Substrate specificity of enzymes in organic solvents vs. water is reversed

1986 ◽  
Vol 108 (10) ◽  
pp. 2767-2768 ◽  
Author(s):  
Aleksey. Zaks ◽  
Alexander M. Klibanov
Keyword(s):  
Substrate Specificity ◽  
Organic Solvents

Control of Subtilisin Substrate Specificity by Solvent Engineering in Organic Solvents and Supercritical Fluoroform

1996 ◽  
Vol 118 (51) ◽  
pp. 12891-12901 ◽  
Author(s):  
Apurva K. Chaudhary ◽  
Sanjay V. Kamat ◽  
Eric J. Beckman ◽  
David Nurok ◽  
Robert M. Kleyle ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Organic Solvents ◽  
Solvent Engineering

scholarly journals Characterization of a Thermostable Short-Chain Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Thermococcus sibiricus

2010 ◽  
Vol 76 (12) ◽  
pp. 4096-4098 ◽  
Author(s):  
Tatiana N. Stekhanova ◽  
Andrey V. Mardanov ◽  
Ekaterina Y. Bezsudnova ◽  
Vadim M. Gumerov ◽  
Nikolai V. Ravin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Alcohol Dehydrogenase ◽  
Substrate Specificity ◽  
Organic Solvents ◽  
Short Chain ◽  
Chain Alcohol ◽  
Hyperthermophilic Archaeon ◽  
Broad Substrate Specificity ◽  
Short Chain Alcohol

ABSTRACT Short-chain alcohol dehydrogenase, encoded by the gene Tsib_0319 from the hyperthermophilic archaeon Thermococcus sibiricus, was expressed in Escherichia coli, purified and characterized as an NADPH-dependent enantioselective oxidoreductase with broad substrate specificity. The enzyme exhibits extremely high thermophilicity, thermostability, and tolerance to organic solvents and salts.

Application of Thermostable Lipolytic Bacterial Enzymes for Modern Biotechnological Processes: Review

2018 ◽  
Vol 18 (6) ◽  
pp. 61-73 ◽  
Author(s):  
Yu. V. Samoilova ◽  
K. N. Sorokina ◽  
A. V. Piligaev ◽  
V. N. Parmon
Keyword(s):  
Wastewater Treatment ◽  
Substrate Specificity ◽  
Organic Solvents ◽  
Food Industry ◽  
Review Paper ◽  
Chemical Compounds ◽  
Functional Stability ◽  
Bacterial Enzymes ◽  
Biotechnological Processes

In the review paper, the modern investigations on the application of thermostable lipolytic bacterial enzymes for biotechnology are discussed, the properties of these enzymes discussed including their activity and functional stability at various temperatures, pH in organic solvents, as well as the substrate specificity and activity in the presence of various chemical compounds. The paper contains data on the development of recombinant producers of lipolytic bacterial enzymes and on approaches to improving their productivity. The application of the bacterial lipases for biotechnological processes of synthesis of biofuel, various chemicals and detergents, for food industry and wastewater treatment is considered.

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