Engineering a Synthetic Pathway for Tyrosol Synthesis in Escherichia coli

2022 ◽  
Author(s):  
Yumeng Lai ◽  
Haofeng Chen ◽  
Lingrui Liu ◽  
Bixia Fu ◽  
Peiling Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Synthetic Pathway

scholarly journals Engineered Synthetic Pathway for Isopropanol Production in Escherichia coli

2007 ◽  
Vol 73 (24) ◽  
pp. 7814-7818 ◽  
Author(s):  
T. Hanai ◽  
S. Atsumi ◽  
J. C. Liao
Keyword(s):  
Escherichia Coli ◽  
Secondary Alcohol ◽  
Clostridium Beijerinckii ◽  
Synthetic Pathway ◽  
E Coli ◽  
Coa Transferase ◽  
Secondary Alcohol Dehydrogenase ◽  
Acetoacetate Decarboxylase ◽  
Shake Flasks ◽  
K 12

ABSTRACT A synthetic pathway was engineered in Escherichia coli to produce isopropanol by expressing various combinations of genes from Clostridium acetobutylicum ATCC 824, E. coli K-12 MG1655, Clostridium beijerinckii NRRL B593, and Thermoanaerobacter brockii HTD4. The strain with the combination of C. acetobutylicum thl (acetyl-coenzyme A [CoA] acetyltransferase), E. coli atoAD (acetoacetyl-CoA transferase), C. acetobutylicum adc (acetoacetate decarboxylase), and C. beijerinckii adh (secondary alcohol dehydrogenase) achieved the highest titer. This strain produced 81.6 mM isopropanol in shake flasks with a yield of 43.5% (mol/mol) in the production phase. To our knowledge, this work is the first to produce isopropanol in E. coli, and the titer exceeded that from the native producers.

scholarly journals Production of Glucaric Acid from a Synthetic Pathway in Recombinant Escherichia coli

2008 ◽  
Vol 75 (3) ◽  
pp. 589-595 ◽  
Author(s):  
Tae Seok Moon ◽  
Sang-Hwal Yoon ◽  
Amanda M. Lanza ◽  
Joseph D. Roy-Mayhew ◽  
Kristala L. Jones Prather
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Syringae ◽  
Glucuronic Acid ◽  
Value Added ◽  
Culture Broth ◽  
Glucaric Acid ◽  
Synthetic Pathway ◽  
Genes Encoding ◽  
Microbial System ◽  
Rate Limiting

ABSTRACT A synthetic pathway has been constructed for the production of glucuronic and glucaric acids from glucose in Escherichia coli. Coexpression of the genes encoding myo-inositol-1-phosphate synthase (Ino1) from Saccharomyces cerevisiae and myo-inositol oxygenase (MIOX) from mice led to production of glucuronic acid through the intermediate myo-inositol. Glucuronic acid concentrations up to 0.3 g/liter were measured in the culture broth. The activity of MIOX was rate limiting, resulting in the accumulation of both myo-inositol and glucuronic acid as final products, in approximately equal concentrations. Inclusion of a third enzyme, uronate dehydrogenase (Udh) from Pseudomonas syringae, facilitated the conversion of glucuronic acid to glucaric acid. The activity of this recombinant enzyme was more than 2 orders of magnitude higher than that of Ino1 and MIOX and increased overall flux through the pathway such that glucaric acid concentrations in excess of 1 g/liter were observed. This represents a novel microbial system for the biological production of glucaric acid, a “top value-added chemical” from biomass.

Dual synthetic pathway for 3-hydroxypropionic acid production in engineered Escherichia coli

2015 ◽  
Vol 120 (2) ◽  
pp. 199-204 ◽  
Author(s):  
Hiroshi Honjo ◽  
Keigo Tsuruno ◽  
Tsuneyuki Tatsuke ◽  
Masaki Sato ◽  
Taizo Hanai
Keyword(s):  
Escherichia Coli ◽  
Acid Production ◽  
Synthetic Pathway ◽  
Engineered Escherichia Coli ◽  
Hydroxypropionic Acid

scholarly journals n-Butylamine production from glucose using a transaminase-mediated synthetic pathway in Escherichia coli

2020 ◽  
Vol 129 (1) ◽  
pp. 99-103
Author(s):  
Takuya Matsumoto ◽  
Yuki Mori ◽  
Tsutomu Tanaka ◽  
Akihiko Kondo
Keyword(s):  
Escherichia Coli ◽  
Synthetic Pathway

scholarly journals Combinatorial modulation of initial codons for improved zeaxanthin synthetic pathway efficiency in Escherichia coli

2019 ◽  
Vol 8 (12) ◽  
Author(s):  
Zaiqiang Wu ◽  
Dongdong Zhao ◽  
Siwei Li ◽  
Junsong Wang ◽  
Changhao Bi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Synthetic Pathway

Construction of a novel phenol synthetic pathway in Escherichia coli through 4-hydroxybenzoate decarboxylation

2015 ◽  
Vol 99 (12) ◽  
pp. 5163-5173 ◽  
Author(s):  
Liangtian Miao ◽  
Qingyan Li ◽  
Aipo Diao ◽  
Xueli Zhang ◽  
Yanhe Ma
Keyword(s):  
Escherichia Coli ◽  
Synthetic Pathway

scholarly journals Optimization of ethylene glycol production from (d)-xylose via a synthetic pathway implemented in Escherichia coli

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Ceren Alkim ◽  
Yvan Cam ◽  
Debora Trichez ◽  
Clément Auriol ◽  
Lucie Spina ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ethylene Glycol ◽  
Synthetic Pathway

scholarly journals Engineering a synthetic pathway for maleate in Escherichia coli

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Shuhei Noda ◽  
Tomokazu Shirai ◽  
Yutaro Mori ◽  
Sachiko Oyama ◽  
Akihiko Kondo
Keyword(s):  
Escherichia Coli ◽  
Synthetic Pathway
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