Inhibition of biological activity of mouse beta-nerve growth factor by monoclonal antibody

Science ◽  
1980 ◽  
Vol 210 (4472) ◽  
pp. 910-912 ◽  
Author(s):  
S. Warren ◽  
M Fanger ◽  
K. Neet
Keyword(s):  
Monoclonal Antibody ◽  
Biological Activity ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth

TF-1 Cell Proliferation Assay Method for Estimating Bioactivity of Nerve Growth Factor

2013 ◽  
Vol 2 (2) ◽  
pp. 32
Author(s):  
Lingyan Ma ◽  
Yufei Zou
Keyword(s):  
Biological Activity ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Mtt Assay ◽  
Cholinergic Neurons ◽  
Assay Method ◽  
Test Substance ◽  
Nerve Growth ◽  
Standard Curve ◽  
Plate Reader

<p>Nerve growth factor (NGF) is a neurotrophic factor responsible for the differentiation and survival of sympathetic and sensory neurons as well as selective populations of cholinergic neurons. The MTT assay is to add the chromogenic substance MTT after the NGF has stimulated the TF1 cells, to conduct a colorimetric analysis on the micro plate reader, then to compare the results with a standard curve to arrive at the biological activity of the test substance. To use the MTT assay to measure the biological activity of the NGF, it is easy to handle, with good and reliable results, repeatable and with no impact of the human factors.</p>

Recombinant human β-nerve growth factor (NGF): Biological activity and properties in an enzyme immunoassay

1990 ◽  
Vol 27 (4) ◽  
pp. 665-677 ◽  
Author(s):  
S. Söderström ◽  
F. Hallböök ◽  
C. F. Ibáñez ◽  
H. Persson ◽  
T. Ebendal
Keyword(s):  
Biological Activity ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Enzyme Immunoassay ◽  
Nerve Growth
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