Positive Detection of GPCR Antagonists Using a System for Inverted Expression of a Fluorescent Reporter Gene

2017 ◽  
Vol 6 (8) ◽  
pp. 1554-1562 ◽  
Author(s):  
Nobuo Fukuda ◽  
Misato Kaishima ◽  
Jun Ishii ◽  
Shinya Honda
Keyword(s):  
Reporter Gene ◽  
Fluorescent Reporter

scholarly journals Construction of a Fluorescent Reporter Gene for the Analysis of OGDH2 Protein Stability in Hypoxia

2015 ◽  
Vol 120 (3) ◽  
pp. e146
Author(s):  
W. O'NEILL ◽  
R. SUN ◽  
I. PAPANDREOU ◽  
J. KALMAR ◽  
N. DENKO
Keyword(s):  
Protein Stability ◽  
Reporter Gene ◽  
Fluorescent Reporter

scholarly journals A fluorescent reporter gene as a marker for ventricular specification in ES-derived cardiac cells

FEBS Letters ◽  
2000 ◽  
Vol 478 (1-2) ◽  
pp. 151-158 ◽  
Author(s):  
Nathalie Meyer ◽  
Marisa Jaconi ◽  
Angela Landopoulou ◽  
Philippe Fort ◽  
Michel Pucéat
Keyword(s):  
Reporter Gene ◽  
Cardiac Cells ◽  
Fluorescent Reporter

scholarly journals Using a Single Fluorescent Reporter Gene to Infer Half-Life of Extrinsic Noise and Other Parameters of Gene Expression

2010 ◽  
Vol 98 (12) ◽  
pp. 2759-2769 ◽  
Author(s):  
Michał Komorowski ◽  
Bärbel Finkenstädt ◽  
David Rand
Keyword(s):  
Gene Expression ◽  
Reporter Gene ◽  
Half Life ◽  
Extrinsic Noise ◽  
Fluorescent Reporter

scholarly journals Generation of Transgenic Medaka Oryzias curvinotus (Nichols & Pope, 1927) Carrying a Cyan Fluorescent Protein Gene Driven by Alpha Actin Promoter

2021 ◽  
Vol 34 (1) ◽  
Author(s):  
VY NGUYEN HOANG THUY ◽  
TRUNG MAI NGUYEN THANH ◽  
BINH NGUYEN QUOC ◽  
HOA NGUYEN THI KIEU ◽  
DU NGUYEN VAN ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Fluorescent Protein ◽  
Cyan Fluorescent Protein ◽  
Cell Stage ◽  
Fluorescent Reporter ◽  
Myosin Light Chain 2 ◽  
Oryzias Curvinotus ◽  
The Stability ◽  
Green Fluorescent ◽  
Alpha Actin

The study aimed to produce fluorescent protein transgenic medaka Oryzias curvinotus (Nichols & Pope, 1927) as a novel strain of ornamental fish. These fish were produced by transferring a plasmid consisting of a fluorescent reporter gene and a strong promoter into one-cell stage embryos. For this purpose, myosin light chain 2, but not other promoters, was mainly used. The study also evaluated the stability of the transgenic medaka germline acquiring vivid fluorescent phenotypes via the transgenesis of the cyan fluorescent protein (CFP) gene under the control of O. curvinotus skeletal alpha-actin (OCacta) promoter. The pOCacta-CFP plasmid, containing a OCacta promoter and CFP reporter gene, was transferred into the one-cell stage of O. curvinotus embryos by a microinjection technique. As a result, 36 of 1386 microinjected O. curvinotus embryos exhibited CFP signals in their trunks. The expressed CFP signals in O. curvinotus embryos and adults were detected under a microscope using a green fluorescent protein (GFP) filter (450–490 nm wavelength), and blue LED light (400–450 nm wavelength). Five O. curvinotus founders showing clear CFP signals were selected and crossed with non-transgenic counterparts to produce subsequent generations. Among strains, the frequency of germline transmission from founder to F1 was highly variable. Only two of the five founders successfully pass the transgene to the F1 generation. At present, the progeny of subsequent generations is being produced and tested for the expression of CFP signals, and therefore, stable lines are ongoing.

scholarly journals WellInverter: a web application for the analysis of fluorescent reporter gene data

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Yannick Martin ◽  
Michel Page ◽  
Christophe Blanchet ◽  
Hidde de Jong
Keyword(s):  
Reporter Gene ◽  
Web Application ◽  
Fluorescent Reporter ◽  
Gene Data
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