Detection of Oligonucleotide Hybridization on a Single Microparticle by Time-Resolved Fluorometry:  Hybridization Assays on Polymer Particles Obtained by Direct Solid Phase Assembly of the Oligonucleotide Probes

1997 ◽  
Vol 8 (3) ◽  
pp. 378-384 ◽  
Author(s):  
Harri Hakala ◽  
Pia Heinonen ◽  
Antti Iitiä ◽  
Harri Lönnberg
Keyword(s):  
Solid Phase ◽  
Oligonucleotide Probes ◽  
Polymer Particles ◽  
Time Resolved ◽  
Oligonucleotide Hybridization ◽  
Direct Solid ◽  
Hybridization Assays

Direct solid-phase time-resolved immunofluorometric assay of cortisol in serum.

1985 ◽  
Vol 31 (10) ◽  
pp. 1731-1734 ◽  
Author(s):  
J U Eskola ◽  
V Näntö ◽  
L Meurling ◽  
T N Lövgren
Keyword(s):  
Polyclonal Antibody ◽  
Room Temperature ◽  
Solid Phase ◽  
Practical Importance ◽  
Serum Cortisol ◽  
Time Resolved Fluorescence ◽  
Trichloracetic Acid ◽  
Time Resolved ◽  
Sample Separation ◽  
Direct Solid

Abstract A dissociation-enhanced lanthanide fluoroimmunoassay of serum cortisol based on time-resolved fluorescence is described. The assay is a direct assay, where cortisol immobilized on the wall of a microtiter-strip well competes with cortisol in the sample for the europium-labeled polyclonal antibody. The amount of bound europium-labeled antibody is inversely proportional to the amount of cortisol in the sample. Separation is accomplished by washing the strip well. The assay is carried out in 2 h, at room temperature; it is easy to perform and gives accurate and reliable results. A chaotropic agent, trichloracetic acid, was very effective in releasing cortisol from binding proteins. This finding will have practical importance in the immunoassay field.

Direct solid-phase time-resolved fluoroimmunoassay of 17 alpha-hydroxyprogesterone in serum and dried blood spots on filter paper

1990 ◽  
Vol 36 (9) ◽  
pp. 1667-1672 ◽  
Author(s):  
R R Gonzalez ◽  
O Mäentausta ◽  
J Solyom ◽  
R Vihko
Keyword(s):  
Filter Paper ◽  
Specific Activity ◽  
Solid Phase ◽  
High Specific Activity ◽  
Dried Blood Spots ◽  
Serum Samples ◽  
Time Resolved ◽  
Blood Spots ◽  
Phase Time ◽  
Direct Solid

Abstract We describe a direct, solid-phase time-resolved fluoroimmunoassay (TRFIA) for measuring 17 alpha-hydroxyprogesterone (17OHP) in serum and blood spots on filter paper. We used 17OHP-3-carboxymethyloxime (17OHP3CMO) coupled to polylysine as the label, which enabled incorporation of up to 34 atoms of europium per molecule of 17OHP, for a very high specific activity. The assay is based on competition between labeled 17OHP3CMO and 17OHP in blood specimens for polyclonal rabbit anti-17OHP antibodies. The antibody-label complex is separated by binding to anti-rabbit antibodies coated onto microtiter strips. The assay buffer contains danazol to displace 17OHP from steroid-binding proteins in serum. For serum samples, the assay is accomplished in 1 h of incubation at room temperature. The blood spot assay with filter paper discs involves incubation overnight at 4 degrees C. Results for both types of specimens from the same subjects correlated well. The lowest measurable concentrations of 17OHP (nmol/L) were 0.10 (3 SD) and 0.75 (3 SD) for serum and dried blood on filter paper, respectively. Intra- and interassay CVs were about 5-15% for both types of samples.

Non-porous sphérical polymér particlés for luminéscént solid phase detection reactors

1996 ◽  
Vol 320 (1) ◽  
pp. 87-97 ◽  
Author(s):  
Einar Pontén ◽  
Börje Glad ◽  
Malin Stigbrand ◽  
Anna Sjögren ◽  
Knut Irgum
Keyword(s):  
Solid Phase ◽  
Phase Detection ◽  
Polymer Particles

Direct solid-phase synthesis of octreotide conjugates

1999 ◽  
Vol 7 (9) ◽  
pp. 1797-1803 ◽  
Author(s):  
Hsing-Pang Hsieh ◽  
Ying-Ta Wu ◽  
Shui-Tein Chen ◽  
Kung-Tsung Wang
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Phase Synthesis ◽  
Direct Solid

Solute Segregation and Dynamics of Solid-Phase Crystallization In in and Sb-Implanted Silicon

1981 ◽  
Vol 4 ◽  
Author(s):  
J. Narayan ◽  
G. L. Olson ◽  
O. W. Holland
Keyword(s):  
Laser Power ◽  
Solid Phase ◽  
Solute Segregation ◽  
Dislocation Loops ◽  
Solid Phase Crystallization ◽  
Time Resolved ◽  
Plan View ◽  
Ion Channeling ◽  
Retrograde Solubility ◽  
Transmission Electron

ABSTRACTTime-resolved-reflectivity measurements have been combined with transmission electron microscopy (cross-section and plan-view), Rutherford backscattering and ion channeling techniques to study the details of laser induced solid phase epitaxial growth in In+ and Sb+ implanted silicon in the temperature range from 725 to 1500 °K. The details of microstructures including the formation of polycrystals, precipitates, and dislocations have been correlated with the dynamics of crystallization. There were limits to the dopant concentrations which could be incorporated into substitutional lattice sites; these concentrations exceeded retrograde solubility limits by factors up to 70 in the case of the Si-In system. The coarsening of dislocation loops and the formation of a/2<110>, 90° dislocations in the underlying dislocation-loop bands are described as a function of laser power.

Direct solid-phase enzymoimmunoassay of testosterone in saliva.

1989 ◽  
Vol 35 (10) ◽  
pp. 2044-2047 ◽  
Author(s):  
K Howard ◽  
M Kane ◽  
A Madden ◽  
J P Gosling ◽  
P F Fottrell
Keyword(s):  
Detection Limit ◽  
Analytical Procedure ◽  
Solid Phase ◽  
Medical Applications ◽  
Microtiter Plates ◽  
Large Numbers ◽  
Coefficients Of Variation ◽  
Serial Sampling ◽  
Direct Solid

Abstract This competitive, solid-phase enzymoimmunoassay for testosterone in saliva is carried out on microtiter plates and involves no chromatographic or extraction steps. With an overnight incubation the detection limit of the assay is 230 fg per well (16.1 pmol/L). There was a good correlation (correlation coefficient 0.95) between testosterone concentrations measured with and without prior extraction of the saliva samples. Repeated assay of three control saliva samples containing a range of testosterone concentrations (200-1000 pmol/L) gave within- and between-assay coefficients of variation of 5.5-13.2%. The analytical procedure is simple and closely resembles already published procedures for the determination of progesterone and estrone (with extraction) in saliva. One person can assay 200 samples in 24 h and the assay is suitable for reproductive and sports medical applications, particularly for projects involving serial sampling and yielding large numbers of samples.

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