Approaches to the Solution NMR Characterization of Active Sites for 65 kDa Tetrameric Hemoglobins in the Paramagnetic Cyanomet State

1997 ◽  
Vol 119 (51) ◽  
pp. 12643-12654 ◽  
Author(s):  
Urszula Kolczak ◽  
Chang Han ◽  
L. A. Sylvia ◽  
Gerd N. La Mar
Keyword(s):  
Active Sites ◽  
Solution Nmr ◽  
Nmr Characterization

scholarly journals Evidence that the TRPV1 S1-S4 Membrane Domain Contributes to Thermosensing

2019 ◽  
Author(s):  
Minjoo Kim ◽  
Nicholas J. Sisco ◽  
Jacob K. Hilton ◽  
Camila M. Montano ◽  
Manuel A. Castro ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Solution Nmr ◽  
Membrane Domain ◽  
Temperature Dependent ◽  
Nmr Characterization ◽  
Significant Interest ◽  
Pore Domain ◽  
Coupling Mechanisms

AbstractSensing and responding to temperature is crucial in biology. The TRPV1 ion channel is a well-studied heat-sensing receptor that is also activated by vanilloid compounds including capsaicin. Despite significant interest, the molecular underpinnings of thermosensing have remained elusive. The TRPV1 S1-S4 membrane domain couples chemical ligand binding to the pore domain during channel gating. However, the role of the S1-S4 domain in thermosensing is unclear. Evaluation of the isolated human TRPV1 S1-S4 domain by solution NMR, Far-UV CD, and intrinsic fluorescence shows that this domain undergoes a non-denaturing temperature-dependent transition with a high thermosensitivity. Further NMR characterization of the temperature-dependent conformational changes suggests the contribution of the S1-S4 domain to thermosensing shares features with known coupling mechanisms between this domain with ligand and pH activation. Taken together, this study shows that the TRPV1 S1-S4 domain contributes to TRPV1 temperature-dependent activation.

scholarly journals Solution NMR characterization of chemokine CXCL8/IL-8 monomer and dimer binding to glycosaminoglycans: structural plasticity mediates differential binding interactions

2015 ◽  
Vol 472 (1) ◽  
pp. 121-133 ◽  
Author(s):  
Prem Raj B. Joseph ◽  
Philip D. Mosier ◽  
Umesh R. Desai ◽  
Krishna Rajarathnam
Keyword(s):  
Structural Plasticity ◽  
Neutrophil Function ◽  
Solution Nmr ◽  
Binding Interactions ◽  
High Affinity ◽  
Nmr Characterization ◽  
Differential Binding

Structural plasticity plays a major role in determining differential binding of CXCL8 monomer and dimer to glycosaminoglycans (GAGs) and that dimer is the high-affinity GAG ligand. We propose that these properties play important roles in orchestrating in vivo chemokine-mediated neutrophil function.

Solution NMR characterization of apical membrane antigen 1 and small molecule interactions as a basis for designing new antimalarials

2016 ◽  
Vol 29 (6) ◽  
pp. 281-291 ◽  
Author(s):  
Bankala Krishnarjuna ◽  
San Sui Lim ◽  
Shane M. Devine ◽  
Cael O. Debono ◽  
Raymond Lam ◽  
...  
Keyword(s):  
Small Molecule ◽  
Apical Membrane ◽  
Solution Nmr ◽  
Apical Membrane Antigen 1 ◽  
Apical Membrane Antigen ◽  
Nmr Characterization ◽  
Molecule Interactions

Solution NMR characterization of Sgf73(1–104) indicates that Zn ion is required to stabilize zinc finger motif

2010 ◽  
Vol 397 (3) ◽  
pp. 436-440 ◽  
Author(s):  
Chaohua Lai ◽  
Minhao Wu ◽  
Pan Li ◽  
Chaowei Shi ◽  
Changlin Tian ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Solution Nmr ◽  
Zinc Finger Motif ◽  
Nmr Characterization

scholarly journals Solution NMR Characterization of an Unusual Distal H-bond Network in the Active Site of the Cyanide-inhibited, Human Heme Oxygenase Complex of the Symmetric Substrate, 2,4-Dimethyldeuterohemin

2002 ◽  
Vol 277 (36) ◽  
pp. 33018-33031 ◽  
Author(s):  
Yiming Li ◽  
Ray T. Syvitski ◽  
Karine Auclair ◽  
Angela Wilks ◽  
Paul R. Ortiz de Montellano ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Active Site ◽  
Solution Nmr ◽  
Nmr Characterization ◽  
Bond Network
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