Glassy Character of DNA Hydration Water

The aqueous environment and ionic surrounding are the most important factors determining the conformation of DNA and its functioning in the cell. The specificity of the interaction between DNA and cations is especially pronounced with a decrease in water activity. In this work, we studied the B-A transition in high molecular weight DNA with a decrease of humidity in the film with different contents of Na+ ions using FTIR spectroscopy. The IR spectrum of DNA is not only very sensitive to the state of its secondary structure, but also allows us to estimate the amount of water bound to DNA. Upon dehydration of the DNA film, changes characteristic of the B-A transition were observed in the IR absorption spectrum. Using thermogravimetric analysis, it was shown that the degree of DNA hydration reaches the saturation level at a relative humidity of 60% and decreases slightly upon further drying. It has been established that with increasing Na+ concentration, the amount of water strongly bound to DNA decreases. Along with it, sodium ions destroy the hydration shell of DNA and are able to interact directly with phosphate groups.

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Faculty Opinions recommendation of How protein surfaces induce anomalous dynamics of hydration water.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1089312.542526 ◽

2007 ◽

Author(s):

Bernard Montgomery Pettitt

Keyword(s):

Hydration Water ◽

Protein Surfaces ◽

Anomalous Dynamics

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Site-Specific Coupling of Hydration Water and Protein Flexibility Studied in Solution with Ultrafast 2D-IR Spectroscopy

Journal of the American Chemical Society ◽

10.1021/ja307401r ◽

2012 ◽

Vol 134 (45) ◽

pp. 18705-18712 ◽

Cited By ~ 110

Author(s):

John T. King ◽

Kevin J. Kubarych

Keyword(s):

Ir Spectroscopy ◽

Protein Flexibility ◽

Hydration Water ◽

Site Specific ◽

2D Ir

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Fluorescence decay time measurements of Eu3+-ATP-enzyme complexes. Replacement of the metal hydration water by active site ligands.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)44144-5 ◽

1983 ◽

Vol 258 (20) ◽

pp. 12132-12134

Author(s):

M Gutman ◽

M A Levy

Keyword(s):

Decay Time ◽

Active Site ◽

Fluorescence Decay ◽

Hydration Water ◽

Fluorescence Decay Time ◽

Enzyme Complexes

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Successive Crystallization of Organically Templated Uranyl Sulfates: Synthesis and Crystal Structures of [pyH](H3O)[(UO2)3(SO4)4(H2O)2], [pyH]2[(UO2)6(SO4)7(H2O)], and [pyH]2[(UO2)2(SO4)3]

ChemEngineering ◽

10.3390/chemengineering5010005 ◽

2021 ◽

Vol 5 (1) ◽

pp. 5

Author(s):

Evgeny V. Nazarchuk ◽

Dmitri O. Charkin ◽

Oleg I. Siidra

Keyword(s):

Hydrogen Bonds ◽

Crystal Structures ◽

Hydrothermal Treatment ◽

Mother Liquor ◽

Hydration Water ◽

Isothermal Evaporation ◽

Pyridinium Cations

Three new uranyl sulfates, [pyH](H3O)[(UO2)3(SO4)4(H2O)2] (1), [pyH]2[(UO2)6(SO4)7(H2O)] (2), and [pyH]2[(UO2)2(SO4)3] (3), were produced upon hydrothermal treatment and successive isothermal evaporation. 1 is monoclinic, P21/c, a = 14.3640(13), b = 10.0910(9), c = 18.8690(17) Å, β = 107.795(2), V = 2604.2(4) Å3, R1 = 0.038; 2 is orthorhombic, C2221, a = 10.1992(8), b = 18.5215(14), c = 22.7187(17) Å, V = 4291.7(6) Å3, R1 = 0.030; 3 is orthorhombic, Pccn, a = 9.7998(8), b = 10.0768(8), c = 20.947(2) Å, V = 2068.5(3) Å3, R1 = 0.055. In the structures of 1 and 2, the uranium polyhedra and SO4 tetrahedra share vertices to form ∞3[(UO2)3(SO4)4(H2O)2]2− and ∞3[(UO2)6(SO4)7(H2O)]2− frameworks featuring channels (12.2 × 6.7 Å in 1 and 12.9 × 6.5 Å in 2), which are occupied by pyridinium cations. The structure of 3 is comprised of ∞2[(UO2)2(SO4)3]2− layers linked by hydrogen bonds donated by pyridinium cations. The compounds 1–3 are formed during recrystallization processes, in which the evaporation of mother liquor leads to a stepwise loss of hydration water.

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