Determination of rotational correlation times of proteins in solution from carbon-13 spin-lattice relaxation measurements. Effect of magnetic field strength and anisotropic rotation

1976 ◽  
Vol 98 (25) ◽  
pp. 8250-8254 ◽  
Author(s):  
David J. Wilbur ◽  
Raymond S. Norton ◽  
Arthur O. Clouse ◽  
Robert Addleman ◽  
Adam Allerhand
Keyword(s):  
Magnetic Field ◽  
Field Strength ◽  
Magnetic Field Strength ◽  
Lattice Relaxation ◽  
Spin Lattice Relaxation ◽  
Correlation Times ◽  
Spin Lattice ◽  
Relaxation Measurements ◽  
Rotational Correlation Times

scholarly journals Effect of magnetic field strength on the linewidth and spin-lattice relaxation time of the thiocyanate carbon of cyanylated β-lactoglobulin B: optimization of the experimental parameters for observing thiocyanate carbons in proteins

1995 ◽  
Vol 306 (2) ◽  
pp. 531-535
Author(s):  
J P G Malthouse ◽  
P Phelan
Keyword(s):  
Magnetic Field ◽  
Field Strength ◽  
Magnetic Field Strength ◽  
Chemical Shift Anisotropy ◽  
Relaxation Times ◽  
Lattice Relaxation ◽  
Spin Lattice Relaxation ◽  
Spin Lattice Relaxation Time ◽  
Spin Lattice ◽  
Field Strengths

The linewidths and spin-lattice relaxation times of the 13C-n.m.r. signal at 109.7 p.p.m. due to the thiocyanate carbon of intact [cyanato-13C]cyanylated-beta-lactoglobulin-B have been determined at magnetic field strengths of 1.88, 6.34 and 11.74 T as well as the spin-lattice relaxation times of its backbone alpha-carbon atoms. The linewidths were directly proportional to the square of the magnetic field strength and we conclude that, at magnetic field strengths of 6.34 T or above, more than 70% of the linewidth will be determined by chemical-shift anisotropy. We estimate that the spin-lattice relaxation time resulting from the chemical-shift anisotropy of the thiocyanate carbon is 1.52 +/- 0.1 s and we conclude that for magnetic field strengths of 6.34 T and above the observed spin-lattice relaxation time of the thiocyanate carbon will be essentially independent of magnetic field strength. Using the rigid-rotor model we obtain estimates of the rotational correlation time of [cyanato-13C]cyanylated-beta-lactoglobulin-B and of the chemical-shift anisotropy shielding tensor of its thiocyanate carbon. We have calculated the linewidths and spin-lattice relaxation times of thiocyanate carbons at magnetic field strengths of 1.88-14.1 T in proteins with M(r) values in the range 10,000-400,000. The effects of magnetic field strength on the resolution and signal-to-noise ratios of the signals due to thiocyanate carbons attached to proteins of M(r) greater than 10,000 are discussed.

scholarly journals 19F spin–lattice relaxation of perfluoropolyethers: Dependence on temperature and magnetic field strength (7.0–14.1T)

2014 ◽  
Vol 242 ◽  
pp. 18-22 ◽  
Author(s):  
Deepak K. Kadayakkara ◽  
Krishnan Damodaran ◽  
T. Kevin Hitchens ◽  
Jeff W.M. Bulte ◽  
Eric T. Ahrens
Keyword(s):  
Magnetic Field ◽  
Field Strength ◽  
Magnetic Field Strength ◽  
Lattice Relaxation ◽  
Spin Lattice Relaxation ◽  
Spin Lattice

31P Spin-Lattice Relaxation Times and Resonance Linewidths of Rat Tissuein Vivo: Dependence upon the Static Magnetic Field Strength

1985 ◽  
Vol 2 (4) ◽  
pp. 410-417 ◽  
Author(s):  
Jeffrey L. Evelhoch ◽  
Coleen S. Ewy ◽  
Barry A. Siegfried ◽  
Joseph J. H. Ackerman ◽  
David W. Rice ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Field Strength ◽  
Magnetic Field Strength ◽  
Static Magnetic Field ◽  
Relaxation Times ◽  
Lattice Relaxation ◽  
Spin Lattice Relaxation ◽  
Spin Lattice

scholarly journals Determination of intramolecular microdynamical characteristics in solution of the cyclic hexadepsipeptide antibiotic pristinamycin IA from13C spin lattice relaxation measurements

Biopolymers ◽  
1982 ◽  
Vol 21 (5) ◽  
pp. 1005-1009 ◽  
Author(s):  
R. E. A. Callens ◽  
M. J. O. Anteunis ◽  
F. A. M. Borremans
Keyword(s):  
Lattice Relaxation ◽  
Spin Lattice Relaxation ◽  
Spin Lattice ◽  
Relaxation Measurements

Rotational correlation times for ethanol by deuteron magnetic spin–lattice relaxation

1975 ◽  
Vol 63 (7) ◽  
pp. 3176 ◽  
Author(s):  
Teng ko Chen ◽  
A. L. Beyerlein ◽  
G. B. Savitsky
Keyword(s):  
Lattice Relaxation ◽  
Spin Lattice Relaxation ◽  
Correlation Times ◽  
Spin Lattice ◽  
Rotational Correlation Times ◽  
Magnetic Spin ◽  
Rotational Correlation
Sign in / Sign up

Export Citation Format

Share Document