Spectral Tuning of Ultraviolet Cone Pigments: An Interhelical Lock Mechanism

Sivakumar Sekharan; Victoria L. Mooney; Ivan Rivalta; Manija A. Kazmi; Maureen Neitz; Jay Neitz; Thomas P. Sakmar; Elsa C. Y. Yan; Victor S. Batista

doi:10.1021/ja409896y

Spectral Tuning of Ultraviolet Cone Pigments: An Interhelical Lock Mechanism

Journal of the American Chemical Society ◽

10.1021/ja409896y ◽

2013 ◽

Vol 135 (51) ◽

pp. 19064-19067 ◽

Cited By ~ 18

Author(s):

Sivakumar Sekharan ◽

Victoria L. Mooney ◽

Ivan Rivalta ◽

Manija A. Kazmi ◽

Maureen Neitz ◽

...

Keyword(s):

Spectral Tuning ◽

Cone Pigments

Evolutionary analysis of rhodopsin and cone pigments: connecting the three-dimensional structure with spectral tuning and signal transfer

FEBS Letters ◽

10.1016/s0014-5793(03)01152-9 ◽

2003 ◽

Vol 555 (1) ◽

pp. 151-159 ◽

Cited By ~ 11

Author(s):

David C. Teller ◽

Ronald E. Stenkamp ◽

Krzysztof Palczewski

Keyword(s):

Three Dimensional ◽

Dimensional Structure ◽

Evolutionary Analysis ◽

Spectral Tuning ◽

Signal Transfer ◽

Three Dimensional Structure ◽

Cone Pigments

Spectral Tuning of Rhodopsin and Visual Cone Pigments

Journal of the American Chemical Society ◽

10.1021/ja411864m ◽

2014 ◽

Vol 136 (7) ◽

pp. 2723-2726 ◽

Cited By ~ 32

Author(s):

Xiuwen Zhou ◽

Dage Sundholm ◽

Tomasz A. Wesołowski ◽

Ville R. I. Kaila

Keyword(s):

Spectral Tuning ◽

Cone Pigments

Spectral Tuning of Deep Red Cone Pigments†

Biochemistry ◽

10.1021/bi702069d ◽

2008 ◽

Vol 47 (16) ◽

pp. 4614-4620 ◽

Cited By ~ 10

Author(s):

Tabitha L. Amora ◽

Lavoisier S. Ramos ◽

Jhenny F. Galan ◽

Robert R. Birge

Keyword(s):

Spectral Tuning ◽

Cone Pigments

Spectral Tuning and Evolution of Short Wave-Sensitive Cone Pigments in Cottoid Fish from Lake Baikal†

Biochemistry ◽

10.1021/bi025656e ◽

2002 ◽

Vol 41 (19) ◽

pp. 6019-6025 ◽

Cited By ~ 34

Author(s):

Jill A. Cowing ◽

Subathra Poopalasundaram ◽

Susan E. Wilkie ◽

James K. Bowmaker ◽

David M. Hunt

Keyword(s):

Lake Baikal ◽

Short Wave ◽

Spectral Tuning ◽

Cone Pigments ◽

Cottoid Fish

Spectral Tuning in the Mammalian Short-Wavelength Sensitive Cone Pigments†

Biochemistry ◽

10.1021/bi0200413 ◽

2002 ◽

Vol 41 (21) ◽

pp. 6860-6865 ◽

Cited By ~ 73

Author(s):

Jeffry I. Fasick ◽

Meredithe L. Applebury ◽

Daniel D. Oprian

Keyword(s):

Short Wavelength ◽

Spectral Tuning ◽

Cone Pigments

Vitamin A1/A2 chromophore exchange: its role in spectral tuning and visual plasticity

Developmental Biology ◽

10.1016/j.ydbio.2021.03.002 ◽

2021 ◽

Author(s):

Joseph C. Corbo

Keyword(s):

Spectral Tuning ◽

Visual Plasticity

Spectral tuning of dye doped hollow polymer optical fiber with silver doped micro ring

