Genetic Engineering of the Heme Pocket in Human Serum Albumin:  Modulation of O2Binding of Iron Protoporphyrin IX by Variation of Distal Amino Acids

2007 ◽  
Vol 129 (36) ◽  
pp. 11286-11295 ◽  
Author(s):  
Teruyuki Komatsu ◽  
Akito Nakagawa ◽  
Patricia A. Zunszain ◽  
Stephen Curry ◽  
Eishun Tsuchida
Keyword(s):  
Amino Acids ◽  
Human Serum Albumin ◽  
Genetic Engineering ◽  
Serum Albumin ◽  
Human Serum ◽  
Protoporphyrin Ix ◽  
Heme Pocket ◽  
Iron Protoporphyrin

The role of an amino acid triad at the entrance of the heme pocket in human serum albumin for O2 and CO binding to iron protoporphyrin IX

2009 ◽  
Vol 7 (18) ◽  
pp. 3836 ◽  
Author(s):  
Teruyuki Komatsu ◽  
Akito Nakagawa ◽  
Stephen Curry ◽  
Eishun Tsuchida ◽  
Kenichi Murata ◽  
...  
Keyword(s):  
Amino Acid ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Protoporphyrin Ix ◽  
Heme Pocket ◽  
Iron Protoporphyrin

O2and CO Binding Properties of Artificial Hemoproteins Formed by Complexing Iron Protoporphyrin IX with Human Serum Albumin Mutants

2005 ◽  
Vol 127 (45) ◽  
pp. 15933-15942 ◽  
Author(s):  
Teruyuki Komatsu ◽  
Naomi Ohmichi ◽  
Akito Nakagawa ◽  
Patricia A. Zunszain ◽  
Stephen Curry ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Protoporphyrin Ix ◽  
Binding Properties ◽  
Iron Protoporphyrin

O2Binding Properties of Human Serum Albumin Quadruple Mutant Complexed Iron Protoporphyrin IX with Axial His-186 Coordination

2009 ◽  
Vol 38 (8) ◽  
pp. 776-777 ◽  
Author(s):  
Akito Nakagawa ◽  
Teruyuki Komatsu ◽  
Stephen Curry ◽  
Eishun Tsuchida
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Protoporphyrin Ix ◽  
Quadruple Mutant ◽  
Iron Protoporphyrin

In vitro stereoselective degradation of carprofen glucuronide by human serum albumin. Characterization of sites and reactive amino acids

Chirality ◽  
2000 ◽  
Vol 12 (2) ◽  
pp. 53-62 ◽  
Author(s):  
H�l�ne Georges ◽  
Nathalie Presle ◽  
Thierry Buronfosse ◽  
Sylvie Fournel-Gigleux ◽  
Patrick Netter ◽  
...  
Keyword(s):  
Amino Acids ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Stereoselective Degradation

Exploring the Molecular Level Interaction of Human Serum Albumin with Calcium Oxalate Monohydrate Crystals

2021 ◽  
Vol 28 ◽  
Author(s):  
Priyadarshini ◽  
Abhishek Negi ◽  
Chetna Faujdar ◽  
Lokesh Nigam ◽  
Naidu Subbarao
Keyword(s):  
Amino Acids ◽  
Crystal Growth ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Calcium Oxalate ◽  
Human Serum ◽  
Stone Formation ◽  
Calcium Oxalate Monohydrate ◽  
Calcium Oxalate Crystallization ◽  
In Silico Docking

Background: Human serum albumin (HSA) is one of the most abundant proteins in the blood plasma, urine as well as in the organic matrix of renal calculi. Macromolecules present in the urine modulate kidney stone formation either by stimulating or inhibiting crystallization process. Objective: In the present study, effect of HSA protein on the growth of calcium oxalate monohydrate crystal (COM) was investigated. Methods: Crystal growth assay was used to measure oxalate depletion in the crystal seeded solution in the presence of HSA. HSA concentrations exhibiting effect on crystal growth were selected for FTIR and XRD analysis. In silico docking was performed on seven different binding sites of HSA. Results: Albumin is playing dual role in growth of calcium oxalate crystallization. FTIR and XRD studies further revealed HSA exerted strain over crystal thus affecting its structure by interacting with amino acids of its pocket 1. Docking results indicate that out of 7 binding pocket in protein, calcium oxalate interacts with Arg-186 and Lys-190 amino acids of pocket 1. Conclusion: Our study confirms the role of HSA in calcium oxalate crystallization where acidic amino acids arginine and lysine are binding with COM crystals, revealing molecular interaction of macromolecule and crystal in urolithiasis.

Dioxygenation of Human Serum Albumin Having a Prosthetic Heme Group in a Tailor-Made Heme Pocket

2004 ◽  
Vol 126 (44) ◽  
pp. 14304-14305 ◽  
Author(s):  
Teruyuki Komatsu ◽  
Naomi Ohmichi ◽  
Patricia A. Zunszain ◽  
Stephen Curry ◽  
Eishun Tsuchida
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Heme Group ◽  
Heme Pocket ◽  
Prosthetic Heme Group
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