N-Glycosylation and in vitro enzymic activity of human recombinant tissue plasminogen activator expressed in Chinese hamster ovary cells and a murine cell line

Biochemistry ◽  
1989 ◽  
Vol 28 (19) ◽  
pp. 7670-7679 ◽  
Author(s):  
Raj B. Parekh ◽  
Raymond A. Dwek ◽  
Pauline M. Rudd ◽  
Jerry R. Thomas ◽  
Thomas W. Rademacher ◽  
...  
Keyword(s):  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Recombinant Tissue Plasminogen Activator ◽  
Enzymic Activity ◽  
Ovary Cells ◽  
Murine Cell Line ◽  
Tissue Plasminogen

scholarly journals Effect of Temperature Downshift on the Transcriptomic Responses of Chinese Hamster Ovary Cells Using Recombinant Human Tissue Plasminogen Activator Production Culture

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0151529 ◽  
Author(s):  
Andrea Bedoya-López ◽  
Karel Estrada ◽  
Alejandro Sanchez-Flores ◽  
Octavio T. Ramírez ◽  
Claudia Altamirano ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Chinese Hamster Ovary ◽  
Human Tissue ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Effect Of Temperature ◽  
Ovary Cells ◽  
Tissue Plasminogen ◽  
Temperature Downshift ◽  
Transcriptomic Responses

Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro III: Results with 27 chemicals

1989 ◽  
Vol 13 (2) ◽  
pp. 133-193 ◽  
Author(s):  
D. K. Gulati ◽  
K. Witt ◽  
B. Anderson ◽  
E. Zeiger ◽  
M. D. Shelby
Keyword(s):  
Chromosome Aberration ◽  
Sister Chromatid ◽  
Chinese Hamster Ovary ◽  
Sister Chromatid Exchange ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

Chromosome-damaging activity of a ruthenium radio-sensitizer, RuCl2(DMSO)2(4-Nitroimidazole)2, in Chinese hamster ovary cells in vitro

1986 ◽  
Vol 59 ◽  
pp. 247-254 ◽  
Author(s):  
P.K.L. Chan ◽  
K.A. Skov ◽  
B.R. James ◽  
N.P. Farrell
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

Persistence of sister chromatid exchange by 9-β-D-arabinofuranosyladenine in Chinese hamster ovary cells cultivated in vitro

Mutagenesis ◽  
1993 ◽  
Vol 8 (5) ◽  
pp. 445-448 ◽  
Author(s):  
Paolo Perticone ◽  
Marco Linguardo ◽  
Renata Cozzi ◽  
Rosa Maria Corbo ◽  
Stefania Polani
Keyword(s):  
Sister Chromatid ◽  
Chinese Hamster Ovary ◽  
Sister Chromatid Exchange ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

Reduction of endogenous GRP78 levels improves secretion of a heterologous protein in CHO cells

1988 ◽  
Vol 8 (10) ◽  
pp. 4063-4070
Author(s):  
A J Dorner ◽  
M G Krane ◽  
R J Kaufman
Keyword(s):  
Endoplasmic Reticulum ◽  
Chinese Hamster Ovary ◽  
Heterologous Protein ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Glycosylation Site ◽  
Ovary Cells ◽  
Tissue Plasminogen ◽  
Stable Association

GRP78 is localized in the endoplasmic reticulum and associates with improperly folded or underglycosylated proteins. The role of GRP78 in secretion was studied in Chinese hamster ovary cells expressing a tissue plasminogen activator (tPA) variant which lacks potential N-linked glycosylation site sequences because of mutagenesis. The expression of variant tPA resulted in elevated levels of GRP78 and its stable association with tPA. The introduction of antisense GRP78 genes resulted in a two- to threefold reduction in GRP78 levels compared with those of the original cells. Cells with reduced levels of GRP78 secreted two- to threefold-higher levels of tPA activity. tPA expressed in these cells displayed reduced association with GRP78, and a greater proportion was processed to the mature form and secreted. These results demonstrate that reduction of GRP78 level can improve the secretion of an associated protein.

In vitro biosynthesis of diphthamide, studied with mutant Chinese hamster ovary cells resistant to diphtheria toxin

1984 ◽  
Vol 4 (4) ◽  
pp. 642-650
Author(s):  
T J Moehring ◽  
D E Danley ◽  
J M Moehring
Keyword(s):  
Chinese Hamster Ovary ◽  
Diphtheria Toxin ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Elongation Factor ◽  
Synthetase Activity ◽  
Post Translational Modification ◽  
Ovary Cells ◽  
Elongation Factor 2

Diphthamide, a unique amino acid, is a post-translational derivative of histidine that exists in protein synthesis elongation factor 2 at the site of diphtheria toxin-catalyzed ADP-ribosylation of elongation factor 2. We investigated steps in the biosynthesis of diphthamide with mutants of Chinese hamster ovary cells that were altered in different steps of this complex post-translational modification. Biochemical evidence indicates that this modification requires a minimum of three steps, two of which we accomplished in vitro. We identified a methyltransferase activity that transfers methyl groups from S-adenosyl methionine to an unmethylated form of diphthine (the deamidated form of diphthamide), and we tentatively identified an ATP-dependent synthetase activity involved in the biosynthesis of diphthamide from diphthine. Our results are in accord with the proposed structure of diphthamide (B. G. VanNess, et al., J. Biol. Chem. 255:10710-10716, 1980).

scholarly journals Novel Promoters Derived from Chinese Hamster Ovary Cells via In Silico and In Vitro Analysis

2019 ◽  
Vol 14 (11) ◽  
pp. 1900125 ◽  
Author(s):  
Ly N. Nguyen ◽  
Martina Baumann ◽  
Heena Dhiman ◽  
Nicolas Marx ◽  
Valerie Schmieder ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
In Silico ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Vitro Analysis ◽  
In Vitro Analysis
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