Thermodynamics of ionization of deuterium oxide

1968 ◽  
Vol 72 (13) ◽  
pp. 4654-4659 ◽  
Author(s):  
Robert N. Goldberg ◽  
Loren G. Hepler
Keyword(s):  
Deuterium Oxide ◽  
Thermodynamics Of Ionization

scholarly journals Water and Deuterium Oxide Permeability through Aquaporin 1: MD Predictions and Experimental Verification

2007 ◽  
Vol 130 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Artem B. Mamonov ◽  
Rob D. Coalson ◽  
Mark L. Zeidel ◽  
John C. Mathai
Keyword(s):  
Deuterium Oxide ◽  
Diffusion Constant ◽  
Structural Data ◽  
Md Simulations ◽  
Water Model ◽  
Aquaporin 1 ◽  
Self Diffusion ◽  
Osmotic Permeability ◽  
Protein Channels ◽  
And Diffusion

Determining the mechanisms of flux through protein channels requires a combination of structural data, permeability measurement, and molecular dynamics (MD) simulations. To further clarify the mechanism of flux through aquaporin 1 (AQP1), osmotic pf (cm3/s/pore) and diffusion pd (cm3/s/pore) permeability coefficients per pore of H2O and D2O in AQP1 were calculated using MD simulations. We then compared the simulation results with experimental measurements of the osmotic AQP1 permeabilities of H2O and D2O. In this manner we evaluated the ability of MD simulations to predict actual flux results. For the MD simulations, the force field parameters of the D2O model were reparameterized from the TIP3P water model to reproduce the experimentally observed difference in the bulk self diffusion constants of H2O vs. D2O. Two MD systems (one for each solvent) were constructed, each containing explicit palmitoyl-oleoyl-phosphatidyl-ethanolamine (POPE) phospholipid molecules, solvent, and AQP1. It was found that the calculated value of pf for D2O is ∼15% smaller than for H2O. Bovine AQP1 was reconstituted into palmitoyl-oleoyl-phosphatidylcholine (POPC) liposomes, and it was found that the measured macroscopic osmotic permeability coefficient Pf (cm/s) of D2O is ∼21% lower than for H2O. The combined computational and experimental results suggest that deuterium oxide permeability through AQP1 is similar to that of water. The slightly lower observed osmotic permeability of D2O compared to H2O in AQP1 is most likely due to the lower self diffusion constant of D2O.

scholarly journals Deuterium Oxide (D2O) Induces Early Stress Response Gene Expression and Impairs Growth and Metastasis of Experimental Malignant Melanoma

Cancers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 605
Author(s):  
Jana Jandova ◽  
Anh B. Hua ◽  
Jocelyn Fimbres ◽  
Georg T. Wondrak
Keyword(s):  
Gene Expression ◽  
Stress Response ◽  
Malignant Melanoma ◽  
Tumor Growth ◽  
Deuterium Oxide ◽  
Human Melanoma ◽  
Pharmacological Intervention ◽  
Melanoma Metastasis ◽  
Response Gene ◽  
Stress Response Gene

There are two stable isotopes of hydrogen, protium (1H) and deuterium (2H; D). Cellular stress response dysregulation in cancer represents both a major pathological driving force and a promising therapeutic target, but the molecular consequences and potential therapeutic impact of deuterium (2H)-stress on cancer cells remain largely unexplored. We have examined the anti-proliferative and apoptogenic effects of deuterium oxide (D2O; ‘heavy water’) together with stress response gene expression profiling in panels of malignant melanoma (A375V600E, A375NRAS, G361, LOX-IMVI), and pancreatic ductal adenocarcinoma (PANC-1, Capan-2, or MIA PaCa-2) cells with inclusion of human diploid Hs27 skin fibroblasts. Moreover, we have examined the efficacy of D2O-based pharmacological intervention in murine models of human melanoma tumor growth and metastasis. D2O-induction of apoptosis was substantiated by AV-PI flow cytometry, immunodetection of PARP-1, and pro-caspase 3 cleavage, and rescue by pan-caspase inhibition. Differential array analysis revealed early modulation of stress response gene expression in both A375 melanoma and PANC-1 adenocarcinoma cells elicited by D2O (90%; ≤6 h) (upregulated: CDKN1A, DDIT3, EGR1, GADD45A, HMOX1, NFKBIA, or SOD2 (up to 9-fold; p < 0.01)) confirmed by independent RT-qPCR analysis. Immunoblot analysis revealed rapid onset of D2O-induced stress response phospho-protein activation (p-ERK, p-JNK, p-eIF2α, or p-H2AX) or attenuation (p-AKT). Feasibility of D2O-based chemotherapeutic intervention (drinking water (30% w/w)) was demonstrated in a severe combined immunodeficiency (SCID) mouse melanoma metastasis model using luciferase-expressing A375-Luc2 cells. Lung tumor burden (visualized by bioluminescence imaging) was attenuated by D2O, and inhibition of invasiveness was also confirmed in an in vitro Matrigel transwell invasion assay. D2O supplementation also suppressed tumor growth in a murine xenograft model of human melanoma, and median survival was significantly increased without causing adverse effects. These data demonstrate for the first time that systemic D2O administration impairs growth and metastasis of malignant melanoma through the pharmacological induction of deuterium (2H)-stress.

