Qualitative and Quantitative Event-Specific PCR Detection Methods for Oxy-235 Canola Based on the 3′ Integration Flanking Sequence

2008 ◽  
Vol 56 (6) ◽  
pp. 1804-1809 ◽  
Author(s):  
Litao Yang ◽  
Jinchao Guo ◽  
Haibo Zhang ◽  
Jia Liu ◽  
Dabing Zhang
Keyword(s):  
Pcr Detection ◽  
Detection Methods ◽  
Flanking Sequence ◽  
Qualitative And Quantitative ◽  
Specific Pcr

Collaborative trial for the validation of event-specific PCR detection methods of genetically modified papaya Huanong No.1

2016 ◽  
Vol 194 ◽  
pp. 20-25 ◽  
Author(s):  
Jiaojun Wei ◽  
Huangying Le ◽  
Aihu Pan ◽  
Junfeng Xu ◽  
Feiwu Li ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Pcr Detection ◽  
Detection Methods ◽  
Collaborative Trial ◽  
Specific Pcr

Characterization of the Exogenous Insert and Development of Event-specific PCR Detection Methods for Genetically Modified Huanong No. 1 Papaya

2009 ◽  
Vol 57 (16) ◽  
pp. 7205-7212 ◽  
Author(s):  
Jinchao Guo ◽  
Litao Yang ◽  
Xin Liu ◽  
Xiaoyan Guan ◽  
Lingxi Jiang ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Pcr Detection ◽  
Detection Methods ◽  
Specific Pcr

scholarly journals The virome of German bats: comparing virus discovery approaches

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Claudia Kohl ◽  
Annika Brinkmann ◽  
Aleksandar Radonić ◽  
Piotr Wojtek Dabrowski ◽  
Kristin Mühldorfer ◽  
...  
Keyword(s):  
Virus Detection ◽  
Detection Methods ◽  
Single Type ◽  
Metagenomic Sequencing ◽  
Virus Discovery ◽  
Highly Pathogenic ◽  
Pcr Screening ◽  
Specific Pcr ◽  
Pathogenic Viruses ◽  
Viral Sequences

AbstractBats are known to be reservoirs of several highly pathogenic viruses. Hence, the interest in bat virus discovery has been increasing rapidly over the last decade. So far, most studies have focused on a single type of virus detection method, either PCR, virus isolation or virome sequencing. Here we present a comprehensive approach in virus discovery, using all three discovery methods on samples from the same bats. By family-specific PCR screening we found sequences of paramyxoviruses, adenoviruses, herpesviruses and one coronavirus. By cell culture we isolated a novel bat adenovirus and bat orthoreovirus. Virome sequencing revealed viral sequences of ten different virus families and orders: three bat nairoviruses, three phenuiviruses, one orbivirus, one rotavirus, one orthoreovirus, one mononegavirus, five parvoviruses, seven picornaviruses, three retroviruses, one totivirus and two thymoviruses were discovered. Of all viruses identified by family-specific PCR in the original samples, none was found by metagenomic sequencing. Vice versa, none of the viruses found by the metagenomic virome approach was detected by family-specific PCRs targeting the same family. The discrepancy of detected viruses by different detection approaches suggests that a combined approach using different detection methods is necessary for virus discovery studies.

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