Structural comparison of 37-S and 32-S ribosomal precursor RNA in yeast with the mature ribosomal RNA components

1973 ◽  
Vol 294 (3) ◽  
pp. 464-471 ◽  
Author(s):  
R.C. Van Den Bos ◽  
R.J. Planta
Keyword(s):  
Ribosomal Rna ◽  
Structural Comparison ◽  
Precursor Rna ◽  
Ribosomal Precursor

scholarly journals METABOLISM OF RIBOSOMAL PRECURSOR RIBONUCLEIC ACID IN KIDNEY

1970 ◽  
Vol 46 (2) ◽  
pp. 362-369 ◽  
Author(s):  
Geert Ab ◽  
Ronald A. Malt
Keyword(s):  
Ribosomal Rna ◽  
Intermediate Product ◽  
Turnover Time ◽  
Mouse Kidney ◽  
Rnase Inhibitor ◽  
Phenol Extraction ◽  
Precursor Rna ◽  
Ribosomal Precursor ◽  
Kinetics Of ◽  
Rna Precursor

The labile precursors of ribosomal RNA in mouse kidney are preserved when nuclei rapidly isolated after sieving through multiple screens are swollen and cleansed in the presence of an RNase inhibitor before digestion with DNase and phenol extraction. The kinetics of nucleolar labeling analyzed on polyacrylamide gels show that 36S RNA is the major intermediate product in the catabolism of the original 45S RNA precursor to 32S RNA, from which 28S RNA is derived. Each kidney nucleus contains about 200–600 molecules of 45S RNA; the turnover time of the 45S pool is about 3 ± 2 min. Compared with HeLa cells, kidney nuclei have a different major intermediate product and a much smaller and more rapidly turning-over pool of ribosomal precursor RNA.

scholarly journals THE EFFECT OF PUROMYCIN ON INTRANUCLEAR STEPS IN RIBOSOME BIOSYNTHESIS

1968 ◽  
Vol 36 (1) ◽  
pp. 91-101 ◽  
Author(s):  
R. Soeiro ◽  
M. H. Vaughan ◽  
J. E. Darnell
Keyword(s):  
Protein Synthesis ◽  
Ribosomal Rna ◽  
Cell Fractionation ◽  
Sequence Of Events ◽  
Inhibition Of Protein Synthesis ◽  
Precursor Rna ◽  
Initial Processing ◽  
Inhibitor Of Protein Synthesis ◽  
Ribosomal Precursor ◽  
Ribosome Biosynthesis

Inhibition of protein synthesis by puromycin (100 γ/ml) is known to inhibit the synthesis of ribosomes. However, ribosomal precursor RNA (45S) continues to be synthesized, methylated, and processed. Cell fractionation studies revealed that, although the initial processing (45S → 32S + 16S) occurs in the presence of puromycin, the 16S moiety is immediately degraded. No species of ribosomal RNA can be found to have emerged from the nucleolus. The RNA formed in the presence of puromycin is normal as judged by its ability to enter new ribosomal particles after puromycin is removed. This sequence of events is not a result of inhibition of protein synthesis, for cycloheximide, another inhibitor of protein synthesis, either alone or in combination with puromycin allows the completion of new ribosomes.

Structural Comparison of 26S and 17S Ribosomal RNA of Yeast

Nature ◽  
1970 ◽  
Vol 225 (5228) ◽  
pp. 183-184 ◽  
Author(s):  
R. C. VAN DEN BOS ◽  
R. J. PLANTA
Keyword(s):  
Ribosomal Rna ◽  
Structural Comparison

scholarly journals Ribosomal ribonucleic acid and ribosomal precursor ribonucleic acid in Anacystis nidulans

1972 ◽  
Vol 129 (1) ◽  
pp. 135-140 ◽  
Author(s):  
A. Szalay ◽  
D. Munsche ◽  
R. Wollgiehn ◽  
B. Parthier
Keyword(s):  
Ribonucleic Acid ◽  
Extraction Procedure ◽  
Anacystis Nidulans ◽  
Pulse Labelling ◽  
Blue Green Algae ◽  
Molecular Weights ◽  
Close Relationship ◽  
Rrna Species ◽  
Precursor Rna ◽  
Ribosomal Precursor

The RNA of the blue–green alga Anacystis nidulans contains three ribosomal RNA species with molecular weights of 0.56×106, 0.9×106, and 1.1×106if the RNA is extracted in the absence of Mg2+. The 0.9×106mol.wt. rRNA is extremely slowly labelled in32P-incorporation experiments. This rRNA may be a cleavage product of the 1.1×106mol.wt. rRNA from the ribosomes of cells in certain physiological states (e.g. light-deficiency during growth). The cleavage of the 1.1×106mol.wt. rRNA during the extraction procedure can be prevented by the addition of 10mm-MgCl2.32P-pulse-labelling studies demonstrate the rapid synthesis of two ribosomal precursor RNA species. One precursor RNA migrating slightly slower than the 1.1×106mol.wt. rRNA appears much less stable than the other precursor RNA, which shows the electrophoretic behaviour of the 0.7×106mol.wt. rRNA. Our observations support the close relationship between bacteria and blue–green algae also with respect to rRNA maturation. The conversion of the ribosomal precursor RNA species into 0.56×106- and 1.1×106-mol.wt. rRNA species requires Mg2+in the incubation medium.

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