Studies on the Hill reaction of membrane fragments of blue-green algae IV. Garotenoid photobleaching induced by photosystem II action

1984 ◽  
Vol 39 (5) ◽  
pp. 374-377 ◽  
Author(s):  
J. J. S. van Rensen

The reactivation of the Hill reaction in CO2-depleted broken chloroplasts by various concentrations of bicarbonate was measured in the absence and in the presence of photosystem II herbicides. It appears that these herbicides decrease the apparent affinity of the thylakoid membrane for bicarbonate. Different characteristics of bicarbonate binding were observed in chloroplasts of triazine-resistant Amaranthus hybridus compared to the triazine-sensitive biotype. It is concluded that photosystem II herbicides, bicarbonate and formate interact with each other in their binding to the Qв-protein and their interference with photosynthetic electron transport.


1973 ◽  
Vol 12 (2) ◽  
pp. 391-401
Author(s):  
R. MACHE ◽  
S. LOISEAUX

The growth rate of the shade plant Marchantia was at its maximum for a low illumination, 2-3 x 103 lx, and was inhibited by an excess of light. Photosynthesis by intact thalli and by isolated chloroplasts of Marchantia was saturated by a light intensity of 2-3 x 103 lx. These isolated chloroplasts were able to carry on satisfactory rates of photosynthesis, up to 35 µM CO2/h/mg chlorophyll. The Hill reaction and photosystem II were also saturated by the same light intensities, demonstrating that the factor limiting the light saturation of photosynthesis is located in the chloroplast. The structure of chloroplasts was strongly modified by an excess of light, small grana and fret membranes being replaced by continuous grana.


1970 ◽  
Vol 25 (10) ◽  
pp. 1157-1159 ◽  
Author(s):  
A. Trebst ◽  
E. Harth ◽  
W. Draber

A halogenated benzoquinone has been found to inhibit the photosynthetic electron transport system in isolated chloroplasts. 2·10-6ᴍ of dibromo-thymoquinone inhibit the Hill- reaction with NADP, methylviologen or anthraquinone to 100%, but do not effect the photoreduction of NADP at the expense of an artificial electron donor. The Hill - reaction with ferricyanide is inhibited even at the high concentration of 2·10-5ᴍ of dibromo-thymoquinone to only 60%. The remaining reduction in the presence of the inhibitor reflects the rate of ferricyanide reduction by photosystem II. It is concluded that the inhibition of electron transport by the quinone occurs between photosystem I and II and close to or at the functional site of plastoquinone.


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