Combining Total Internal Reflection Sum Frequency Spectroscopy Spectral Imaging and Confocal Fluorescence Microscopy

Langmuir ◽  
2015 ◽  
Vol 31 (3) ◽  
pp. 987-994 ◽  
Author(s):  
Edward S. Allgeyer ◽  
Sarah M. Sterling ◽  
Mudalige S. Gunewardene ◽  
Samuel T. Hess ◽  
David J. Neivandt ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Spectral Imaging ◽  
Internal Reflection ◽  
Confocal Fluorescence Microscopy ◽  
Sum Frequency ◽  
Sum Frequency Spectroscopy ◽  
Confocal Fluorescence

Total Internal Reflection Sum-Frequency Spectroscopy:  A Strategy for Studying Molecular Adsorption on Metal Surfaces

Langmuir ◽  
2000 ◽  
Vol 16 (5) ◽  
pp. 2343-2350 ◽  
Author(s):  
Christopher T. Williams ◽  
Yong Yang ◽  
Colin D. Bain
Keyword(s):  
Total Internal Reflection ◽  
Metal Surfaces ◽  
Internal Reflection ◽  
Molecular Adsorption ◽  
Sum Frequency ◽  
Sum Frequency Spectroscopy

Aliphatic Nitrile Adsorption on Al2O3and ZrO2As Studied by Total Internal Reflection Sum-Frequency Spectroscopy

Langmuir ◽  
2003 ◽  
Vol 19 (22) ◽  
pp. 9210-9215 ◽  
Author(s):  
Michael R. Strunk ◽  
Christopher T. Williams
Keyword(s):  
Total Internal Reflection ◽  
Internal Reflection ◽  
Sum Frequency ◽  
Sum Frequency Spectroscopy ◽  
Aliphatic Nitrile

Hyper-spectral imaging in scanning-confocal-fluorescence microscopy using a novel broadband diffractive optic

2014 ◽  
Vol 324 ◽  
pp. 73-80 ◽  
Author(s):  
Peng Wang ◽  
Carl G. Ebeling ◽  
Jordan Gerton ◽  
Rajesh Menon
Keyword(s):  
Fluorescence Microscopy ◽  
Spectral Imaging ◽  
Confocal Fluorescence Microscopy ◽  
Diffractive Optic ◽  
Hyper Spectral ◽  
Confocal Fluorescence ◽  
Hyper Spectral Imaging

Functional Fluorescence Microscopy Imaging: Quantitative Scanning-Free Confocal Fluorescence Microscopy for the Characterization of Fast Dynamic Processes in Live Cells

2019 ◽  
Vol 91 (17) ◽  
pp. 11129-11137 ◽  
Author(s):  
Aleksandar J. Krmpot ◽  
Stanko N. Nikolić ◽  
Sho Oasa ◽  
Dimitrios K. Papadopoulos ◽  
Marco Vitali ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Confocal Fluorescence Microscopy ◽  
Live Cells ◽  
Dynamic Processes ◽  
Microscopy Imaging ◽  
Confocal Fluorescence ◽  
Fast Dynamic

Choline and phosphatidylcholine fluorescent derivatives localization in carcinoma cells studied by laser scanning confocal fluorescence microscopy

2005 ◽  
Vol 41 (10) ◽  
pp. 1453-1459 ◽  
Author(s):  
Anna M. Villa ◽  
Elvira Caporizzo ◽  
Antonio Papagni ◽  
Luciano Miozzo ◽  
Paola Del Buttero ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Laser Scanning ◽  
Carcinoma Cells ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence ◽  
Fluorescent Derivatives

scholarly journals Analysis of Polyhydroxyalkanoates Granules in Haloferax mediterranei by Double-Fluorescence Staining with Nile Red and SYBR Green by Confocal Fluorescence Microscopy

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1582
Author(s):  
Verónica Cánovas ◽  
Salvador Garcia-Chumillas ◽  
Fuensanta Monzó ◽  
Lorena Simó-Cabrera ◽  
Carmen Fernández-Ayuso ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Culture Media ◽  
Nile Red ◽  
Sybr Green ◽  
Confocal Fluorescence Microscopy ◽  
Fluorescence Staining ◽  
Haloferax Mediterranei ◽  
Optimized Protocol ◽  
Confocal Fluorescence ◽  
Pha Production

Haloferaxmediterranei is a haloarchaeon of high interest in biotechnology because it produces and mobilizes intracellular polyhydroxyalkanoate (PHA) granules during growth under stress conditions (limitation of phosphorous in the culture media), among other interesting metabolites (enzymes, carotenoids, etc.). The capability of PHA production by microbes can be monitored with the use of staining-based methods. However, the staining of haloarchaea cells is a challenging task; firstly, due to the high ionic strength of the medium, which is inappropriate for most of dyes, and secondly, due to the low permeability of the haloarchaea S-layer to macromolecules. In this work, Haloferax mediterranei is used as a halophilic archaeon model to describe an optimized protocol for the visualization and analysis of intracellular PHA granules in living cells. The method is based on double-fluorescence staining using Nile red and SYBR Green by confocal fluorescence microscopy. Thanks to this method, the capability of PHA production by new haloarchaea isolates could be easily monitored.

Analysis of Sarcomere Lenght Oscillation in Cardiac Muscle Fibers by Confocal Fluorescence Microscopy

1999 ◽  
Vol 39 (supplement) ◽  
pp. S195
Author(s):  
D. Sasaki ◽  
H. Fujita ◽  
S. Ishiwata
Keyword(s):  
Fluorescence Microscopy ◽  
Cardiac Muscle ◽  
Muscle Fibers ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence ◽  
Cardiac Muscle Fibers
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