Action of deuterium oxide upon the ERG circadian rhythm in crayfish, Procambarus bouvieri

1988 ◽  
Vol 90 (3) ◽  
pp. 435-440 ◽  
Author(s):  
Beatriz Fuentes Pardo ◽  
Enrique Moreno Sáenz
Keyword(s):  
Circadian Rhythm ◽  
Deuterium Oxide

Precise measurement of total body water using trace quantities of deuterium oxide

1977 ◽  
Vol 4 (2) ◽  
pp. 82-87 ◽  
Author(s):  
D. Halliday ◽  
A. G. Miller
Keyword(s):  
Total Body Water ◽  
Precise Measurement ◽  
Deuterium Oxide ◽  
Body Water ◽  
Total Body

ChemInform Abstract: Reductive Dechlorination of Chloro-phenols and -benzoic Acids by Raney Co-Al Alloy in an Alkaline Deuterium Oxide Solution and Preparation of Deuteriated Salicylic Acids.

ChemInform ◽  
1990 ◽  
Vol 21 (23) ◽  
Author(s):  
M. TASHIRO ◽  
H. TSUZUKI ◽  
J. MATSUMOTO ◽  
S. MATAKA ◽  
K. NAKAYAMA ◽  
...  
Keyword(s):  
Reductive Dechlorination ◽  
Deuterium Oxide ◽  
Al Alloy ◽  
Benzoic Acids ◽  
Salicylic Acids

Effects of acute hypobaric hypoxia on the appearance of ingested deuterium from a deuterium oxide-labelled carbohydrate beverage in body fluids of humans during prolonged cycling exercise

1999 ◽  
Vol 79 (5) ◽  
pp. 397-403 ◽  
Author(s):  
N. Koulmann ◽  
B. Melin ◽  
L. Bourdon ◽  
F. P�ronnet ◽  
C. Jimenez ◽  
...  
Keyword(s):  
Hypobaric Hypoxia ◽  
Deuterium Oxide ◽  
Body Fluids ◽  
Acute Hypobaric Hypoxia ◽  
Cycling Exercise

A comparison of the phase transition properties of 1,2-diacylphosphatidylcholines and 1,2-diacylphosphatidylethanolamines in water and deuterium oxide

1984 ◽  
Vol 88 (22) ◽  
pp. 5401-5406 ◽  
Author(s):  
Gert Lipka ◽  
Babur Z. Chowdhry ◽  
Julian M. Sturtevant
Keyword(s):  
Phase Transition ◽  
Deuterium Oxide

scholarly journals Influence of deuterium oxide on calcium transients and myofibrillar responses of frog skeletal muscle.

1984 ◽  
Vol 354 (1) ◽  
pp. 225-251 ◽  
Author(s):  
D G Allen ◽  
J R Blinks ◽  
R E Godt
Keyword(s):  
Skeletal Muscle ◽  
Deuterium Oxide ◽  
Calcium Transients ◽  
Frog Skeletal Muscle

The permeability of living cells to heavy water (deuterium oxide)

1935 ◽  
Vol 5 (4) ◽  
pp. 473-482 ◽  
Author(s):  
Balduin Lucké ◽  
E. Newton Harvey
Keyword(s):  
Heavy Water ◽  
Deuterium Oxide ◽  
Living Cells
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