Proteomic Analysis of Ubiquitinated Proteins from Human MCF-7 Breast Cancer Cells by Immunoaffinity Purification and Mass Spectrometry

2005 ◽  
Vol 4 (6) ◽  
pp. 2192-2200 ◽  
Author(s):  
Julian Vasilescu ◽  
Jeffrey C. Smith ◽  
Martin Ethier ◽  
Daniel Figeys
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Cancer Cells ◽  
Proteomic Analysis ◽  
Breast Cancer Cells ◽  
Ubiquitinated Proteins ◽  
Immunoaffinity Purification ◽  
Mcf 7

scholarly journals A proteomic analysis of chemoresistance development via sequential treatment with doxorubicin reveals novel players in MCF‑7 breast cancer cells

2018 ◽  
Author(s):  
Ann‑Katrin Sommer ◽  
Adam Hermawan ◽  
Bojan Ljepoja ◽  
Thomas Fr�hlich ◽  
Georg Arnold ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Proteomic Analysis ◽  
Breast Cancer Cells ◽  
Sequential Treatment ◽  
Mcf 7

scholarly journals Quantitative proteomic analysis of the association between decreasing O‑GlcNAcylation and metastasis in MCF‑7 breast cancer cells

2020 ◽  
Author(s):  
Pukkavadee Netsirisawan ◽  
Daranee Chokchaichamnankit ◽  
Kittirat Saharat ◽  
Chantragan Srisomsap ◽  
Jisnuson Svasti ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Proteomic Analysis ◽  
Breast Cancer Cells ◽  
Quantitative Proteomic Analysis ◽  
Mcf 7

Quantitative proteomic analysis of HER2 normal and overexpressing MCF-7 breast cancer cells revealed proteomic changes accompanied with HER2 gene amplification

2013 ◽  
Vol 91 ◽  
pp. 200-209 ◽  
Author(s):  
Yanan Tang ◽  
John Mackey ◽  
Raymond Lai ◽  
Sunita Ghosh ◽  
Cheryl Santos ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Gene Amplification ◽  
Proteomic Analysis ◽  
Breast Cancer Cells ◽  
Her2 Gene ◽  
Quantitative Proteomic Analysis ◽  
Her2 Gene Amplification ◽  
Mcf 7

scholarly journals Comparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mohammad H. Semreen ◽  
Hasan Y. Alniss ◽  
Stefan R. Grgic ◽  
Raafat A. El-Awady ◽  
Ahmed H. Almehdi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Cancer Cells ◽  
Energy Production ◽  
Breast Cancer Cells ◽  
Tumour Progression ◽  
Ammonium Hydroxide ◽  
Extraction Solvent ◽  
Extraction Solvents ◽  
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Abstract Metabolic profiling of cancer cells can play a vital role in revealing the molecular bases of cancer development and progression. In this study, gas chromatography coupled with mass spectrometry (GC-MS) was employed for the determination of signatures found in ER+/PR+ breast cancer cells derived from MCF-7 using different extraction solvents including: A, formic acid in water; B, ammonium hydroxide in water; C, ethyl acetate; D, methanol: water (1:1, v/v); and E, acetonitrile: water (1:1, v/v). The greatest extraction rate and diversity of metabolites occurs with extraction solvents A and E. Extraction solvent D showed moderate extraction efficiency, whereas extraction solvent B and C showed inferior metabolite diversity. Metabolite set enrichment analysis (MSEA) results showed energy production pathways to be key in MCF-7 cell lines. This study showed that mass spectrometry could identify key metabolites associated with cancers. The highest enriched pathways were related to energy production as well as Warburg effect pathways, which may shed light on how energy metabolism has been hijacked to encourage tumour progression and eventually metastasis in breast cancer.

Integrated transcriptomic and proteomic analysis of microplasts derived from macrophage‐conditioned medium‐treated MCF‐7 breast cancer cells

FEBS Letters ◽  
2021 ◽  
Author(s):  
Pooja Kamal Melwani ◽  
Murali Mohan Sagar Balla ◽  
S. Nishad ◽  
Mahesh Padwal ◽  
Rajesh Kumar Chaurasia ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Conditioned Medium ◽  
Proteomic Analysis ◽  
Breast Cancer Cells ◽  
Mcf 7

Mechanism research on the lupeol treatment on MCF-7 breast cancer cells based on cell metabonomics

2014 ◽  
Vol 32 (3) ◽  
pp. 278
Author(s):  
Dongdong SHI ◽  
Yuanyuan KUANG ◽  
Guiming WANG ◽  
Zhangxiao PENG ◽  
Yan WANG ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Mechanism Research ◽  
Mcf 7
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