene ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Tyrosine Recombinases ◽  
Recombination System ◽  
Integrase Protein ◽  
Site Specific Recombination System

ABSTRACT The integrase protein of the Rhizobium meliloti 41 phage 16-3 has been classified as a member of the Int family of tyrosine recombinases. The site-specific recombination system of the phage belongs to the group in which the target site of integration (attB) is within a tRNA gene. Since tRNA genes are conserved, we expected that the target sequence of the site-specific recombination system of the 16-3 phage could occur in other species and integration could take place if the required putative host factors were also provided by the targeted cells. Here we report that a plasmid (pSEM167) carrying the attP element and the integrase gene (int) of the phage can integrate into the chromosomes of R. meliloti 1021 and eight other species. In all cases integration occurred at so-far-unidentified, putative proline tRNA (CGG) genes, indicating the possibility of their common origin. Multiple alignment of the sequences suggested that the location of the att core was different from that expected previously. The minimal attB was identified as a 23-bp sequence corresponding to the anticodon arm of the tRNA.

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