Studies on the 5' termini of Novikoff ascites hepatoma ribosomal precursor RNA

Biochemistry ◽  
1977 ◽  
Vol 16 (14) ◽  
pp. 3215-3219 ◽  
Author(s):  
Ross N. Nazar
Keyword(s):  
Ascites Hepatoma ◽  
Precursor Rna ◽  
Ribosomal Precursor

scholarly journals Ribosomal ribonucleic acid and ribosomal precursor ribonucleic acid in Anacystis nidulans

1972 ◽  
Vol 129 (1) ◽  
pp. 135-140 ◽  
Author(s):  
A. Szalay ◽  
D. Munsche ◽  
R. Wollgiehn ◽  
B. Parthier
Keyword(s):  
Ribonucleic Acid ◽  
Extraction Procedure ◽  
Anacystis Nidulans ◽  
Pulse Labelling ◽  
Blue Green Algae ◽  
Molecular Weights ◽  
Close Relationship ◽  
Rrna Species ◽  
Precursor Rna ◽  
Ribosomal Precursor

The RNA of the blue–green alga Anacystis nidulans contains three ribosomal RNA species with molecular weights of 0.56×106, 0.9×106, and 1.1×106if the RNA is extracted in the absence of Mg2+. The 0.9×106mol.wt. rRNA is extremely slowly labelled in32P-incorporation experiments. This rRNA may be a cleavage product of the 1.1×106mol.wt. rRNA from the ribosomes of cells in certain physiological states (e.g. light-deficiency during growth). The cleavage of the 1.1×106mol.wt. rRNA during the extraction procedure can be prevented by the addition of 10mm-MgCl2.32P-pulse-labelling studies demonstrate the rapid synthesis of two ribosomal precursor RNA species. One precursor RNA migrating slightly slower than the 1.1×106mol.wt. rRNA appears much less stable than the other precursor RNA, which shows the electrophoretic behaviour of the 0.7×106mol.wt. rRNA. Our observations support the close relationship between bacteria and blue–green algae also with respect to rRNA maturation. The conversion of the ribosomal precursor RNA species into 0.56×106- and 1.1×106-mol.wt. rRNA species requires Mg2+in the incubation medium.

Impaired Processing of Ribosomal Precursor RNA in Blast Cells of Acute Leukemia

1971 ◽  
Vol 45 (3) ◽  
pp. 201-208 ◽  
Author(s):  
U.L. Torelli ◽  
G.M. Torelli ◽  
A. Andreoli ◽  
C. Mauri
Keyword(s):  
Acute Leukemia ◽  
Blast Cells ◽  
Precursor Rna ◽  
Ribosomal Precursor

scholarly journals NUCLEAR ENVELOPE-ASSOCIATED RESUMPTION OF RNA SYNTHESIS IN LATE MITOSIS OF HELA CELLS

1973 ◽  
Vol 59 (1) ◽  
pp. 150-164 ◽  
Author(s):  
T. Simmons ◽  
P. Heywood ◽  
L. Hodge
Keyword(s):  
Protein Synthesis ◽  
Nuclear Envelope ◽  
Rna Synthesis ◽  
Electron Microscope Autoradiography ◽  
Molecular Synthesis ◽  
Hela S3 ◽  
Precursor Rna ◽  
Ribosomal Precursor ◽  
Hela S3 Cells ◽  
Silver Grains

The restitution of RNA synthesis in cultures progressing from metaphase into interphase (G1) has been investigated in synchronized HeLa S3 cells by using inhibitors of macro-molecular synthesis and the technique of electron microscope autoradiography. The rate of incorporation of radioactive uridine into RNA approached interphase levels in the absence of renewed protein synthesis. In contrast, maintenance of this rate in G1 was dependent upon renewed protein synthesis. Restoration of synthesis of heterogeneous nuclear RNA occurred under conditions that inhibited production of ribosomal precursor RNA. In autoradiographs of individual cells exposed to radioactive uridine, silver grains were first detected after nuclear envelope reformation at the periphery of the chromosome mass but before chromosomal decondensation. These data are consistent with the following interpretation. Multiple RNA polymerase activities persist through mitosis and are involved in the initiation of RNA synthesis in early telophase at sites on the nuclear envelope.

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