Optics Communications ◽

10.1016/j.optcom.2021.127134 ◽

2021 ◽

pp. 127134

Author(s):

Arindam Sarkar ◽

V.P.N. Nampoori ◽

M. Kailasnath

Keyword(s):

Optical Fiber ◽

Spectral Tuning ◽

Polymer Optical Fiber

Spectral tuning of double resonant nanolaminate plasmonic nanoantennas with a fixed size

Applied Physics Letters ◽

10.1063/5.0054220 ◽

2021 ◽

Vol 118 (24) ◽

pp. 241108

Author(s):

E. Mejia ◽

Y. Qian ◽

S. A. Safiabadi Tali ◽

J. Song ◽

W. Zhou

Keyword(s):

Spectral Tuning ◽

Fixed Size ◽

Plasmonic Nanoantennas

The molecular mechanisms of light adaption in light-harvesting complexes of purple bacteria revealed by a multiscale modeling

Chemical Science ◽

10.1039/c9sc02886b ◽

2019 ◽

Vol 10 (42) ◽

pp. 9650-9662 ◽

Cited By ~ 8

Author(s):

Felipe Cardoso Ramos ◽

Michele Nottoli ◽

Lorenzo Cupellini ◽

Benedetta Mennucci

Keyword(s):

Multiscale Modeling ◽

Molecular Mechanisms ◽

Light Harvesting ◽

Purple Bacteria ◽

Spectral Tuning ◽

Light Harvesting Complexes

The spectral tuning of LH2 antenna complexes arises from H-bonding, acetyl torsion, and inter-chromophore couplings.

Salamander rods and cones contain distinct transducin alpha subunits

Visual Neuroscience ◽

10.1017/s0952523800176047 ◽

2000 ◽

Vol 17 (6) ◽

pp. 847-854 ◽

Cited By ~ 5

Author(s):

JAMES C. RYAN ◽

SERGEY ZNOIKO ◽

LIN XU ◽

ROSALIE K. CROUCH ◽

JIAN-XING MA

Keyword(s):

Amino Acid ◽

Blot Analysis ◽

Cellular Localization ◽

Amino Acid Level ◽

Single Copy ◽

Lower Vertebrate ◽

Rods And Cones ◽

Sequence Identity ◽

Outer Segments ◽

Cone Pigments

The mammalian retina is known to contain two distinct transducins that interact with their respective rod and cone pigments. However, there are no reports of a nonmammalian species having two distinct transducins. In the present study, we report the cloning and cellular localization of two transducin α subunits (Gαt) from the tiger salamander. Through degenerate polymerase chain reaction (PCR) and subsequent screening of a salamander retina cDNA library, we have identified two forms of Gαt. When compared to existing sequences in GenBank, the cloned subunits showed high similarity to rod and cone transducins. The salamander Gαt-1 has 91.2–93.7% amino acid sequence identity to mammalian rod Gαt subunits and 79.7–80.9% to mammalian cone Gαts. The salamander Gαt-2 has 86.2–87.9% sequence identity to mammalian cone Gαts and 78.9–80.9% to mammalian rod Gαts at the amino acid level. The Gαt-1 cDNA encodes 350 amino acids while the Gαt-2 cDNA encodes 354 residues, which is typical for rod and cone Gαts, respectively, and we thus identified the Gαt-1 as rod and Gαt-2 as cone Gαt. Sequences identified as effector binding sites and GTPase activity regions are highly conserved between the two subunits. Genomic Southern blot analysis showed that rod and cone Gαt subunits are both encoded by single-copy genes. Northern blot analysis identified retina-specific transcripts of 3.0 kb for rod Gαt and 2.6 kb for cone Gαt. Immunohistochemistry in the flat-mounted salamander retina demonstrated that rod Gαt is localized to rods, predominantly in the outer segments; similarly, cone Gαt is localized to cone outer segments. The results confirm that the two sequences encode rod and cone transducins and demonstrate that this lower vertebrate contains two distinct transducins that are localized specifically to rod and cone photoreceptors.