Superfast Tetrazole–BCN Cycloaddition Reaction for Bioorthogonal Protein Labeling on Live Cells

2021 ◽  
Author(s):  
Gangam Srikanth Kumar ◽  
Stefano Racioppi ◽  
Eva Zurek ◽  
Qing Lin
Keyword(s):  
Cycloaddition Reaction ◽  
Protein Labeling ◽  
Live Cells

Recognition-driven chemical labeling of endogenous proteins in multi-molecular crowding in live cells

2017 ◽  
Vol 53 (88) ◽  
pp. 11972-11983 ◽  
Author(s):  
Kazuma Amaike ◽  
Tomonori Tamura ◽  
Itaru Hamachi
Keyword(s):  
Protein Labeling ◽  
Live Cells ◽  
Molecular Crowding ◽  
Chemical Labeling ◽  
Endogenous Protein ◽  
Bona Fide ◽  
Endogenous Proteins

Endogenous protein labeling is one of the most invaluable methods for studying the bona fide functions of proteins in live cells.

scholarly journals Collagen labelling with an azide-proline chemical reporter in live cells

2015 ◽  
Vol 51 (25) ◽  
pp. 5250-5252 ◽  
Author(s):  
Beatrice Amgarten ◽  
Rakesh Rajan ◽  
Nuria Martínez-Sáez ◽  
Bruno L. Oliveira ◽  
Inês S. Albuquerque ◽  
...  
Keyword(s):  
Cycloaddition Reaction ◽  
Live Cells ◽  
Chemical Reporter

Biosynthetic incorporation of an azide-proline chemical reporter into collagen allows selective imaging in live foetal ovine osteoblasts using a strain-promoted [3+2] azide–alkyne cycloaddition reaction.

scholarly journals Sterically Shielded, Stabilized Nitrile Imine for Rapid Bioorthogonal Protein Labeling in Live Cells

2018 ◽  
Vol 140 (14) ◽  
pp. 4860-4868 ◽  
Author(s):  
Peng An ◽  
Tracey M. Lewandowski ◽  
Tuğçe G. Erbay ◽  
Peng Liu ◽  
Qing Lin
Keyword(s):  
Protein Labeling ◽  
Live Cells ◽  
Nitrile Imine

Protein Labeling in Live Cells for Immunological Applications

2018 ◽  
Vol 29 (3) ◽  
pp. 680-685 ◽  
Author(s):  
Parisa Moghaddam-Taaheri ◽  
Amy J. Karlsson
Keyword(s):  
Protein Labeling ◽  
Live Cells

scholarly journals Rational Development of Caged-Biotin Protein-Labeling Agents and Some Applications in Live Cells

2011 ◽  
Vol 18 (10) ◽  
pp. 1261-1272 ◽  
Author(s):  
Takuya Terai ◽  
Eri Maki ◽  
Shigeru Sugiyama ◽  
Yoshinori Takahashi ◽  
Hiroyoshi Matsumura ◽  
...  
Keyword(s):  
Protein Labeling ◽  
Live Cells ◽  
Rational Development

A novel reactive turn-on probe capable of selective profiling and no-wash imaging of Bruton's tyrosine kinase in live cells

2019 ◽  
Vol 55 (24) ◽  
pp. 3473-3476 ◽  
Author(s):  
Xin Wang ◽  
Nan Ma ◽  
Rui Wu ◽  
Ke Ding ◽  
Zhengqiu Li
Keyword(s):  
Tyrosine Kinase ◽  
Mammalian Cells ◽  
High Sensitivity ◽  
Protein Labeling ◽  
Live Cells ◽  
Bruton’S Tyrosine Kinase ◽  
Bruton's Tyrosine Kinase ◽  
Turn On ◽  
Sensitivity And Selectivity

A series of reaction-based probes have been developed by conjugation of maleimide–coumarin into ibrutinib. The resulting probes display high sensitivity and selectivity toward BTK, and were proven to be suitable for simultaneous protein labeling and no-wash imaging of BTK inside live mammalian cells.

scholarly journals Selective Mitochondrial Protein Labeling Enabled by Biocompatible Photocatalytic Reactions inside Live Cells

JACS Au ◽  
2021 ◽  
Author(s):  
Haoyan Wang ◽  
Yixin Zhang ◽  
Kaixing Zeng ◽  
Jiali Qiang ◽  
Ye Cao ◽  
...  
Keyword(s):  
Mitochondrial Protein ◽  
Protein Labeling ◽  
Live Cells ◽  
Photocatalytic Reactions

Sydnone-based turn-on fluorogenic probes for no-wash protein labeling and in-cell imaging

2019 ◽  
Vol 55 (31) ◽  
pp. 4582-4585 ◽  
Author(s):  
Lucie Plougastel ◽  
Manas R. Pattanayak ◽  
Margaux Riomet ◽  
Sarah Bregant ◽  
Antoine Sallustrau ◽  
...  
Keyword(s):  
Cell Imaging ◽  
Cycloaddition Reaction ◽  
Protein Labeling ◽  
Turn On ◽  
Fluorogenic Probes

Fluorogenic sydnone-based turn-on probes allow efficient labeling of proteins and cell imaging through a bioorthogonal strained promoted sydnone–alkyne cycloaddition reaction.

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