Fluorescence Lifetime Imaging Microscopy for the Detection of Intracellular pH with Quantum Dot Nanosensors

ACS Nano ◽  
2013 ◽  
Vol 7 (7) ◽  
pp. 6387-6395 ◽  
Author(s):  
Angel Orte ◽  
Jose M. Alvarez-Pez ◽  
Maria J. Ruedas-Rama
ACS Sensors ◽  
2020 ◽  
Vol 5 (7) ◽  
pp. 2106-2117 ◽  
Author(s):  
Pedro J. Pacheco-Liñán ◽  
Iván Bravo ◽  
María L. Nueda ◽  
José Albaladejo ◽  
Andrés Garzón-Ruiz

2014 ◽  
Vol 2 (39) ◽  
pp. 6792-6801 ◽  
Author(s):  
D. Aigner ◽  
R. I. Dmitriev ◽  
S. M. Borisov ◽  
D. B. Papkovsky ◽  
I. Klimant

Several new perylene bisimide (PBI) probes comprising oligo-guanidine conjugates and cationic hydrogel nanoparticle structures were designed for sensing intracellular pH in live cell fluorescence lifetime imaging microscopy (FLIM).


Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 604 ◽  
Author(s):  
Maojia Huang ◽  
Xinyue Liang ◽  
Zixiao Zhang ◽  
Jing Wang ◽  
Yiyan Fei ◽  
...  

The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study. In this research, pH-sensitive carbon dots (CDs) were employed for intracellular pH sensing with fluorescence lifetime imaging microscopy (FLIM) for the first time. FLIM is a highly sensitive method that is used to detect a microenvironment and it can overcome the limitations of biosensing methods based on fluorescence intensity. The different groups on the CDs surfaces changing with pH environments led to different fluorescence lifetime values. The CDs aqueous solution had a gradual change from 1.6 ns to 3.7 ns in the fluorescence lifetime with a pH range of 2.6–8.6. Similar fluorescence lifetime changes were found in pH buffer-treated living cells. The detection of lysosomes, cytoplasm, and nuclei in living cells was achieved by measuring the fluorescence lifetime of CDs. In particular, a phasor FLIM analysis was used to improve the pH imaging. Moreover, the effects of the coenzymes, amino acids, and proteins on the fluorescence lifetime of CDs were examined in order to mimic the complex microenvironment inside the cells.


2015 ◽  
Vol 51 (95) ◽  
pp. 16964-16967 ◽  
Author(s):  
Consuelo Ripoll ◽  
Miguel Martin ◽  
Mar Roldan ◽  
Eva M. Talavera ◽  
Angel Orte ◽  
...  

Fluorescence Lifetime Imaging Microscopy (FLIM) has been employed for the detection of intracellular Zn2+ levels, implicated in various signalling pathways, using a family of quantum dot (QD) nanosensors.


2016 ◽  
Vol 52 (24) ◽  
pp. 4458-4461 ◽  
Author(s):  
Hideki Itoh ◽  
Satoshi Arai ◽  
Thankiah Sudhaharan ◽  
Sung-Chan Lee ◽  
Young-Tae Chang ◽  
...  

FLIM of ER thermo yellow and non-targeted mCherry reveals the Ca2+-dependent heat production localized to SR in C2C12 myotube.


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