Quantitation of Affinity, Avidity, and Binding Kinetics of Protein Analytes with a Dynamically Switchable Biosurface

2012 ◽  
Vol 134 (37) ◽  
pp. 15225-15228 ◽  
Author(s):  
Jelena Knezevic ◽  
Andreas Langer ◽  
Paul A. Hampel ◽  
Wolfgang Kaiser ◽  
Ralf Strasser ◽  
...  
Keyword(s):  
Binding Kinetics ◽  
Kinetics Of ◽  
Protein Analytes

Binding kinetics of fluorescent bisphosphonates as a tool for monitoring bone dynamics in vivo

2013 ◽  
Author(s):  
Robert Tower ◽  
Graeme Campbell ◽  
Marc Muller ◽  
Olga Will ◽  
Frederieka Grundmann ◽  
...  
Keyword(s):  
Binding Kinetics ◽  
Kinetics Of ◽  
Bone Dynamics

Influence of Brain Gangliosides on the Formation and Properties of Supported Lipid Bilayers for Binding Kinetics Studies

2018 ◽  
Author(s):  
Luke Jordan ◽  
Nathan Wittenberg
Keyword(s):  
Lipid Bilayers ◽  
Binding Kinetics ◽  
Supported Lipid Bilayers ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Supported Lipid Bilayer ◽  
Head Groups ◽  
Lipid Diffusion ◽  
Kinetics Of ◽  
Brain Gangliosides

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.

scholarly journals CO Binding Kinetics of Human Cytochrome P450 3A4

1995 ◽  
Vol 270 (10) ◽  
pp. 5014-5018 ◽  
Author(s):  
Aditya P. Koley ◽  
Jeroen T. M. Buters ◽  
Richard C. Robinson ◽  
Allen Markowitz ◽  
Fred K. Friedman
Keyword(s):  
Cytochrome P450 ◽  
Binding Kinetics ◽  
Cytochrome P450 3A4 ◽  
Human Cytochrome ◽  
Kinetics Of

scholarly journals Measuring microtubule binding kinetics of membrane-bound kinesin motors using supported lipid bilayers

2021 ◽  
Vol 2 (3) ◽  
pp. 100691
Author(s):  
Rui Jiang ◽  
William O. Hancock
Keyword(s):  
Lipid Bilayers ◽  
Binding Kinetics ◽  
Supported Lipid Bilayers ◽  
Microtubule Binding ◽  
Membrane Bound ◽  
Kinetics Of

scholarly journals Dissecting the impact of target-binding kinetics of protein binders on tumor localization

iScience ◽  
2021 ◽  
Vol 24 (2) ◽  
pp. 102104
Author(s):  
Yunjin Song ◽  
Hoibin Jeong ◽  
Song-Rae Kim ◽  
Yiseul Ryu ◽  
Jonghwi Baek ◽  
...  
Keyword(s):  
Binding Kinetics ◽  
Tumor Localization ◽  
Protein Binders ◽  
Target Binding ◽  
The Impact ◽  
Kinetics Of

Isolation, Characterization, and DNA Binding Kinetics of Three Dirhodium(II,II) Carboxyamidate Complexes: Rh2(μ-L)(HNOCCF3)3where L= [OOCCH3]−, [OOCCF3]−, or [HNOCCF3]−

2011 ◽  
Vol 50 (8) ◽  
pp. 3458-3463 ◽  
Author(s):  
Shari U. Dunham ◽  
Todd S. Remaley ◽  
Bryn S. Moore ◽  
Debra L. Evans ◽  
Stephen U. Dunham
Keyword(s):  
Dna Binding ◽  
Binding Kinetics ◽  
Kinetics Of

Halide and Proton Binding Kinetics of Yellow Fluorescent Protein Variants

Biochemistry ◽  
2013 ◽  
Vol 52 (14) ◽  
pp. 2482-2491 ◽  
Author(s):  
Harriet E. Seward ◽  
Jaswir Basran ◽  
Roanne Denton ◽  
Mark Pfuhl ◽  
Frederick W. Muskett ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Yellow Fluorescent Protein ◽  
Binding Kinetics ◽  
Proton Binding ◽  
Protein Variants ◽  
Kinetics Of

scholarly journals Real-time reliable determination of binding kinetics of DNA hybridization using a multi-channel graphene biosensor

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Shicai Xu ◽  
Jian Zhan ◽  
Baoyuan Man ◽  
Shouzhen Jiang ◽  
Weiwei Yue ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Hybridization ◽  
Binding Kinetics ◽  
Reliable Determination ◽  
Kinetics Of
Sign in / Sign up

Export Citation Format

Share Document