Site-Directed Mutagenesis Reveals Critical Importance of the Catalytic Site in the Binding of .alpha.-Amylase by Wheat Proteinaceous Inhibitor

Biochemistry ◽  
1994 ◽  
Vol 33 (25) ◽  
pp. 7925-7930 ◽  
Author(s):  
Kenji Takase
Keyword(s):  
Catalytic Site ◽  
Site Directed Mutagenesis ◽  
Alpha Amylase ◽  
Directed Mutagenesis ◽  
Critical Importance ◽  
Proteinaceous Inhibitor

scholarly journals Thimet oligopeptidase: site-directed mutagenesis disproves previous assumptions about the nature of the catalytic site

FEBS Letters ◽  
1998 ◽  
Vol 435 (1) ◽  
pp. 16-20 ◽  
Author(s):  
Jinq-May Chen ◽  
Richard A.E. Stevens ◽  
Paul W. Wray ◽  
Neil D. Rawlings ◽  
Alan J. Barrett
Keyword(s):  
Catalytic Site ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Thimet Oligopeptidase

Alteration of bond-cleavage pattern in the hydrolysis catalyzed by Saccharomycopsis .alpha.-amylase altered by site-directed mutagenesis

Biochemistry ◽  
1992 ◽  
Vol 31 (22) ◽  
pp. 5232-5236 ◽  
Author(s):  
Ikuo Matsui ◽  
Kazuhiko Ishikawa ◽  
Sachio Miyairi ◽  
Sakuzo Fukui ◽  
Koichi Honda
Keyword(s):  
Bond Cleavage ◽  
Site Directed Mutagenesis ◽  
Alpha Amylase ◽  
Directed Mutagenesis ◽  
Cleavage Pattern

Exploration of the catalytic site of endopeptidase 24.11 by site-directed mutagenesis Histidine residues 583 and 587 are essential for catalysis

FEBS Letters ◽  
1988 ◽  
Vol 231 (1) ◽  
pp. 54-58 ◽  
Author(s):  
Alain Devault ◽  
Valérie Sales ◽  
Christiane Nault ◽  
Ann Beaumont ◽  
Bernard Roques ◽  
...  
Keyword(s):  
Catalytic Site ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Histidine Residues ◽  
Endopeptidase 24.11

A conserved cysteine residue in yeast uroporphyrinogen decarboxylase is not essential for enzymatic activity

1997 ◽  
Vol 43 (8) ◽  
pp. 792-795 ◽  
Author(s):  
Celestino Di Flumeri ◽  
Nicholas H. Acheson ◽  
Teresa Keng
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzymatic Activity ◽  
Mutant Strain ◽  
Cysteine Residue ◽  
Catalytic Site ◽  
Heme Biosynthesis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Decarboxylase Activity ◽  
Uroporphyrinogen Decarboxylase

Uroporphyrinogen decarboxylase catalyzes the fifth step of heme biosynthesis in Saccharomyces cerevisiae. Studies utilizing sulfhydryl-specific reagents suggest that the enzyme requires a cysteine residue within the catalytic site This hypothesis was tested directly by site-directed mutagenesis of highly conserved cysteine-52 to serine or alanine. Plasmids containing these mutations were able to complement a hem6 mutant strain. In addition, properties associated with decreased uroporphyrinogen decarboxylase activity were not detected in the mutant strain transformed with these mutant plasmids. These results suggest that the conserved cysteine-52 by itself is not essential for enzymatic activity.Key words: heme biosynthesis, uroporphyrinogen decarboxylase, site-directed mutagenesis.

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