A Well-Refined In Vitro Model Derived from Human Embryonic Stem Cell for Screening Phytochemicals with Midbrain Dopaminergic Differentiation-Boosting Potential for Improving Parkinson’s Disease

2014 ◽  
Vol 62 (27) ◽  
pp. 6326-6336 ◽  
Author(s):  
Wen-Ting Hsieh ◽  
Been-Huang Chiang
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
In Vitro Model ◽  
Embryonic Stem ◽  
Vitro Model ◽  
Dopaminergic Differentiation

scholarly journals Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Miranda Robbins ◽  
Venkat Pisupati ◽  
Roberta Azzarelli ◽  
Samer I. Nehme ◽  
Roger A. Barker ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Bacterial Cellulose ◽  
Progenitor Cells ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem

Abstract Background Stem cell-based therapies for neurodegenerative diseases like Parkinson’s disease are a promising approach in regenerative medicine and are now moving towards early stage clinical trials. However, a number of challenges remain including the ability to grow stem cells in vitro on a 3-dimensional scaffold, as well as their loss, by leakage or cell death, post-implantation. These issues could, however, be helped through the use of scaffolds that support the growth and differentiation of stem cells both in vitro and in vivo. The present study focuses on the use of bacterial cellulose as an in vitro scaffold to promote the growth of different stem cell-derived cell types. Bacterial cellulose was used because of its remarkable properties such as its wettability, ability to retain water and low stiffness, all of which is similar to that found in brain tissue. Methods We cultured human embryonic stem cell-derived progenitor cells on bacterial cellulose with growth factors that were covalently functionalised to the surface via silanisation. Epifluorescence microscopy and immunofluorescence were used to detect the differentiation of stem cells into dopaminergic ventral midbrain progenitor cells. We then quantified the proportion of cells that differentiated into progenitor cells and compared the effect of growing cells on biofunctionalised cellulose versus standard cellulose. Results We show that the covalent functionalisation of bacterial cellulose sheets with bioactive peptides improves the growth and differentiation of human pluripotent stem cells into dopaminergic neuronal progenitors. Conclusions This study suggests that the biocompatible material, bacterial cellulose, has potential applications in cell therapy approaches as a means to repair damage to the central nervous system, such as in Parkinson’s disease but also in tissue engineering.

scholarly journals Human Embryonic Stem Cell-Derived Epithelial Cells in a Novel In Vitro Model of Vocal Mucosa

2013 ◽  
Vol 19 (19-20) ◽  
pp. 2233-2241 ◽  
Author(s):  
Ciara Leydon ◽  
Joshua A. Selekman ◽  
Sean Palecek ◽  
Susan L. Thibeault
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
In Vitro Model ◽  
Embryonic Stem ◽  
Vitro Model

scholarly journals Engraftment of human embryonic stem cell derived cardiomyocytes improves conduction in an arrhythmogenic in vitro model

2012 ◽  
Vol 53 (1) ◽  
pp. 15-23 ◽  
Author(s):  
Susan A. Thompson ◽  
Paul W. Burridge ◽  
Elizabeth A. Lipke ◽  
Michael Shamblott ◽  
Elias T. Zambidis ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
In Vitro Model ◽  
Embryonic Stem ◽  
Vitro Model

scholarly journals Aggregate Culture of Human Embryonic Stem Cell-Derived Hepatocytes in Suspension Are an Improved In Vitro Model for Drug Metabolism and Toxicity Testing

2014 ◽  
Vol 140 (1) ◽  
pp. 236-245 ◽  
Author(s):  
Srikumar Sengupta ◽  
Brian Patrick Johnson ◽  
Scott Allen Swanson ◽  
Ron Stewart ◽  
Christopher Alan Bradfield ◽  
...  
Keyword(s):  
Stem Cell ◽  
Drug Metabolism ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
In Vitro Model ◽  
Embryonic Stem ◽  
Toxicity Testing ◽  
Aggregate Culture ◽  
Vitro Model

scholarly journals A novel in vitro model system for smooth muscle differentiation from human embryonic stem cell-derived mesenchymal cells

2013 ◽  
Vol 304 (4) ◽  
pp. C289-C298 ◽  
Author(s):  
Xia Guo ◽  
Steven L. Stice ◽  
Nolan L. Boyd ◽  
Shi-You Chen
Keyword(s):  
Smooth Muscle ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
In Vitro Model ◽  
Embryonic Stem ◽  
Mesenchymal Cells ◽  
Dependent Manner ◽  
Vitro Model

The objective of this study was to develop a novel in vitro model for smooth muscle cell (SMC) differentiation from human embryonic stem cell-derived mesenchymal cells (hES-MCs). We found that hES-MCs were differentiated to SMCs by transforming growth factor-β (TGF-β) in a dose- and time-dependent manner as demonstrated by the expression of SMC-specific genes smooth muscle α-actin, calponin, and smooth muscle myosin heavy chain. Under normal growth conditions, however, the differentiation capacity of hES-MCs was very limited. hES-MC-derived SMCs had an elongated and spindle-shaped morphology and contracted in response to the induction of carbachol and KCl. KCl-induced calcium transient was also evident in these cells. Compared with the parental cells, TGF-β-treated hES-MCs sustained the endothelial tube formation for a longer time due to the sustained SMC phenotype. Mechanistically, TGF-β-induced differentiation was both Smad- and serum response factor/myocardin dependent. TGF-β regulated myocardin expression via multiple signaling pathways including Smad2/3, p38 MAPK, and PI3K. Importantly, we found that a low level of myocardin was present in mesoderm prior to SMC lineage determination, and a high level of myocardin was not induced until the differentiation process was initiated. Taken together, our study characterized a novel SMC differentiation model that can be used for studying human SMC differentiation from mesoderm during vascular development.

scholarly journals Human embryonic stem cell-derived cardiomyocytes as an in vitro model to study cardiac insulin resistance

2018 ◽  
Vol 1864 (5) ◽  
pp. 1960-1967 ◽  
Author(s):  
Ilvy M.E. Geraets ◽  
Dipanjan Chanda ◽  
Florence H.J. van Tienen ◽  
Arthur van den Wijngaard ◽  
Rick Kamps ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
In Vitro Model ◽  
Embryonic Stem ◽  
Vitro Model

scholarly journals Potential for cell therapy in Parkinson's disease using genetically programmed human embryonic stem cell-derived neural progenitor cells

2014 ◽  
Vol 522 (12) ◽  
pp. 2845-2856 ◽  
Author(s):  
Rajesh Ambasudhan ◽  
Nima Dolatabadi ◽  
Anthony Nutter ◽  
Eliezer Masliah ◽  
Scott R. Mckercher ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Stem Cell ◽  
Cell Therapy ◽  
Embryonic Stem Cell ◽  
Progenitor Cells ◽  
Human Embryonic Stem Cell ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Neural Progenitor
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