Chimeric Enzyme Composed of Polyhydroxyalkanoate (PHA) Synthases from Ralstonia eutropha and Aeromonas caviae Enhances Production of PHAs in Recombinant Escherichia coli

2009 ◽  
Vol 10 (4) ◽  
pp. 682-685 ◽  
Author(s):  
Ken’ichiro Matsumoto ◽  
Kazuma Takase ◽  
Yoko Yamamoto ◽  
Yoshiharu Doi ◽  
Seiichi Taguchi
Keyword(s):  
Escherichia Coli ◽  
Ralstonia Eutropha ◽  
Recombinant Escherichia Coli ◽  
Chimeric Enzyme ◽  
Aeromonas Caviae

scholarly journals Conversion of Glycerol to Poly(3-Hydroxypropionate) in Recombinant Escherichia coli

2009 ◽  
Vol 76 (2) ◽  
pp. 622-626 ◽  
Author(s):  
Björn Andreeßen ◽  
Alvin Brian Lange ◽  
Horst Robenek ◽  
Alexander Steinbüchel
Keyword(s):  
Escherichia Coli ◽  
Batch Fermentation ◽  
Ralstonia Eutropha ◽  
Dry Weight ◽  
Recombinant Escherichia Coli ◽  
Pha Synthase ◽  
Fed Batch ◽  
Interesting Application ◽  
Serovar Typhimurium ◽  
Fed Batch Fermentation

ABSTRACT We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase (dhaB1) of Clostridium butyricum, propionaldehyde dehydrogenase (pduP) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase (phaC1) of Ralstonia eutropha were expressed in recombinant Escherichia coli. Poly(3HP) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. The present study shows an interesting application to engineer a poly(3HP) synthesis pathway in bacteria.

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