Synthesis and Biological Activity of Polyethylene Glycol−Mouse Nerve Growth Factor Conjugate

1999 ◽  
Vol 10 (6) ◽  
pp. 932-937 ◽  
Author(s):  
Nadya Belcheva ◽  
Kim Woodrow-Mumford ◽  
Melissa J. Mahoney ◽  
W. Mark Saltzman
Keyword(s):  
Biological Activity ◽  
Polyethylene Glycol ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth

TF-1 Cell Proliferation Assay Method for Estimating Bioactivity of Nerve Growth Factor

2013 ◽  
Vol 2 (2) ◽  
pp. 32
Author(s):  
Lingyan Ma ◽  
Yufei Zou
Keyword(s):  
Biological Activity ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Mtt Assay ◽  
Cholinergic Neurons ◽  
Assay Method ◽  
Test Substance ◽  
Nerve Growth ◽  
Standard Curve ◽  
Plate Reader

<p>Nerve growth factor (NGF) is a neurotrophic factor responsible for the differentiation and survival of sympathetic and sensory neurons as well as selective populations of cholinergic neurons. The MTT assay is to add the chromogenic substance MTT after the NGF has stimulated the TF1 cells, to conduct a colorimetric analysis on the micro plate reader, then to compare the results with a standard curve to arrive at the biological activity of the test substance. To use the MTT assay to measure the biological activity of the NGF, it is easy to handle, with good and reliable results, repeatable and with no impact of the human factors.</p>

Recombinant human β-nerve growth factor (NGF): Biological activity and properties in an enzyme immunoassay

1990 ◽  
Vol 27 (4) ◽  
pp. 665-677 ◽  
Author(s):  
S. Söderström ◽  
F. Hallböök ◽  
C. F. Ibáñez ◽  
H. Persson ◽  
T. Ebendal
Keyword(s):  
Biological Activity ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Enzyme Immunoassay ◽  
Nerve Growth

Single-Step Purification and Biological Activity of Human Nerve Growth Factor Produced from Insect Cells

1991 ◽  
Vol 56 (3) ◽  
pp. 1012-1018 ◽  
Author(s):  
Stephen Buxser ◽  
Steven Vroegop ◽  
Douglas Decker ◽  
Jessica Hinzmann ◽  
Roger Poorman ◽  
...  
Keyword(s):  
Biological Activity ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Insect Cells ◽  
Single Step ◽  
Nerve Growth ◽  
Single Step Purification ◽  
Human Nerve

scholarly journals Accumulation of Nerve Growth Factor in Cerebrospinal Fluid and Biological Activity Following Neurosurgery

1995 ◽  
Vol 35 (7) ◽  
pp. 431-437 ◽  
Author(s):  
Masachika SAGOH ◽  
Kazunari YOSHIDA ◽  
Hirooki WAKAMOTO ◽  
Hiroyuki KAMIGUCHI ◽  
Mitsuhiro OTANI ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Biological Activity ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth

7S nerve growth factor has different biological activity from 2.5S nerve growth factor in vitro

1993 ◽  
Vol 609 (1-2) ◽  
pp. 338-340 ◽  
Author(s):  
Ningsheng Shao ◽  
Huixin Wang ◽  
Tingchong Zhou ◽  
Chuan Liu
Keyword(s):  
Biological Activity ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth
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